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Molecular and functional analyses of COPT/Ctr-type copper transporter-like gene family in rice
BACKGROUND: The copper (Cu) transporter (COPT/Ctr) gene family has an important role in the maintenance of Cu homeostasis in different species. The rice COPT-type gene family consists of seven members (COPT1 to COPT7). However, only two, COPT1 and COPT5, have been characterized for their functions i...
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2011
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3103425/ https://www.ncbi.nlm.nih.gov/pubmed/21510855 http://dx.doi.org/10.1186/1471-2229-11-69 |
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author | Yuan, Meng Li, Xianghua Xiao, Jinghua Wang, Shiping |
author_facet | Yuan, Meng Li, Xianghua Xiao, Jinghua Wang, Shiping |
author_sort | Yuan, Meng |
collection | PubMed |
description | BACKGROUND: The copper (Cu) transporter (COPT/Ctr) gene family has an important role in the maintenance of Cu homeostasis in different species. The rice COPT-type gene family consists of seven members (COPT1 to COPT7). However, only two, COPT1 and COPT5, have been characterized for their functions in Cu transport. RESULTS: Here we report the molecular and functional characterization of the other five members of the rice COPT gene family (COPT2, COPT3, COPT4, COPT6, and COPT7). All members of the rice COPT family have the conserved features of known COPT/Ctr-type Cu transporter genes. Among the proteins encoded by rice COPTs, COPT2, COPT3, and COPT4 physically interacted with COPT6, respectively, except for the known interaction between COPT1 and COPT5. COPT2, COPT3, or COPT4 cooperating with COPT6 mediated a high-affinity Cu uptake in the yeast Saccharomyces cerevisiae mutant that lacked the functions of ScCtr1 and ScCtr3 for Cu uptake. COPT7 alone could mediate a high-affinity Cu uptake in the yeast mutant. None of the seven COPTs alone or in cooperation could complement the phenotypes of S. cerevisiae mutants that lacked the transporter genes either for iron uptake or for zinc uptake. However, these COPT genes, which showed different tissue-specific expression patterns and Cu level-regulated expression patterns, were also transcriptionally influenced by deficiency of iron, manganese, or zinc. CONCLUSION: These results suggest that COPT2, COPT3, and COPT4 may cooperate with COPT6, respectively, and COPT7 acts alone for Cu transport in different rice tissues. The endogenous concentrations of iron, manganese, or zinc may influence Cu homeostasis by influencing the expression of COPTs in rice. |
format | Text |
id | pubmed-3103425 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-31034252011-05-28 Molecular and functional analyses of COPT/Ctr-type copper transporter-like gene family in rice Yuan, Meng Li, Xianghua Xiao, Jinghua Wang, Shiping BMC Plant Biol Research Article BACKGROUND: The copper (Cu) transporter (COPT/Ctr) gene family has an important role in the maintenance of Cu homeostasis in different species. The rice COPT-type gene family consists of seven members (COPT1 to COPT7). However, only two, COPT1 and COPT5, have been characterized for their functions in Cu transport. RESULTS: Here we report the molecular and functional characterization of the other five members of the rice COPT gene family (COPT2, COPT3, COPT4, COPT6, and COPT7). All members of the rice COPT family have the conserved features of known COPT/Ctr-type Cu transporter genes. Among the proteins encoded by rice COPTs, COPT2, COPT3, and COPT4 physically interacted with COPT6, respectively, except for the known interaction between COPT1 and COPT5. COPT2, COPT3, or COPT4 cooperating with COPT6 mediated a high-affinity Cu uptake in the yeast Saccharomyces cerevisiae mutant that lacked the functions of ScCtr1 and ScCtr3 for Cu uptake. COPT7 alone could mediate a high-affinity Cu uptake in the yeast mutant. None of the seven COPTs alone or in cooperation could complement the phenotypes of S. cerevisiae mutants that lacked the transporter genes either for iron uptake or for zinc uptake. However, these COPT genes, which showed different tissue-specific expression patterns and Cu level-regulated expression patterns, were also transcriptionally influenced by deficiency of iron, manganese, or zinc. CONCLUSION: These results suggest that COPT2, COPT3, and COPT4 may cooperate with COPT6, respectively, and COPT7 acts alone for Cu transport in different rice tissues. The endogenous concentrations of iron, manganese, or zinc may influence Cu homeostasis by influencing the expression of COPTs in rice. BioMed Central 2011-04-21 /pmc/articles/PMC3103425/ /pubmed/21510855 http://dx.doi.org/10.1186/1471-2229-11-69 Text en Copyright ©2011 Yuan et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Yuan, Meng Li, Xianghua Xiao, Jinghua Wang, Shiping Molecular and functional analyses of COPT/Ctr-type copper transporter-like gene family in rice |
title | Molecular and functional analyses of COPT/Ctr-type copper transporter-like gene family in rice |
title_full | Molecular and functional analyses of COPT/Ctr-type copper transporter-like gene family in rice |
title_fullStr | Molecular and functional analyses of COPT/Ctr-type copper transporter-like gene family in rice |
title_full_unstemmed | Molecular and functional analyses of COPT/Ctr-type copper transporter-like gene family in rice |
title_short | Molecular and functional analyses of COPT/Ctr-type copper transporter-like gene family in rice |
title_sort | molecular and functional analyses of copt/ctr-type copper transporter-like gene family in rice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3103425/ https://www.ncbi.nlm.nih.gov/pubmed/21510855 http://dx.doi.org/10.1186/1471-2229-11-69 |
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