Cargando…
Association of Short Tandem Repeat Polymorphism in the Promoter of Prostate Cancer Antigen 3 Gene with the Risk of Prostate Cancer
BACKGROUND: PCA3 (prostate cancer antigen 3) gene is one of the most prostate cancer-specific genes at present. Consequently, the prostate-specific expression and the sharp up-regulation of PCA3 mRNA in prostate cancer suggest a unique transcriptional regulation, which possibly can be attributed to...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3105025/ https://www.ncbi.nlm.nih.gov/pubmed/21655300 http://dx.doi.org/10.1371/journal.pone.0020378 |
_version_ | 1782204670614175744 |
---|---|
author | Zhou, Wu Chen, Zhanguo Hu, Wangqiang Shen, Mo Zhang, Xiaoxia Li, Chengdi Wen, Zhiliang Wu, Xiuling Hu, Yuanping Zhang, Xiaohua Duan, Xiuzhi Han, Xiucui Tao, Zhihua |
author_facet | Zhou, Wu Chen, Zhanguo Hu, Wangqiang Shen, Mo Zhang, Xiaoxia Li, Chengdi Wen, Zhiliang Wu, Xiuling Hu, Yuanping Zhang, Xiaohua Duan, Xiuzhi Han, Xiucui Tao, Zhihua |
author_sort | Zhou, Wu |
collection | PubMed |
description | BACKGROUND: PCA3 (prostate cancer antigen 3) gene is one of the most prostate cancer-specific genes at present. Consequently, the prostate-specific expression and the sharp up-regulation of PCA3 mRNA in prostate cancer suggest a unique transcriptional regulation, which possibly can be attributed to promoter polymorphism. In our study, we evaluated whether there is polymorphism in PCA3 promoter region and also assess the association of the polymorphism with prostate cancer. METHODOLOGY/PRINCIPAL FINDINGS: We designed a specific primer set to screen the promoter of PCA3 gene by polymerase chain reaction (PCR)-based cloning and sequencing with the DNA extracted from peripheral blood samples of prostate cancer (PCa) cases (n = 186) and healthy control cases (n = 135). Genotype-specific risks were estimated as odds ratios (ORs) with associated 95% confidence intervals (CIs) by chi-square test. Possible deviation of the genotype frequencies from controls and PCa cases expected under Hardy-Weinberg equilibrium was assessed by the chi-square test. Short tandem repeat polymorphism of TAAA was found in the promoter region of PCA3 gene, five polymorphisms and eight genotypes were identified. The eight genotypes were divided into three groups: ≤10TAAA, 11TAAA, ≥12TAAA. The group 11TAAA and ≥12TAAA were associated with higher relative risk for prostate cancer than group ≤10TAAA (OR = 1.76, 95%CI = 1.07–2.89[for group 11TAAA]; OR = 5.28, 95%CI = 1.76–15.89[for group ≥12TAAA]). CONCLUSIONS/SIGNIFICANCE: The presence of the (TAAA)n short tandem repeat polymorphisms in the PCA3 promoter region may be a risk factor for prostate cancer in the Chinese population. |
format | Text |
id | pubmed-3105025 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-31050252011-06-08 Association of Short Tandem Repeat Polymorphism in the Promoter of Prostate Cancer Antigen 3 Gene with the Risk of Prostate Cancer Zhou, Wu Chen, Zhanguo Hu, Wangqiang Shen, Mo Zhang, Xiaoxia Li, Chengdi Wen, Zhiliang Wu, Xiuling Hu, Yuanping Zhang, Xiaohua Duan, Xiuzhi Han, Xiucui Tao, Zhihua PLoS One Research Article BACKGROUND: PCA3 (prostate cancer antigen 3) gene is one of the most prostate cancer-specific genes at present. Consequently, the prostate-specific expression and the sharp up-regulation of PCA3 mRNA in prostate cancer suggest a unique transcriptional regulation, which possibly can be attributed to promoter polymorphism. In our study, we evaluated whether there is polymorphism in PCA3 promoter region and also assess the association of the polymorphism with prostate cancer. METHODOLOGY/PRINCIPAL FINDINGS: We designed a specific primer set to screen the promoter of PCA3 gene by polymerase chain reaction (PCR)-based cloning and sequencing with the DNA extracted from peripheral blood samples of prostate cancer (PCa) cases (n = 186) and healthy control cases (n = 135). Genotype-specific risks were estimated as odds ratios (ORs) with