Cargando…

An Adsorptive Transfer Technique Coupled with Brdicka Reaction to Reveal the Importance of Metallothionein in Chemotherapy with Platinum Based Cytostatics

The drugs based on platinum metals represent one of the oldest, but also one of the most effective groups of chemotherapeutic agents. Thanks to many clinical studies it is known that resistance of tumor cells to drugs is a frequent cause of chemotherapy failure. With regard to platinum based drugs,...

Descripción completa

Detalles Bibliográficos
Autores principales: Krizkova, Sona, Fabrik, Ivo, Huska, Dalibor, Adam, Vojtech, Babula, Petr, Hrabeta, Jan, Eckschlager, Tomas, Pochop, Pavel, Darsova, Denisa, Kukacka, Jiri, Prusa, Richard, Trnkova, Libuse, Kizek, Rene
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3100849/
https://www.ncbi.nlm.nih.gov/pubmed/21614176
http://dx.doi.org/10.3390/ijms11124826
_version_ 1782204221893902336
author Krizkova, Sona
Fabrik, Ivo
Huska, Dalibor
Adam, Vojtech
Babula, Petr
Hrabeta, Jan
Eckschlager, Tomas
Pochop, Pavel
Darsova, Denisa
Kukacka, Jiri
Prusa, Richard
Trnkova, Libuse
Kizek, Rene
author_facet Krizkova, Sona
Fabrik, Ivo
Huska, Dalibor
Adam, Vojtech
Babula, Petr
Hrabeta, Jan
Eckschlager, Tomas
Pochop, Pavel
Darsova, Denisa
Kukacka, Jiri
Prusa, Richard
Trnkova, Libuse
Kizek, Rene
author_sort Krizkova, Sona
collection PubMed
description The drugs based on platinum metals represent one of the oldest, but also one of the most effective groups of chemotherapeutic agents. Thanks to many clinical studies it is known that resistance of tumor cells to drugs is a frequent cause of chemotherapy failure. With regard to platinum based drugs, multidrug resistance can also be connected with increased expression of low-molecular weight protein metallothionein (MT). This study aimed at investigating the interactions of MT with cisplatin or carboplatin, using the adsorptive transfer technique coupled with differential pulse voltammetry Brdicka reaction (AdTS DPV Brdicka reaction), and a comparison of in vitro results with results obtained in vivo. The results obtained from the in vitro study show a strong affinity between platinum based drugs and MT. Further, we analyzed extracts of neuroblastoma cell lines treated with cisplatin or carboplatin. It is clear that neuroblastoma UKF-NB-4 cisplatin-resistant and cisplatin-sensitive cell lines unlikely respond to the presence of the platinum-based cytostatics cisplatin and carboplatin. Finally, we determined the level of MT in samples from rabbits treated with carboplatin and patients with retinoblastoma treated with the same drug.
format Text
id pubmed-3100849
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher Molecular Diversity Preservation International (MDPI)
record_format MEDLINE/PubMed
spelling pubmed-31008492011-05-25 An Adsorptive Transfer Technique Coupled with Brdicka Reaction to Reveal the Importance of Metallothionein in Chemotherapy with Platinum Based Cytostatics Krizkova, Sona Fabrik, Ivo Huska, Dalibor Adam, Vojtech Babula, Petr Hrabeta, Jan Eckschlager, Tomas Pochop, Pavel Darsova, Denisa Kukacka, Jiri Prusa, Richard Trnkova, Libuse Kizek, Rene Int J Mol Sci Article The drugs based on platinum metals represent one of the oldest, but also one of the most effective groups of chemotherapeutic agents. Thanks to many clinical studies it is known that resistance of tumor cells to drugs is a frequent cause of chemotherapy failure. With regard to platinum based drugs, multidrug resistance can also be connected with increased expression of low-molecular weight protein metallothionein (MT). This study aimed at investigating the interactions of MT with cisplatin or carboplatin, using the adsorptive transfer technique coupled with differential pulse voltammetry Brdicka reaction (AdTS DPV Brdicka reaction), and a comparison of in vitro results with results obtained in vivo. The results obtained from the in vitro study show a strong affinity between platinum based drugs and MT. Further, we analyzed extracts of neuroblastoma cell lines treated with cisplatin or carboplatin. It is clear that neuroblastoma UKF-NB-4 cisplatin-resistant and cisplatin-sensitive cell lines unlikely respond to the presence of the platinum-based cytostatics cisplatin and carboplatin. Finally, we determined the level of MT in samples from rabbits treated with carboplatin and patients with retinoblastoma treated with the same drug. Molecular Diversity Preservation International (MDPI) 2010-11-26 /pmc/articles/PMC3100849/ /pubmed/21614176 http://dx.doi.org/10.3390/ijms11124826 Text en © 2010 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Krizkova, Sona
Fabrik, Ivo
Huska, Dalibor
Adam, Vojtech
Babula, Petr
Hrabeta, Jan
Eckschlager, Tomas
Pochop, Pavel
Darsova, Denisa
Kukacka, Jiri
Prusa, Richard
Trnkova, Libuse
Kizek, Rene
An Adsorptive Transfer Technique Coupled with Brdicka Reaction to Reveal the Importance of Metallothionein in Chemotherapy with Platinum Based Cytostatics
