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Functional investigation of grass carp reovirus nonstructural protein NS80

BACKGROUND: Grass Carp Reovirus (GCRV), a highly virulent agent of aquatic animals, has an eleven segmented dsRNA genome encased in a multilayered capsid shell, which encodes twelve proteins including seven structural proteins (VP1-VP7), and five nonstructural proteins (NS80, NS38, NS31, NS26, and N...

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Autores principales: Cai, Lin, Sun, Xiaoyun, Shao, Ling, Fang, Qin
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3101161/
https://www.ncbi.nlm.nih.gov/pubmed/21489306
http://dx.doi.org/10.1186/1743-422X-8-168
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author Cai, Lin
Sun, Xiaoyun
Shao, Ling
Fang, Qin
author_facet Cai, Lin
Sun, Xiaoyun
Shao, Ling
Fang, Qin
author_sort Cai, Lin
collection PubMed
description BACKGROUND: Grass Carp Reovirus (GCRV), a highly virulent agent of aquatic animals, has an eleven segmented dsRNA genome encased in a multilayered capsid shell, which encodes twelve proteins including seven structural proteins (VP1-VP7), and five nonstructural proteins (NS80, NS38, NS31, NS26, and NS16). It has been suggested that the protein NS80 plays an important role in the viral replication cycle that is similar to that of its homologous protein μNS in the genus of Orthoreovirus. RESULTS: As a step to understanding the basis of the part played by NS80 in GCRV replication and particle assembly, we used the yeast two-hybrid (Y2H) system to identify NS80 interactions with proteins NS38, VP4, and VP6 as well as NS80 and NS38 self-interactions, while no interactions appeared in the four protein pairs NS38-VP4, NS38-VP6, VP4-VP4, and VP4-VP6. Bioinformatic analyses of NS80 with its corresponding proteins were performed with all currently available homologous protein sequences in ARVs (avian reoviruses) and MRVs (mammalian reoviruses) to predict further potential functional domains of NS80 that are related to VFLS (viral factory-like structures) formation and other roles in viral replication. Two conserved regions spanning from aa (amino acid) residues of 388 to 433, and 562 to 580 were discovered in this study. The second conserved region with corresponding conserved residues Tyr565, His569, Cys571, Asn573, and Glu576 located between the two coiled-coils regions (aa ~513-550 and aa ~615-690) in carboxyl-proximal terminus were supposed to be essential to form VFLS, so that aa residues ranging from 513 to 742 of NS80 was inferred to be the smallest region that is necessary for forming VFLS. The function of the first conserved region including Ala395, Gly419, Asp421, Pro422, Leu438, and Leu443 residues is unclear, but one-third of the amino-terminal region might be species specific, dominating interactions with other viral components. CONCLUSIONS: Our results in this study together with those from previous investigations indicate the protein NS80 might play a central role in VFLS formation and viral components recruitment in GCRV particle assembly, similar to the μNS protein in ARVs and MRVs.
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spelling pubmed-31011612011-05-25 Functional investigation of grass carp reovirus nonstructural protein NS80 Cai, Lin Sun, Xiaoyun Shao, Ling Fang, Qin Virol J Research BACKGROUND: Grass Carp Reovirus (GCRV), a highly virulent agent of aquatic animals, has an eleven segmented dsRNA genome encased in a multilayered capsid shell, which encodes twelve proteins including seven structural proteins (VP1-VP7), and five nonstructural proteins (NS80, NS38, NS31, NS26, and NS16). It has been suggested that the protein NS80 plays an important role in the viral replication cycle that is similar to that of its homologous protein μNS in the genus of Orthoreovirus. RESULTS: As a step to understanding the basis of the part played by NS80 in GCRV replication and particle assembly, we used the yeast two-hybrid (Y2H) system to identify NS80 interactions with proteins NS38, VP4, and VP6 as well as NS80 and NS38 self-interactions, while no interactions appeared in the four protein pairs NS38-VP4, NS38-VP6, VP4-VP4, and VP4-VP6. Bioinformatic analyses of NS80 with its corresponding proteins were performed with all currently available homologous protein sequences in ARVs (avian reoviruses) and MRVs (mammalian reoviruses) to predict further potential functional domains of NS80 that are related to VFLS (viral factory-like structures) formation and other roles in viral replication. Two conserved regions spanning from aa (amino acid) residues of 388 to 433, and 562 to 580 were discovered in this study. The second conserved region with corresponding conserved residues Tyr565, His569, Cys571, Asn573, and Glu576 located between the two coiled-coils regions (aa ~513-550 and aa ~615-690) in carboxyl-proximal terminus were supposed to be essential to form VFLS, so that aa residues ranging from 513 to 742 of NS80 was inferred to be the smallest region that is necessary for forming VFLS. The function of the first conserved region including Ala395, Gly419, Asp421, Pro422, Leu438, and Leu443 residues is unclear, but one-third of the amino-terminal region might be species specific, dominating interactions with other viral components. CONCLUSIONS: Our results in this study together with those from previous investigations indicate the protein NS80 might play a central role in VFLS formation and viral components recruitment in GCRV particle assembly, similar to the μNS protein in ARVs and MRVs. BioMed Central 2011-04-14 /pmc/articles/PMC3101161/ /pubmed/21489306 http://dx.doi.org/10.1186/1743-422X-8-168 Text en Copyright ©2011 Cai et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Cai, Lin
Sun, Xiaoyun
Shao, Ling
Fang, Qin
Functional investigation of grass carp reovirus nonstructural protein NS80
title Functional investigation of grass carp reovirus nonstructural protein NS80
title_full Functional investigation of grass carp reovirus nonstructural protein NS80
title_fullStr Functional investigation of grass carp reovirus nonstructural protein NS80
title_full_unstemmed Functional investigation of grass carp reovirus nonstructural protein NS80
title_short Functional investigation of grass carp reovirus nonstructural protein NS80
title_sort functional investigation of grass carp reovirus nonstructural protein ns80
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3101161/
https://www.ncbi.nlm.nih.gov/pubmed/21489306
http://dx.doi.org/10.1186/1743-422X-8-168
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