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Nuclear localization of the meiosis-specific transcription factor Ndt80 is regulated by the pachytene checkpoint

In budding yeast, the Ndt80 protein is a meiosis-specific transcription factor that is essential for the exit of pachytene and progression into nuclear divisions and spore formation. The pachytene checkpoint responds to defects in meiotic recombination and chromosome synapsis and negatively regulate...

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Autores principales: Wang, Ying, Chang, Chun-Yu, Wu, Jing-Fen, Tung, Kuei-Shu
Formato: Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3103403/
https://www.ncbi.nlm.nih.gov/pubmed/21471004
http://dx.doi.org/10.1091/mbc.E10-12-1011
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author Wang, Ying
Chang, Chun-Yu
Wu, Jing-Fen
Tung, Kuei-Shu
author_facet Wang, Ying
Chang, Chun-Yu
Wu, Jing-Fen
Tung, Kuei-Shu
author_sort Wang, Ying
collection PubMed
description In budding yeast, the Ndt80 protein is a meiosis-specific transcription factor that is essential for the exit of pachytene and progression into nuclear divisions and spore formation. The pachytene checkpoint responds to defects in meiotic recombination and chromosome synapsis and negatively regulates the activity of Ndt80. The activity of Ndt80 was suggested to be regulated at both transcriptional and posttranslational levels; however, the mechanism for posttranslational regulation of Ndt80 was unclear. From a study of ndt80 in-frame deletion mutations, we have identified a dominant mutation NDT80-bc, which is able to completely bypass the pachytene checkpoint. The NDT80-bc mutation relieves the checkpoint-mediated arrest of the zip1, dmc1, and hop2 mutants, producing spores with low viability. The NDT80-bc mutant provides direct evidence for the posttranslational control of Ndt80 activity. Furthermore, the data presented show that Ndt80 is retained in cytoplasm in the zip1 mutant, whereas Ndt80-bc is found in the nucleus. We propose that the nuclear localization of Ndt80 is regulated by the pachytene checkpoint through a cytoplasmic anchor mechanism.
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spelling pubmed-31034032011-08-16 Nuclear localization of the meiosis-specific transcription factor Ndt80 is regulated by the pachytene checkpoint Wang, Ying Chang, Chun-Yu Wu, Jing-Fen Tung, Kuei-Shu Mol Biol Cell Articles In budding yeast, the Ndt80 protein is a meiosis-specific transcription factor that is essential for the exit of pachytene and progression into nuclear divisions and spore formation. The pachytene checkpoint responds to defects in meiotic recombination and chromosome synapsis and negatively regulates the activity of Ndt80. The activity of Ndt80 was suggested to be regulated at both transcriptional and posttranslational levels; however, the mechanism for posttranslational regulation of Ndt80 was unclear. From a study of ndt80 in-frame deletion mutations, we have identified a dominant mutation NDT80-bc, which is able to completely bypass the pachytene checkpoint. The NDT80-bc mutation relieves the checkpoint-mediated arrest of the zip1, dmc1, and hop2 mutants, producing spores with low viability. The NDT80-bc mutant provides direct evidence for the posttranslational control of Ndt80 activity. Furthermore, the data presented show that Ndt80 is retained in cytoplasm in the zip1 mutant, whereas Ndt80-bc is found in the nucleus. We propose that the nuclear localization of Ndt80 is regulated by the pachytene checkpoint through a cytoplasmic anchor mechanism. The American Society for Cell Biology 2011-06-01 /pmc/articles/PMC3103403/ /pubmed/21471004 http://dx.doi.org/10.1091/mbc.E10-12-1011 Text en © 2011 Wang et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB”, “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Wang, Ying
Chang, Chun-Yu
Wu, Jing-Fen
Tung, Kuei-Shu
Nuclear localization of the meiosis-specific transcription factor Ndt80 is regulated by the pachytene checkpoint
title Nuclear localization of the meiosis-specific transcription factor Ndt80 is regulated by the pachytene checkpoint
title_full Nuclear localization of the meiosis-specific transcription factor Ndt80 is regulated by the pachytene checkpoint
title_fullStr Nuclear localization of the meiosis-specific transcription factor Ndt80 is regulated by the pachytene checkpoint
title_full_unstemmed Nuclear localization of the meiosis-specific transcription factor Ndt80 is regulated by the pachytene checkpoint
title_short Nuclear localization of the meiosis-specific transcription factor Ndt80 is regulated by the pachytene checkpoint
title_sort nuclear localization of the meiosis-specific transcription factor ndt80 is regulated by the pachytene checkpoint
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3103403/
https://www.ncbi.nlm.nih.gov/pubmed/21471004
http://dx.doi.org/10.1091/mbc.E10-12-1011
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