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Rapid identification of Aspergillus fumigatus within the section Fumigati

BACKGROUND: New fungal species that are morphologically similar to Aspergillus fumigatus were recently described and included in section Fumigati. Misidentification of such fungal species, particularly of the human pathogens, Aspergillus lentulus, Neosartorya fischeri, Neosartorya hiratsukae, Neosar...

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Autores principales: Serrano, Rita, Gusmão, Leonor, Amorim, António, Araujo, Ricardo
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3102036/
https://www.ncbi.nlm.nih.gov/pubmed/21510879
http://dx.doi.org/10.1186/1471-2180-11-82
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author Serrano, Rita
Gusmão, Leonor
Amorim, António
Araujo, Ricardo
author_facet Serrano, Rita
Gusmão, Leonor
Amorim, António
Araujo, Ricardo
author_sort Serrano, Rita
collection PubMed
description BACKGROUND: New fungal species that are morphologically similar to Aspergillus fumigatus were recently described and included in section Fumigati. Misidentification of such fungal species, particularly of the human pathogens, Aspergillus lentulus, Neosartorya fischeri, Neosartorya hiratsukae, Neosartorya pseudofischeri and Neosartorya udagawae, has been increasingly reported by numerous clinical labs. Nevertheless, A. fumigatus still accounts for more than 90% of all invasive aspergillosis cases. The purpose of the present study was to develop a rapid method for the molecular identification of A. fumigatus to distinguish it from other species within the section Fumigati. RESULTS: A multiplex PCR was developed using prior information based on β-tubulin (βtub) and rodlet A (rodA) partial gene sequences. PCR amplification of βtub and rodA fragments resulted in a distinctive electrophoretic pattern in A. fumigatus and N. udagawae. The polymorphisms found in the smallest amplified sequence of βtub (153 bp) and rodA (103 bp) genes were then compared among and within species of this taxonomic section. βtub was able to differentiate among 13 individual species and two groups of species that included the pathogenic fungus A. lentulus. A more limited number of sequences were available for rodA; nevertheless, we were able to distinguish Aspergillus viridinutans, N. hiratsukae and N. udagawae. CONCLUSIONS: The assay described in the present study proved to be specific and highly reproducible, representing a fast and economic way of targeting molecular identification of the relevant mould, A. fumigatus, in clinical laboratories.
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spelling pubmed-31020362011-05-26 Rapid identification of Aspergillus fumigatus within the section Fumigati Serrano, Rita Gusmão, Leonor Amorim, António Araujo, Ricardo BMC Microbiol Methodology Article BACKGROUND: New fungal species that are morphologically similar to Aspergillus fumigatus were recently described and included in section Fumigati. Misidentification of such fungal species, particularly of the human pathogens, Aspergillus lentulus, Neosartorya fischeri, Neosartorya hiratsukae, Neosartorya pseudofischeri and Neosartorya udagawae, has been increasingly reported by numerous clinical labs. Nevertheless, A. fumigatus still accounts for more than 90% of all invasive aspergillosis cases. The purpose of the present study was to develop a rapid method for the molecular identification of A. fumigatus to distinguish it from other species within the section Fumigati. RESULTS: A multiplex PCR was developed using prior information based on β-tubulin (βtub) and rodlet A (rodA) partial gene sequences. PCR amplification of βtub and rodA fragments resulted in a distinctive electrophoretic pattern in A. fumigatus and N. udagawae. The polymorphisms found in the smallest amplified sequence of βtub (153 bp) and rodA (103 bp) genes were then compared among and within species of this taxonomic section. βtub was able to differentiate among 13 individual species and two groups of species that included the pathogenic fungus A. lentulus. A more limited number of sequences were available for rodA; nevertheless, we were able to distinguish Aspergillus viridinutans, N. hiratsukae and N. udagawae. CONCLUSIONS: The assay described in the present study proved to be specific and highly reproducible, representing a fast and economic way of targeting molecular identification of the relevant mould, A. fumigatus, in clinical laboratories. BioMed Central 2011-04-21 /pmc/articles/PMC3102036/ /pubmed/21510879 http://dx.doi.org/10.1186/1471-2180-11-82 Text en Copyright ©2011 Serrano et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology Article
Serrano, Rita
Gusmão, Leonor
Amorim, António
Araujo, Ricardo
Rapid identification of Aspergillus fumigatus within the section Fumigati
title Rapid identification of Aspergillus fumigatus within the section Fumigati
title_full Rapid identification of Aspergillus fumigatus within the section Fumigati
title_fullStr Rapid identification of Aspergillus fumigatus within the section Fumigati
title_full_unstemmed Rapid identification of Aspergillus fumigatus within the section Fumigati
title_short Rapid identification of Aspergillus fumigatus within the section Fumigati
title_sort rapid identification of aspergillus fumigatus within the section fumigati
topic Methodology Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3102036/
https://www.ncbi.nlm.nih.gov/pubmed/21510879
http://dx.doi.org/10.1186/1471-2180-11-82
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