associated 95% confidence intervals (CIs) by chi-square test. Possible deviation of the genotype frequencies from controls and PCa cases expected under Hardy-Weinberg equilibrium was assessed by the chi-square test. Short tandem repeat polymorphism of TAAA was found in the promoter region of PCA3 gene, five polymorphisms and eight genotypes were identified. The eight genotypes were divided into three groups: ≤10TAAA, 11TAAA, ≥12TAAA. The group 11TAAA and ≥12TAAA were associated with higher relative risk for prostate cancer than group ≤10TAAA (OR = 1.76, 95%CI = 1.07–2.89[for group 11TAAA]; OR = 5.28, 95%CI = 1.76–15.89[for group ≥12TAAA]). CONCLUSIONS/SIGNIFICANCE: The presence of the (TAAA)n short tandem repeat polymorphisms in the PCA3 promoter region may be a risk factor for prostate cancer in the Chinese population. Public Library of Science 2011-05-31 /pmc/articles/PMC3105025/ /pubmed/21655300 http://dx.doi.org/10.1371/journal.pone.0020378 Text en Zhou et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Zhou, Wu Chen, Zhanguo Hu, Wangqiang Shen, Mo Zhang, Xiaoxia Li, Chengdi Wen, Zhiliang Wu, Xiuling Hu, Yuanping Zhang, Xiaohua Duan, Xiuzhi Han, Xiucui Tao, Zhihua Association of Short Tandem Repeat Polymorphism in the Promoter of Prostate Cancer Antigen 3 Gene with the Risk of Prostate Cancer |
title | Association of Short Tandem Repeat Polymorphism in the Promoter of Prostate Cancer Antigen 3 Gene with the Risk of Prostate Cancer |
title_full | Association of Short Tandem Repeat Polymorphism in the Promoter of Prostate Cancer Antigen 3 Gene with the Risk of Prostate Cancer |
title_fullStr | Association of Short Tandem Repeat Polymorphism in the Promoter of Prostate Cancer Antigen 3 Gene with the Risk of Prostate Cancer |
title_full_unstemmed | Association of Short Tandem Repeat Polymorphism in the Promoter of Prostate Cancer Antigen 3 Gene with the Risk of Prostate Cancer |
title_short | Association of Short Tandem Repeat Polymorphism in the Promoter of Prostate Cancer Antigen 3 Gene with the Risk of Prostate Cancer |
title_sort | association of short tandem repeat polymorphism in the promoter of prostate cancer antigen 3 gene with the risk of prostate cancer |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3105025/ https://www.ncbi.nlm.nih.gov/pubmed/21655300 http://dx.doi.org/10.1371/journal.pone.0020378 |
work_keys_str_mv | AT zhouwu associationofshorttandemrepeatpolymorphisminthepromoterofprostatecancerantigen3genewiththeriskofprostatecancer AT chenzhanguo associationofshorttandemrepeatpolymorphisminthepromoterofprostatecancerantigen3genewiththeriskofprostatecancer AT huwangqiang associationofshorttandemrepeatpolymorphisminthepromoterofprostatecancerantigen3genewiththeriskofprostatecancer AT shenmo associationofshorttandemrepeatpolymorphisminthepromoterofprostatecancerantigen3genewiththeriskofprostatecancer AT zhangxiaoxia associationofshorttandemrepeatpolymorphisminthepromoterofprostatecancerantigen3genewiththeriskofprostatecancer AT lichengdi associationofshorttandemrepeatpolymorphisminthepromoterofprostatecancerantigen3genewiththeriskofprostatecancer AT wenzhiliang associationofshorttandemrepeatpolymorphisminthepromoterofprostatecancerantigen3genewiththeriskofprostatecancer AT wuxiuling associationofshorttandemrepeatpolymorphisminthepromoterofprostatecancerantigen3genewiththeriskofprostatecancer AT huyuanping associationofshorttandemrepeatpolymorphisminthepromoterofprostatecancerantigen3genewiththeriskofprostatecancer AT zhangxiaohua associationofshorttandemrepeatpolymorphisminthepromoterofprostatecancerantigen3genewiththeriskofprostatecancer AT duanxiuzhi associationofshorttandemrepeatpolymorphisminthepromoterofprostatecancerantigen3genewiththeriskofprostatecancer AT hanxiucui associationofshorttandemrepeatpolymorphisminthepromoterofprostatecancerantigen3genewiththeriskofprostatecancer AT taozhihua associationofshorttandemrepeatpolymorphisminthepromoterofprostatecancerantigen3genewiththeriskofprostatecancer |