Cargando…

A quantitative real-time RT-PCR assay for mature C. albicans biofilms

BACKGROUND: Fungal biofilms are more resistant to anti-fungal drugs than organisms in planktonic form. Traditionally, susceptibility of biofilms to anti-fungal agents has been measured using the 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxyanilide (XTT) assay, which measures the...

Descripción completa

Detalles Bibliográficos
Autores principales:	Xie, Zhihong, Thompson, Angela, Kashleva, Helena, Dongari-Bagtzoglou, Anna
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2011
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3103424/ https://www.ncbi.nlm.nih.gov/pubmed/21548962 http://dx.doi.org/10.1186/1471-2180-11-93

Ejemplares similares

Characterization of Mucosal Candida albicans Biofilms
por: Dongari-Bagtzoglou, Anna, et al.
Publicado: (2009)

Role of Bcr1-Activated Genes Hwp1 and Hyr1 in Candida Albicans Oral Mucosal Biofilms and Neutrophil Evasion
por: Dwivedi, Prabhat, et al.
Publicado: (2011)

Streptococcus gordonii glucosyltransferase promotes biofilm interactions with Candida albicans
por: Ricker, Austin, et al.
Publicado: (2014)

Host Cell Invasion and Virulence Mediated by Candida albicans Ssa1
por: Sun, Jianing N., et al.
Publicado: (2010)

A one step real-time RT-PCR assay for the quantitation of Wheat yellow mosaic virus (WYMV)
por: Liu, Wenwen, et al.
Publicado: (2013)

Development of a real-time RT-PCR assay for the detection of pan-human parechoviruses
por: Lu, Huanhuan, et al.
Publicado: (2021)

Real-time RT-PCR assay to detect Granada virus and the related Massilia and Arrabida phleboviruses
por: Davó, Laura, et al.
Publicado: (2020)

Tipping the Balance: C. albicans Adaptation in Polymicrobial Environments
por: Ranjan, Amit, et al.
Publicado: (2018)

Ex vivo screening for immunodominant viral epitopes by quantitative real time polymerase chain reaction (qRT-PCR)
por: Provenzano, Maurizio, et al.
Publicado: (2003)

A TaqMan real-time PCR assay for the detection and quantitation of Plasmodium knowlesi
por: Divis, Paul CS, et al.
Publicado: (2010)

Comparative evaluation of a laboratory-developed real-time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus
por: Wong, Samson S. Y., et al.
Publicado: (2018)

Glucanase Induces Filamentation of the Fungal Pathogen Candida albicans
por: Xu, Hongbin, et al.
Publicado: (2013)

Role of glucosyltransferase R in biofilm interactions between Streptococcus oralis and Candida albicans
por: Souza, João Gabriel Silva, et al.
Publicado: (2020)

Development and evaluation of a SYBR Green real-time RT-PCR assay for detection of avian hepatitis E virus
por: Zhao, Qin, et al.
Publicado: (2015)

Two approaches for estimating the lower limit of quantitation (LLOQ) of microRNA levels assayed as exploratory biomarkers by RT-qPCR
por: Wolfinger, Russell D., et al.
Publicado: (2018)

Detection of bovine viral diarrhea virus genotype 1 in aerosol by a real time RT-PCR assay
por: Hou, Peili, et al.
Publicado: (2020)

Candida albicans induces mucosal bacterial dysbiosis that promotes invasive infection
por: Bertolini, Martinna, et al.
Publicado: (2019)

Development of one-step SYBR Green real-time RT-PCR for quantifying bovine viral diarrhea virus type-1 and its comparison with conventional RT-PCR
por: Zhang, Ni, et al.
Publicado: (2011)

Selection of optimal reference genes for normalization in quantitative RT-PCR
por: Chervoneva, Inna, et al.
Publicado: (2010)

Validation of reference genes for quantitative RT-PCR studies in porcine oocytes and preimplantation embryos
por: Kuijk, Ewart W, et al.
Publicado: (2007)

A multiplex one-tube nested real time RT-PCR assay for simultaneous detection of respiratory syncytial virus, human rhinovirus and human metapneumovirus
por: Feng, Zhi-shan, et al.
Publicado: (2018)

Insights From the Lactobacillus johnsonii Genome Suggest the Production of Metabolites With Antibiofilm Activity Against the Pathobiont Candida albicans
por: Vazquez-Munoz, Roberto, et al.
Publicado: (2022)

Applicability of RNA standards for evaluating RT-qPCR assays and platforms
por: Devonshire, Alison S, et al.
Publicado: (2011)

Development of a real-time quantitative PCR assay for detection of a stable genomic region of BK virus
por: Iwaki, Kosuke K, et al.
Publicado: (2010)

Biomarker discovery for colon cancer using a 761 gene RT-PCR assay
por: Clark-Langone, Kim M, et al.
Publicado: (2007)

Development of a reverse transcription droplet digital PCR (RT-ddPCR) assay for sensitive detection of simian immunodeficiency virus (SIV)
por: Long, Samuel, et al.
Publicado: (2021)

Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers
por: Balcells, Ingrid, et al.
Publicado: (2011)

Data-driven normalization strategies for high-throughput quantitative RT-PCR
por: Mar, Jessica C, et al.
Publicado: (2009)

BactQuant: An enhanced broad-coverage bacterial quantitative real-time PCR assay
por: Liu, Cindy M, et al.
Publicado: (2012)

FungiQuant: A broad-coverage fungal quantitative real-time PCR assay
por: Liu, Cindy M, et al.
Publicado: (2012)

A triplex quantitative real-time PCR assay for differential detection of human adenovirus serotypes 2, 3 and 7
por: Qiu, Fang-zhou, et al.
Publicado: (2018)

A quantitative RT-PCR platform for high-throughput expression profiling of 2500 rice transcription factors
por: Caldana, Camila, et al.
Publicado: (2007)

A plastome primer set for comprehensive quantitative real time RT-PCR analysis of Zea mays: a starter primer set for other Poaceae species
por: Sharpe, Richard M, et al.
Publicado: (2008)

Preamplification techniques for real-time RT-PCR analyses of endomyocardial biopsies
por: Noutsias, Michel, et al.
Publicado: (2008)

A SYBR Green RT-PCR assay in single tube to detect human and bovine noroviruses and control for inhibition
por: Scipioni, Alexandra, et al.
Publicado: (2008)

Validation and utilization of an internally controlled multiplex Real-time RT-PCR assay for simultaneous detection of enteroviruses and enterovirus A71 associated with hand foot and mouth disease
por: Thanh, Tran Tan, et al.
Publicado: (2015)

A duplex real-time PCR assay for the detection and quantification of avian reovirus and Mycoplasma synoviae
por: Huang, Li, et al.
Publicado: (2015)

Real-time RT-PCR high-resolution melting curve analysis and multiplex RT-PCR to detect and differentiate grapevine leafroll-associated virus 3 variant groups I, II, III and VI
por: Bester, Rachelle, et al.
Publicado: (2012)

Understanding Lactobacillus paracasei and Streptococcus oralis Biofilm Interactions through Agent-Based Modeling
por: Archambault, Linda, et al.
Publicado: (2021)

Identification of reliable reference genes for quantitative real-time PCR normalization in pitaya
por: Chen, Canbin, et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_vi1saqqr5c4pn0r5llj7b339n2