Constitutive Expression of Thermobifida fusca Thermostable Acetylxylan Esterase Gene in Pichia pastoris

A gene encoding the thermostable acetylxylan esterase (AXE) in Thermobifida fusca NTU22 was amplified by PCR, sequenced and cloned into the Pichia pastoris X-33 host strain using the vector pGAPZαA, allowing constitutive expression and secretion of the protein. Recombinant expression resulted in hig...

Descripción completa

Detalles Bibliográficos
Autores principales: Yang, Chao-Hsun, Lin, Kun-I, Chen, Gen-Hung, Chen, Yu-Fen, Chen, Cheng-Yu, Chen, Wei-Lin, Huang, Yu-Chun
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3100850/
https://www.ncbi.nlm.nih.gov/pubmed/21614198
http://dx.doi.org/10.3390/ijms11125143

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3100850/
https://www.ncbi.nlm.nih.gov/pubmed/21614198
http://dx.doi.org/10.3390/ijms11125143

Ejemplares similares