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Dynamic analysis of Ca(2+) level during bovine oocytes maturation and early embryonic development
Mammalian oocyte maturation and early embryo development processes are Ca(2+)-dependent. In this study, we used confocal microscopy to investigate the distribution pattern of Ca(2+) and its dynamic changes in the processes of bovine oocytes maturation, in vitro fertilization (IVF), parthenogenetic a...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Korean Society of Veterinary Science
2011
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3104167/ https://www.ncbi.nlm.nih.gov/pubmed/21586872 http://dx.doi.org/10.4142/jvs.2011.12.2.133 |
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author | Liang, Su Li Zhao, Qian Jun Li, Xiang Chen Jin, Ya Ping Wang, Yi Peng Su, Xiao Hua Guan, Wei Jun Ma, Yue Hui |
author_facet | Liang, Su Li Zhao, Qian Jun Li, Xiang Chen Jin, Ya Ping Wang, Yi Peng Su, Xiao Hua Guan, Wei Jun Ma, Yue Hui |
author_sort | Liang, Su Li |
collection | PubMed |
description | Mammalian oocyte maturation and early embryo development processes are Ca(2+)-dependent. In this study, we used confocal microscopy to investigate the distribution pattern of Ca(2+) and its dynamic changes in the processes of bovine oocytes maturation, in vitro fertilization (IVF), parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) embryo development. During the germinal vesicle (GV) and GV breakdown stage, Ca(2+) was distributed in the cortical ooplasm and throughout the oocytes from the MI to MII stage. In IVF embryos, Ca(2+) was distributed in the cortical ooplasm before the formation of the pronucleus. In 4-8 cell embryos and morulas, Ca(2+) was present throughout the blastomere. In PA embryos, Ca(2+) was distributed throughout the blastomere at 48 h, similar to in the 4-cell and 8-cell phase and the morula. At 6 h after activation, there was almost no distribution of Ca(2+) in the SCNT embryos. However, Ca(2+) was distributed in the donor nucleus at 10 h and it was distributed throughout the blastomere in the 2-8 cell embryos. In this study, Ca(2+) showed significant fluctuations with regularity of IVF and SCNT groups, but PA did not. Systematic investigation of the Ca(2+) location and distribution changes during oocyte maturation and early embryo development processes should facilitate a better understanding of the mechanisms involved in oocyte maturation, reconstructed embryo activation and development, ultimately improving the reconstructed embryo development rate. |
format | Text |
id | pubmed-3104167 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | The Korean Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-31041672011-06-09 Dynamic analysis of Ca(2+) level during bovine oocytes maturation and early embryonic development Liang, Su Li Zhao, Qian Jun Li, Xiang Chen Jin, Ya Ping Wang, Yi Peng Su, Xiao Hua Guan, Wei Jun Ma, Yue Hui J Vet Sci Original Article Mammalian oocyte maturation and early embryo development processes are Ca(2+)-dependent. In this study, we used confocal microscopy to investigate the distribution pattern of Ca(2+) and its dynamic changes in the processes of bovine oocytes maturation, in vitro fertilization (IVF), parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) embryo development. During the germinal vesicle (GV) and GV breakdown stage, Ca(2+) was distributed in the cortical ooplasm and throughout the oocytes from the MI to MII stage. In IVF embryos, Ca(2+) was distributed in the cortical ooplasm before the formation of the pronucleus. In 4-8 cell embryos and morulas, Ca(2+) was present throughout the blastomere. In PA embryos, Ca(2+) was distributed throughout the blastomere at 48 h, similar to in the 4-cell and 8-cell phase and the morula. At 6 h after activation, there was almost no distribution of Ca(2+) in the SCNT embryos. However, Ca(2+) was distributed in the donor nucleus at 10 h and it was distributed throughout the blastomere in the 2-8 cell embryos. In this study, Ca(2+) showed significant fluctuations with regularity of IVF and SCNT groups, but PA did not. Systematic investigation of the Ca(2+) location and distribution changes during oocyte maturation and early embryo development processes should facilitate a better understanding of the mechanisms involved in oocyte maturation, reconstructed embryo activation and development, ultimately improving the reconstructed embryo development rate. The Korean Society of Veterinary Science 2011-06 2011-05-19 /pmc/articles/PMC3104167/ /pubmed/21586872 http://dx.doi.org/10.4142/jvs.2011.12.2.133 Text en Copyright © 2011 The Korean Society of Veterinary Science https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Liang, Su Li Zhao, Qian Jun Li, Xiang Chen Jin, Ya Ping Wang, Yi Peng Su, Xiao Hua Guan, Wei Jun Ma, Yue Hui Dynamic analysis of Ca(2+) level during bovine oocytes maturation and early embryonic development |
title | Dynamic analysis of Ca(2+) level during bovine oocytes maturation and early embryonic development |
title_full | Dynamic analysis of Ca(2+) level during bovine oocytes maturation and early embryonic development |
title_fullStr | Dynamic analysis of Ca(2+) level during bovine oocytes maturation and early embryonic development |
title_full_unstemmed | Dynamic analysis of Ca(2+) level during bovine oocytes maturation and early embryonic development |
title_short | Dynamic analysis of Ca(2+) level during bovine oocytes maturation and early embryonic development |
title_sort | dynamic analysis of ca(2+) level during bovine oocytes maturation and early embryonic development |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3104167/ https://www.ncbi.nlm.nih.gov/pubmed/21586872 http://dx.doi.org/10.4142/jvs.2011.12.2.133 |
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