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An Adsorptive Transfer Technique Coupled with Brdicka Reaction to Reveal the Importance of Metallothionein in Chemotherapy with Platinum Based Cytostatics
The drugs based on platinum metals represent one of the oldest, but also one of the most effective groups of chemotherapeutic agents. Thanks to many clinical studies it is known that resistance of tumor cells to drugs is a frequent cause of chemotherapy failure. With regard to platinum based drugs,...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Molecular Diversity Preservation International (MDPI)
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3100849/ https://www.ncbi.nlm.nih.gov/pubmed/21614176 http://dx.doi.org/10.3390/ijms11124826 |
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author | Krizkova, Sona Fabrik, Ivo Huska, Dalibor Adam, Vojtech Babula, Petr Hrabeta, Jan Eckschlager, Tomas Pochop, Pavel Darsova, Denisa Kukacka, Jiri Prusa, Richard Trnkova, Libuse Kizek, Rene |
author_facet | Krizkova, Sona Fabrik, Ivo Huska, Dalibor Adam, Vojtech Babula, Petr Hrabeta, Jan Eckschlager, Tomas Pochop, Pavel Darsova, Denisa Kukacka, Jiri Prusa, Richard Trnkova, Libuse Kizek, Rene |
author_sort | Krizkova, Sona |
collection | PubMed |
description | The drugs based on platinum metals represent one of the oldest, but also one of the most effective groups of chemotherapeutic agents. Thanks to many clinical studies it is known that resistance of tumor cells to drugs is a frequent cause of chemotherapy failure. With regard to platinum based drugs, multidrug resistance can also be connected with increased expression of low-molecular weight protein metallothionein (MT). This study aimed at investigating the interactions of MT with cisplatin or carboplatin, using the adsorptive transfer technique coupled with differential pulse voltammetry Brdicka reaction (AdTS DPV Brdicka reaction), and a comparison of in vitro results with results obtained in vivo. The results obtained from the in vitro study show a strong affinity between platinum based drugs and MT. Further, we analyzed extracts of neuroblastoma cell lines treated with cisplatin or carboplatin. It is clear that neuroblastoma UKF-NB-4 cisplatin-resistant and cisplatin-sensitive cell lines unlikely respond to the presence of the platinum-based cytostatics cisplatin and carboplatin. Finally, we determined the level of MT in samples from rabbits treated with carboplatin and patients with retinoblastoma treated with the same drug. |
format | Text |
id | pubmed-3100849 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-31008492011-05-25 An Adsorptive Transfer Technique Coupled with Brdicka Reaction to Reveal the Importance of Metallothionein in Chemotherapy with Platinum Based Cytostatics Krizkova, Sona Fabrik, Ivo Huska, Dalibor Adam, Vojtech Babula, Petr Hrabeta, Jan Eckschlager, Tomas Pochop, Pavel Darsova, Denisa Kukacka, Jiri Prusa, Richard Trnkova, Libuse Kizek, Rene Int J Mol Sci Article The drugs based on platinum metals represent one of the oldest, but also one of the most effective groups of chemotherapeutic agents. Thanks to many clinical studies it is known that resistance of tumor cells to drugs is a frequent cause of chemotherapy failure. With regard to platinum based drugs, multidrug resistance can also be connected with increased expression of low-molecular weight protein metallothionein (MT). This study aimed at investigating the interactions of MT with cisplatin or carboplatin, using the adsorptive transfer technique coupled with differential pulse voltammetry Brdicka reaction (AdTS DPV Brdicka reaction), and a comparison of in vitro results with results obtained in vivo. The results obtained from the in vitro study show a strong affinity between platinum based drugs and MT. Further, we analyzed extracts of neuroblastoma cell lines treated with cisplatin or carboplatin. It is clear that neuroblastoma UKF-NB-4 cisplatin-resistant and cisplatin-sensitive cell lines unlikely respond to the presence of the platinum-based cytostatics cisplatin and carboplatin. Finally, we determined the level of MT in samples from rabbits treated with carboplatin and patients with retinoblastoma treated with the same drug. Molecular Diversity Preservation International (MDPI) 2010-11-26 /pmc/articles/PMC3100849/ /pubmed/21614176 http://dx.doi.org/10.3390/ijms11124826 Text en © 2010 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Krizkova, Sona Fabrik, Ivo Huska, Dalibor Adam, Vojtech Babula, Petr Hrabeta, Jan Eckschlager, Tomas Pochop, Pavel Darsova, Denisa Kukacka, Jiri Prusa, Richard Trnkova, Libuse Kizek, Rene An Adsorptive Transfer Technique Coupled with Brdicka Reaction to Reveal the Importance of Metallothionein in Chemotherapy with Platinum Based Cytostatics |
title | An Adsorptive Transfer Technique Coupled with Brdicka Reaction to Reveal the Importance of Metallothionein in Chemotherapy with Platinum Based Cytostatics |
title_full | An Adsorptive Transfer Technique Coupled with Brdicka Reaction to Reveal the Importance of Metallothionein in Chemotherapy with Platinum Based Cytostatics |
title_fullStr | An Adsorptive Transfer Technique Coupled with Brdicka Reaction to Reveal the Importance of Metallothionein in Chemotherapy with Platinum Based Cytostatics |
title_full_unstemmed | An Adsorptive Transfer Technique Coupled with Brdicka Reaction to Reveal the Importance of Metallothionein in Chemotherapy with Platinum Based Cytostatics |
title_short | An Adsorptive Transfer Technique Coupled with Brdicka Reaction to Reveal the Importance of Metallothionein in Chemotherapy with Platinum Based Cytostatics |
title_sort | adsorptive transfer technique coupled with brdicka reaction to reveal the importance of metallothionein in chemotherapy with platinum based cytostatics |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3100849/ https://www.ncbi.nlm.nih.gov/pubmed/21614176 http://dx.doi.org/10.3390/ijms11124826 |
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