A Simple and Rapid Method for DNA Isolation from Xylophagous Insects

Published methods to isolate DNA from insects are not always effective in xylophagous insects because they have high concentrations of phenolics and other secondary plant compounds in their digestive tracts. A simple, reliable and labor-effective cetyltrimethylammonium bromide-polyvinylpyrrolidone (...

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Detalles Bibliográficos
Autores principales: Calderón-Cortés, Nancy, Quesada, Mauricio, Cano-Camacho, Horacio, Zavala-Páramo, Guadalupe
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2010
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3100841/
https://www.ncbi.nlm.nih.gov/pubmed/21614191
http://dx.doi.org/10.3390/ijms11125056
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author Calderón-Cortés, Nancy
Quesada, Mauricio
Cano-Camacho, Horacio
Zavala-Páramo, Guadalupe
author_facet Calderón-Cortés, Nancy
Quesada, Mauricio
Cano-Camacho, Horacio
Zavala-Páramo, Guadalupe
author_sort Calderón-Cortés, Nancy
collection PubMed
description Published methods to isolate DNA from insects are not always effective in xylophagous insects because they have high concentrations of phenolics and other secondary plant compounds in their digestive tracts. A simple, reliable and labor-effective cetyltrimethylammonium bromide-polyvinylpyrrolidone (CTAB-PVP) method for isolation of high quality DNA from xylophagous insects is described. This method was successfully applied to PCR and restriction analysis, indicating removal of common inhibitors. DNA isolated by the CTAB-PVP method could be used in most molecular analyses.
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spelling pubmed-31008412011-05-25 A Simple and Rapid Method for DNA Isolation from Xylophagous Insects Calderón-Cortés, Nancy Quesada, Mauricio Cano-Camacho, Horacio Zavala-Páramo, Guadalupe Int J Mol Sci Technical Note Published methods to isolate DNA from insects are not always effective in xylophagous insects because they have high concentrations of phenolics and other secondary plant compounds in their digestive tracts. A simple, reliable and labor-effective cetyltrimethylammonium bromide-polyvinylpyrrolidone (CTAB-PVP) method for isolation of high quality DNA from xylophagous insects is described. This method was successfully applied to PCR and restriction analysis, indicating removal of common inhibitors. DNA isolated by the CTAB-PVP method could be used in most molecular analyses. Molecular Diversity Preservation International (MDPI) 2010-12-07 /pmc/articles/PMC3100841/ /pubmed/21614191 http://dx.doi.org/10.3390/ijms11125056 Text en © 2010 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Technical Note
Calderón-Cortés, Nancy
Quesada, Mauricio
Cano-Camacho, Horacio
Zavala-Páramo, Guadalupe
A Simple and Rapid Method for DNA Isolation from Xylophagous Insects
title A Simple and Rapid Method for DNA Isolation from Xylophagous Insects
title_full A Simple and Rapid Method for DNA Isolation from Xylophagous Insects
title_fullStr A Simple and Rapid Method for DNA Isolation from Xylophagous Insects
title_full_unstemmed A Simple and Rapid Method for DNA Isolation from Xylophagous Insects
title_short A Simple and Rapid Method for DNA Isolation from Xylophagous Insects
title_sort simple and rapid method for dna isolation from xylophagous insects
topic Technical Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3100841/
https://www.ncbi.nlm.nih.gov/pubmed/21614191
http://dx.doi.org/10.3390/ijms11125056
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