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Clinicopathological Significance of Loss of ARID1A Immunoreactivity in Ovarian Clear Cell Carcinoma

Recent genome-wide analysis has demonstrated that somatic mutations in ARID1A (BAF250) are the most common molecular genetic changes in ovarian clear cell carcinoma (OCCC). ARID1A mutations, which occur in approximately half of OCCC cases, lead to deletion of the encoded protein and inactivation of...

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Autores principales: Maeda, Daichi, Mao, Tsui-Lien, Fukayama, Masashi, Nakagawa, Shunsuke, Yano, Tetsu, Taketani, Yuji, Shih, Ie-Ming
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3100854/
https://www.ncbi.nlm.nih.gov/pubmed/21614196
http://dx.doi.org/10.3390/ijms11125120
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author Maeda, Daichi
Mao, Tsui-Lien
Fukayama, Masashi
Nakagawa, Shunsuke
Yano, Tetsu
Taketani, Yuji
Shih, Ie-Ming
author_facet Maeda, Daichi
Mao, Tsui-Lien
Fukayama, Masashi
Nakagawa, Shunsuke
Yano, Tetsu
Taketani, Yuji
Shih, Ie-Ming
author_sort Maeda, Daichi
collection PubMed
description Recent genome-wide analysis has demonstrated that somatic mutations in ARID1A (BAF250) are the most common molecular genetic changes in ovarian clear cell carcinoma (OCCC). ARID1A mutations, which occur in approximately half of OCCC cases, lead to deletion of the encoded protein and inactivation of the putative tumor suppressor. In this study, we determined the significance of loss of ARID1A immunoreactivity with respect to several clinicopathological features in a total of 149 OCCCs. First, we demonstrated that ARID1A immunohistochemistry showed concordance with the mutational status in 91% of cases with 100% sensitivity and 66% specificity. Specifically, among 12 OCCC cases for which ARIDA mutational status was known, ARIDIA immunoreactivity was undetectable in all 9 cases harboring ARID1A mutations and was undetectable in one of 3 cases with wild-type ARID1A. With respect to the entire cohort, ARID1A immunoreactivity was undetectable in 88 (59%) of 149 OCCCs. There was no significant difference between ARID1A negative and positive cases in terms of histopathologic features, age, clinical stage, or overall survival. In conclusion, this study provides further evidence that mutations in ARID1A resulted in loss of ARID1A protein expression in OCCC, although no significant differences between ARID1A positive and negative cases were observed with respect to any clinicopathological features examined.
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spelling pubmed-31008542011-05-25 Clinicopathological Significance of Loss of ARID1A Immunoreactivity in Ovarian Clear Cell Carcinoma Maeda, Daichi Mao, Tsui-Lien Fukayama, Masashi Nakagawa, Shunsuke Yano, Tetsu Taketani, Yuji Shih, Ie-Ming Int J Mol Sci Article Recent genome-wide analysis has demonstrated that somatic mutations in ARID1A (BAF250) are the most common molecular genetic changes in ovarian clear cell carcinoma (OCCC). ARID1A mutations, which occur in approximately half of OCCC cases, lead to deletion of the encoded protein and inactivation of the putative tumor suppressor. In this study, we determined the significance of loss of ARID1A immunoreactivity with respect to several clinicopathological features in a total of 149 OCCCs. First, we demonstrated that ARID1A immunohistochemistry showed concordance with the mutational status in 91% of cases with 100% sensitivity and 66% specificity. Specifically, among 12 OCCC cases for which ARIDA mutational status was known, ARIDIA immunoreactivity was undetectable in all 9 cases harboring ARID1A mutations and was undetectable in one of 3 cases with wild-type ARID1A. With respect to the entire cohort, ARID1A immunoreactivity was undetectable in 88 (59%) of 149 OCCCs. There was no significant difference between ARID1A negative and positive cases in terms of histopathologic features, age, clinical stage, or overall survival. In conclusion, this study provides further evidence that mutations in ARID1A resulted in loss of ARID1A protein expression in OCCC, although no significant differences between ARID1A positive and negative cases were observed with respect to any clinicopathological features examined. Molecular Diversity Preservation International (MDPI) 2010-12-13 /pmc/articles/PMC3100854/ /pubmed/21614196 http://dx.doi.org/10.3390/ijms11125120 Text en © 2010 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Maeda, Daichi
Mao, Tsui-Lien
Fukayama, Masashi
Nakagawa, Shunsuke
Yano, Tetsu
Taketani, Yuji
Shih, Ie-Ming
Clinicopathological Significance of Loss of ARID1A Immunoreactivity in Ovarian Clear Cell Carcinoma
title Clinicopathological Significance of Loss of ARID1A Immunoreactivity in Ovarian Clear Cell Carcinoma
title_full Clinicopathological Significance of Loss of ARID1A Immunoreactivity in Ovarian Clear Cell Carcinoma
title_fullStr Clinicopathological Significance of Loss of ARID1A Immunoreactivity in Ovarian Clear Cell Carcinoma
title_full_unstemmed Clinicopathological Significance of Loss of ARID1A Immunoreactivity in Ovarian Clear Cell Carcinoma
title_short Clinicopathological Significance of Loss of ARID1A Immunoreactivity in Ovarian Clear Cell Carcinoma
title_sort clinicopathological significance of loss of arid1a immunoreactivity in ovarian clear cell carcinoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3100854/
https://www.ncbi.nlm.nih.gov/pubmed/21614196
http://dx.doi.org/10.3390/ijms11125120
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