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Early and Late Postnatal Myocardial and Vascular Changes in a Protein Restriction Rat Model of Intrauterine Growth Restriction

Intrauterine growth restriction (IUGR) is a risk factor for cardiovascular disease in later life. Early structural and functional changes in the cardiovascular system after IUGR may contribute to its pathogenesis. We tested the hypothesis that IUGR leads to primary myocardial and vascular alteration...

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Autores principales: Menendez-Castro, Carlos, Fahlbusch, Fabian, Cordasic, Nada, Amann, Kerstin, Münzel, Kathrin, Plank, Christian, Wachtveitl, Rainer, Rascher, Wolfgang, Hilgers, Karl F., Hartner, Andrea
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3105022/
https://www.ncbi.nlm.nih.gov/pubmed/21655297
http://dx.doi.org/10.1371/journal.pone.0020369
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author Menendez-Castro, Carlos
Fahlbusch, Fabian
Cordasic, Nada
Amann, Kerstin
Münzel, Kathrin
Plank, Christian
Wachtveitl, Rainer
Rascher, Wolfgang
Hilgers, Karl F.
Hartner, Andrea
author_facet Menendez-Castro, Carlos
Fahlbusch, Fabian
Cordasic, Nada
Amann, Kerstin
Münzel, Kathrin
Plank, Christian
Wachtveitl, Rainer
Rascher, Wolfgang
Hilgers, Karl F.
Hartner, Andrea
author_sort Menendez-Castro, Carlos
collection PubMed
description Intrauterine growth restriction (IUGR) is a risk factor for cardiovascular disease in later life. Early structural and functional changes in the cardiovascular system after IUGR may contribute to its pathogenesis. We tested the hypothesis that IUGR leads to primary myocardial and vascular alterations before the onset of hypertension. A rat IUGR model of maternal protein restriction during gestation was used. Dams were fed low protein (LP; casein 8.4%) or isocaloric normal protein diet (NP; casein 17.2%). The offspring was reduced to six males per litter. Immunohistochemical and real-time PCR analyses were performed in myocardial and vascular tissue of neonates and animals at day 70 of life. In the aortas of newborn IUGR rats expression of connective tissue growth factor (CTGF) was induced 3.2-fold. At day 70 of life, the expression of collagen I was increased 5.6-fold in aortas of IUGR rats. In the hearts of neonate IUGR rats, cell proliferation was more prominent compared to controls. At day 70 the expression of osteopontin was induced 7.2-fold. A 3- to 7-fold increase in the expression of the profibrotic cytokines TGF-β and CTGF as well as of microfibrillar matrix molecules was observed. The myocardial expression and deposition of collagens was more prominent in IUGR animals compared to controls at day 70. In the low-protein diet model, IUGR leads to changes in the expression patterns of profibrotic genes and discrete structural abnormalities of vessels and hearts in adolescence, but, with the exception of CTGF, not as early as at the time of birth. Invasive and non-invasive blood pressure measurements confirmed that IUGR rats were normotensive at the time point investigated and that the changes observed occurred independently of an increased blood pressure. Hence, altered matrix composition of the vascular wall and the myocardium may predispose IUGR animals to cardiovascular disease later in life.
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spelling pubmed-31050222011-06-08 Early and Late Postnatal Myocardial and Vascular Changes in a Protein Restriction Rat Model of Intrauterine Growth Restriction Menendez-Castro, Carlos Fahlbusch, Fabian Cordasic, Nada Amann, Kerstin Münzel, Kathrin Plank, Christian Wachtveitl, Rainer Rascher, Wolfgang Hilgers, Karl F. Hartner, Andrea PLoS One Research Article Intrauterine growth restriction (IUGR) is a risk factor for cardiovascular disease in later life. Early structural and functional changes in the cardiovascular system after IUGR may contribute to its pathogenesis. We tested the hypothesis that IUGR leads to primary myocardial and vascular alterations before the onset of hypertension. A rat IUGR model of maternal protein restriction during gestation was used. Dams were fed low protein (LP; casein 8.4%) or isocaloric normal protein diet (NP; casein 17.2%). The offspring was reduced to six males per litter. Immunohistochemical and real-time PCR analyses were performed in myocardial and vascular tissue of neonates and animals at day 70 of life. In the aortas of newborn IUGR rats expression of connective tissue growth factor (CTGF) was induced 3.2-fold. At day 70 of life, the expression of collagen I was increased 5.6-fold in aortas of IUGR rats. In the hearts of neonate IUGR rats, cell proliferation was more prominent compared to controls. At day 70 the expression of osteopontin was induced 7.2-fold. A 3- to 7-fold increase in the expression of the profibrotic cytokines TGF-β and CTGF as well as of microfibrillar matrix molecules was observed. The myocardial expression and deposition of collagens was more prominent in IUGR animals compared to controls at day 70. In the low-protein diet model, IUGR leads to changes in the expression patterns of profibrotic genes and discrete structural abnormalities of vessels and hearts in adolescence, but, with the exception of CTGF, not as early as at the time of birth. Invasive and non-invasive blood pressure measurements confirmed that IUGR rats were normotensive at the time point investigated and that the changes observed occurred independently of an increased blood pressure. Hence, altered matrix composition of the vascular wall and the myocardium may predispose IUGR animals to cardiovascular disease later in life. Public Library of Science 2011-05-31 /pmc/articles/PMC3105022/ /pubmed/21655297 http://dx.doi.org/10.1371/journal.pone.0020369 Text en Menendez-Castro et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Menendez-Castro, Carlos
Fahlbusch, Fabian
Cordasic, Nada
Amann, Kerstin
Münzel, Kathrin
Plank, Christian
Wachtveitl, Rainer
Rascher, Wolfgang
Hilgers, Karl F.
Hartner, Andrea
Early and Late Postnatal Myocardial and Vascular Changes in a Protein Restriction Rat Model of Intrauterine Growth Restriction
title Early and Late Postnatal Myocardial and Vascular Changes in a Protein Restriction Rat Model of Intrauterine Growth Restriction
title_full Early and Late Postnatal Myocardial and Vascular Changes in a Protein Restriction Rat Model of Intrauterine Growth Restriction
title_fullStr Early and Late Postnatal Myocardial and Vascular Changes in a Protein Restriction Rat Model of Intrauterine Growth Restriction
title_full_unstemmed Early and Late Postnatal Myocardial and Vascular Changes in a Protein Restriction Rat Model of Intrauterine Growth Restriction
title_short Early and Late Postnatal Myocardial and Vascular Changes in a Protein Restriction Rat Model of Intrauterine Growth Restriction
title_sort early and late postnatal myocardial and vascular changes in a protein restriction rat model of intrauterine growth restriction
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3105022/
https://www.ncbi.nlm.nih.gov/pubmed/21655297
http://dx.doi.org/10.1371/journal.pone.0020369
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