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Competitive Binding to Cuprous Ions of Protein and BCA in the Bicinchoninic Acid Protein Assay
Although Bicinchoninic acid (BCA) has been widely used to determine protein concentration, the mechanism of interaction between protein, copper ion and BCA in this assay is still not well known. Using the Micro BCA protein assay kit (Pierce Company), we measured the absorbance at 562 nm of BSA solut...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Bentham Open
2010
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3102443/ https://www.ncbi.nlm.nih.gov/pubmed/21625379 http://dx.doi.org/10.2174/1874120701004010271 |