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Constitutive Expression of Thermobifida fusca Thermostable Acetylxylan Esterase Gene in Pichia pastoris

A gene encoding the thermostable acetylxylan esterase (AXE) in Thermobifida fusca NTU22 was amplified by PCR, sequenced and cloned into the Pichia pastoris X-33 host strain using the vector pGAPZαA, allowing constitutive expression and secretion of the protein. Recombinant expression resulted in hig...

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Detalles Bibliográficos
Autores principales:	Yang, Chao-Hsun, Lin, Kun-I, Chen, Gen-Hung, Chen, Yu-Fen, Chen, Cheng-Yu, Chen, Wei-Lin, Huang, Yu-Chun
Formato:	Texto
Lenguaje:	English
Publicado:	Molecular Diversity Preservation International (MDPI) 2010
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3100850/ https://www.ncbi.nlm.nih.gov/pubmed/21614198 http://dx.doi.org/10.3390/ijms11125143

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