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Virulence factors in Escherichia coli isolated from calves with diarrhea in Vietnam
This study was conducted to determine the prevalence and characteristics of pathogenic Escherichia (E.) coli strains from diarrheic calves in Vietnam. A total of 345 E. coli isolates obtained from 322 diarrheic calves were subjected to PCR and multiplex PCR for detection of the f5, f41, f17, eae, st...
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Formato: | Texto |
Lenguaje: | English |
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The Korean Society of Veterinary Science
2011
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3104170/ https://www.ncbi.nlm.nih.gov/pubmed/21586875 http://dx.doi.org/10.4142/jvs.2011.12.2.159 |
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author | Nguyen, Tan Duc Vo, Thin Thanh Vu-Khac, Hung |
author_facet | Nguyen, Tan Duc Vo, Thin Thanh Vu-Khac, Hung |
author_sort | Nguyen, Tan Duc |
collection | PubMed |
description | This study was conducted to determine the prevalence and characteristics of pathogenic Escherichia (E.) coli strains from diarrheic calves in Vietnam. A total of 345 E. coli isolates obtained from 322 diarrheic calves were subjected to PCR and multiplex PCR for detection of the f5, f41, f17, eae, sta, lt, stx1, and stx2 genes. Of the 345 isolates, 108 (31.3%) carried at least one fimbrial gene. Of these 108 isolates, 50 carried genes for Shiga toxin and one possessed genes for both enterotoxin and Shiga toxin. The eae gene was found in 34 isolates (9.8%), 23 of which also carried stx genes. The Shiga toxin genes were detected in 177 isolates (51.3%) and the number of strains that carried stx1, stx2 and stx1/stx2 were 46, 73 and 58, respectively. Among 177 Shiga toxin-producing E. coli isolates, 89 carried the ehxA gene and 87 possessed the saa gene. Further characterization of the stx subtypes showed that among 104 stx1-positive isolates, 58 were the stx1c variant and 46 were the stx1 variant. Of the 131 stx2-positive strains, 48 were stx2, 48 were stx2c, 11 were stx2d, 17 were stx2g, and seven were stx2c/stx2g subtypes. The serogroups most prevalent among the 345 isolates were O15, O20, O103 and O157. |
format | Text |
id | pubmed-3104170 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | The Korean Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-31041702011-06-09 Virulence factors in Escherichia coli isolated from calves with diarrhea in Vietnam Nguyen, Tan Duc Vo, Thin Thanh Vu-Khac, Hung J Vet Sci Original Article This study was conducted to determine the prevalence and characteristics of pathogenic Escherichia (E.) coli strains from diarrheic calves in Vietnam. A total of 345 E. coli isolates obtained from 322 diarrheic calves were subjected to PCR and multiplex PCR for detection of the f5, f41, f17, eae, sta, lt, stx1, and stx2 genes. Of the 345 isolates, 108 (31.3%) carried at least one fimbrial gene. Of these 108 isolates, 50 carried genes for Shiga toxin and one possessed genes for both enterotoxin and Shiga toxin. The eae gene was found in 34 isolates (9.8%), 23 of which also carried stx genes. The Shiga toxin genes were detected in 177 isolates (51.3%) and the number of strains that carried stx1, stx2 and stx1/stx2 were 46, 73 and 58, respectively. Among 177 Shiga toxin-producing E. coli isolates, 89 carried the ehxA gene and 87 possessed the saa gene. Further characterization of the stx subtypes showed that among 104 stx1-positive isolates, 58 were the stx1c variant and 46 were the stx1 variant. Of the 131 stx2-positive strains, 48 were stx2, 48 were stx2c, 11 were stx2d, 17 were stx2g, and seven were stx2c/stx2g subtypes. The serogroups most prevalent among the 345 isolates were O15, O20, O103 and O157. The Korean Society of Veterinary Science 2011-06 2011-05-19 /pmc/articles/PMC3104170/ /pubmed/21586875 http://dx.doi.org/10.4142/jvs.2011.12.2.159 Text en Copyright © 2011 The Korean Society of Veterinary Science https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Nguyen, Tan Duc Vo, Thin Thanh Vu-Khac, Hung Virulence factors in Escherichia coli isolated from calves with diarrhea in Vietnam |
title | Virulence factors in Escherichia coli isolated from calves with diarrhea in Vietnam |
title_full | Virulence factors in Escherichia coli isolated from calves with diarrhea in Vietnam |
title_fullStr | Virulence factors in Escherichia coli isolated from calves with diarrhea in Vietnam |
title_full_unstemmed | Virulence factors in Escherichia coli isolated from calves with diarrhea in Vietnam |
title_short | Virulence factors in Escherichia coli isolated from calves with diarrhea in Vietnam |
title_sort | virulence factors in escherichia coli isolated from calves with diarrhea in vietnam |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3104170/ https://www.ncbi.nlm.nih.gov/pubmed/21586875 http://dx.doi.org/10.4142/jvs.2011.12.2.159 |
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