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ASPM and microcephalin expression in epithelial ovarian cancer correlates with tumour grade and survival
BACKGROUND: The clinico-pathological and molecular heterogeneity of epithelial ovarian cancer (EOC) complicates its early diagnosis and successful treatment. Highly aneuploid tumours and the presence of ascitic fluids are hallmarks of EOC. Two microcephaly-associated proteins, abnormal spindle-like...
Autores principales: | , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Nature Publishing Group
2011
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3101901/ https://www.ncbi.nlm.nih.gov/pubmed/21505456 http://dx.doi.org/10.1038/bjc.2011.117 |
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author | Brüning-Richardson, A Bond, J Alsiary, R Richardson, J Cairns, D A McCormack, L Hutson, R Burns, P Wilkinson, N Hall, G D Morrison, E E Bell, S M |
author_facet | Brüning-Richardson, A Bond, J Alsiary, R Richardson, J Cairns, D A McCormack, L Hutson, R Burns, P Wilkinson, N Hall, G D Morrison, E E Bell, S M |
author_sort | Brüning-Richardson, A |
collection | PubMed |
description | BACKGROUND: The clinico-pathological and molecular heterogeneity of epithelial ovarian cancer (EOC) complicates its early diagnosis and successful treatment. Highly aneuploid tumours and the presence of ascitic fluids are hallmarks of EOC. Two microcephaly-associated proteins, abnormal spindle-like microcephaly-associated protein (ASPM) and microcephalin, are involved in mitosis and DNA damage repair. Their expression is deregulated at the RNA level in EOC. Here, ASPM and microcephalin protein expression in primary cultures established from the ascites of patients with EOC was determined and correlated with clinical data to assess their suitability as biomarkers. METHODS: Five established ovarian cancer cell lines, cells derived from two benign ovarian ascites samples and 40 primary cultures of EOC derived from ovarian ascites samples were analysed by protein slot blotting and/or immunofluorescence to determine ASPM and microcephalin protein levels and their cellular localisation. Results were correlated with clinico-pathological data. RESULTS: A statistically significant correlation was identified for ASPM localisation and tumour grade, with high levels of cytoplasmic ASPM correlating with grade 1 tumours. Conversely, cytoplasmic microcephalin was only identified in high-grade tumours. Furthermore, low levels of nuclear microcephalin correlated with reduced patient survival. CONCLUSION: Our results suggest that ASPM and microcephalin have the potential to be biomarkers in ovarian cancer. |
format | Text |
id | pubmed-3101901 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-31019012012-05-10 ASPM and microcephalin expression in epithelial ovarian cancer correlates with tumour grade and survival Brüning-Richardson, A Bond, J Alsiary, R Richardson, J Cairns, D A McCormack, L Hutson, R Burns, P Wilkinson, N Hall, G D Morrison, E E Bell, S M Br J Cancer Molecular Diagnostics BACKGROUND: The clinico-pathological and molecular heterogeneity of epithelial ovarian cancer (EOC) complicates its early diagnosis and successful treatment. Highly aneuploid tumours and the presence of ascitic fluids are hallmarks of EOC. Two microcephaly-associated proteins, abnormal spindle-like microcephaly-associated protein (ASPM) and microcephalin, are involved in mitosis and DNA damage repair. Their expression is deregulated at the RNA level in EOC. Here, ASPM and microcephalin protein expression in primary cultures established from the ascites of patients with EOC was determined and correlated with clinical data to assess their suitability as biomarkers. METHODS: Five established ovarian cancer cell lines, cells derived from two benign ovarian ascites samples and 40 primary cultures of EOC derived from ovarian ascites samples were analysed by protein slot blotting and/or immunofluorescence to determine ASPM and microcephalin protein levels and their cellular localisation. Results were correlated with clinico-pathological data. RESULTS: A statistically significant correlation was identified for ASPM localisation and tumour grade, with high levels of cytoplasmic ASPM correlating with grade 1 tumours. Conversely, cytoplasmic microcephalin was only identified in high-grade tumours. Furthermore, low levels of nuclear microcephalin correlated with reduced patient survival. CONCLUSION: Our results suggest that ASPM and microcephalin have the potential to be biomarkers in ovarian cancer. Nature Publishing Group 2011-05-10 2011-04-19 /pmc/articles/PMC3101901/ /pubmed/21505456 http://dx.doi.org/10.1038/bjc.2011.117 Text en Copyright © 2011 Cancer Research UK https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Molecular Diagnostics Brüning-Richardson, A Bond, J Alsiary, R Richardson, J Cairns, D A McCormack, L Hutson, R Burns, P Wilkinson, N Hall, G D Morrison, E E Bell, S M ASPM and microcephalin expression in epithelial ovarian cancer correlates with tumour grade and survival |
title | ASPM and microcephalin expression in epithelial ovarian cancer correlates with tumour grade and survival |
title_full | ASPM and microcephalin expression in epithelial ovarian cancer correlates with tumour grade and survival |
title_fullStr | ASPM and microcephalin expression in epithelial ovarian cancer correlates with tumour grade and survival |
title_full_unstemmed | ASPM and microcephalin expression in epithelial ovarian cancer correlates with tumour grade and survival |
title_short | ASPM and microcephalin expression in epithelial ovarian cancer correlates with tumour grade and survival |
title_sort | aspm and microcephalin expression in epithelial ovarian cancer correlates with tumour grade and survival |
topic | Molecular Diagnostics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3101901/ https://www.ncbi.nlm.nih.gov/pubmed/21505456 http://dx.doi.org/10.1038/bjc.2011.117 |
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