title An Adsorptive Transfer Technique Coupled with Brdicka Reaction to Reveal the Importance of Metallothionein in Chemotherapy with Platinum Based Cytostatics
title_full An Adsorptive Transfer Technique Coupled with Brdicka Reaction to Reveal the Importance of Metallothionein in Chemotherapy with Platinum Based Cytostatics
title_fullStr An Adsorptive Transfer Technique Coupled with Brdicka Reaction to Reveal the Importance of Metallothionein in Chemotherapy with Platinum Based Cytostatics
title_full_unstemmed An Adsorptive Transfer Technique Coupled with Brdicka Reaction to Reveal the Importance of Metallothionein in Chemotherapy with Platinum Based Cytostatics
title_short An Adsorptive Transfer Technique Coupled with Brdicka Reaction to Reveal the Importance of Metallothionein in Chemotherapy with Platinum Based Cytostatics
title_sort adsorptive transfer technique coupled with brdicka reaction to reveal the importance of metallothionein in chemotherapy with platinum based cytostatics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3100849/
https://www.ncbi.nlm.nih.gov/pubmed/21614176
http://dx.doi.org/10.3390/ijms11124826
work_keys_str_mv AT krizkovasona anadsorptivetransfertechniquecoupledwithbrdickareactiontorevealtheimportanceofmetallothioneininchemotherapywithplatinumbasedcytostatics
AT fabrikivo anadsorptivetransfertechniquecoupledwithbrdickareactiontorevealtheimportanceofmetallothioneininchemotherapywithplatinumbasedcytostatics
AT huskadalibor anadsorptivetransfertechniquecoupledwithbrdickareactiontorevealtheimportanceofmetallothioneininchemotherapywithplatinumbasedcytostatics
AT adamvojtech anadsorptivetransfertechniquecoupledwithbrdickareactiontorevealtheimportanceofmetallothioneininchemotherapywithplatinumbasedcytostatics
AT babulapetr anadsorptivetransfertechniquecoupledwithbrdickareactiontorevealtheimportanceofmetallothioneininchemotherapywithplatinumbasedcytostatics
AT hrabetajan anadsorptivetransfertechniquecoupledwithbrdickareactiontorevealtheimportanceofmetallothioneininchemotherapywithplatinumbasedcytostatics
AT eckschlagertomas anadsorptivetransfertechniquecoupledwithbrdickareactiontorevealtheimportanceofmetallothioneininchemotherapywithplatinumbasedcytostatics
AT pochoppavel anadsorptivetransfertechniquecoupledwithbrdickareactiontorevealtheimportanceofmetallothioneininchemotherapywithplatinumbasedcytostatics
AT darsovadenisa anadsorptivetransfertechniquecoupledwithbrdickareactiontorevealtheimportanceofmetallothioneininchemotherapywithplatinumbasedcytostatics
AT kukackajiri anadsorptivetransfertechniquecoupledwithbrdickareactiontorevealtheimportanceofmetallothioneininchemotherapywithplatinumbasedcytostatics
AT prusarichard anadsorptivetransfertechniquecoupledwithbrdickareactiontorevealtheimportanceofmetallothioneininchemotherapywithplatinumbasedcytostatics
AT trnkovalibuse anadsorptivetransfertechniquecoupledwithbrdickareactiontorevealtheimportanceofmetallothioneininchemotherapywithplatinumbasedcytostatics
AT kizekrene anadsorptivetransfertechniquecoupledwithbrdickareactiontorevealtheimportanceofmetallothioneininchemotherapywithplatinumbasedcytostatics
AT krizkovasona adsorptivetransfertechniquecoupledwithbrdickareactiontorevealtheimportanceofmetallothioneininchemotherapywithplatinumbasedcytostatics
AT fabrikivo adsorptivetransfertechniquecoupledwithbrdickareactiontorevealtheimportanceofmetallothioneininchemotherapywithplatinumbasedcytostatics
AT huskadalibor adsorptivetransfertechniquecoupledwithbrdickareactiontorevealtheimportanceofmetallothioneininchemotherapywithplatinumbasedcytostatics
AT adamvojtech adsorptivetransfertechniquecoupledwithbrdickareactiontorevealtheimportanceofmetallothioneininchemotherapywithplatinumbasedcytostatics
AT babulapetr adsorptivetransfertechniquecoupledwithbrdickareactiontorevealtheimportanceofmetallothioneininchemotherapywithplatinumbasedcytostatics
AT hrabetajan adsorptivetransfertechniquecoupledwithbrdickareactiontorevealtheimportanceofmetallothioneininchemotherapywithplatinumbasedcytostatics
AT eckschlagertomas adsorptivetransfertechniquecoupledwithbrdickareactiontorevealtheimportanceofmetallothioneininchemotherapywithplatinumbasedcytostatics
AT pochoppavel adsorptivetransfertechniquecoupledwithbrdickareactiontorevealtheimportanceofmetallothioneininchemotherapywithplatinumbasedcytostatics
AT darsovadenisa adsorptivetransfertechniquecoupledwithbrdickareactiontorevealtheimportanceofmetallothioneininchemotherapywithplatinumbasedcytostatics
AT kukackajiri adsorptivetransfertechniquecoupledwithbrdickareactiontorevealtheimportanceofmetallothioneininchemotherapywithplatinumbasedcytostatics
AT prusarichard adsorptivetransfertechniquecoupledwithbrdickareactiontorevealtheimportanceofmetallothioneininchemotherapywithplatinumbasedcytostatics
AT trnkovalibuse adsorptivetransfertechniquecoupledwithbrdickareactiontorevealtheimportanceofmetallothioneininchemotherapywithplatinumbasedcytostatics
AT kizekrene adsorptivetransfertechniquecoupledwithbrdickareactiontorevealtheimportanceofmetallothioneininchemotherapywithplatinumbasedcytostatics