Cargando…

Association of Short Tandem Repeat Polymorphism in the Promoter of Prostate Cancer Antigen 3 Gene with the Risk of Prostate Cancer

BACKGROUND: PCA3 (prostate cancer antigen 3) gene is one of the most prostate cancer-specific genes at present. Consequently, the prostate-specific expression and the sharp up-regulation of PCA3 mRNA in prostate cancer suggest a unique transcriptional regulation, which possibly can be attributed to...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhou, Wu, Chen, Zhanguo, Hu, Wangqiang, Shen, Mo, Zhang, Xiaoxia, Li, Chengdi, Wen, Zhiliang, Wu, Xiuling, Hu, Yuanping, Zhang, Xiaohua, Duan, Xiuzhi, Han, Xiucui, Tao, Zhihua
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3105025/
https://www.ncbi.nlm.nih.gov/pubmed/21655300
http://dx.doi.org/10.1371/journal.pone.0020378
_version_ 1782204670614175744
author Zhou, Wu
Chen, Zhanguo
Hu, Wangqiang
Shen, Mo
Zhang, Xiaoxia
Li, Chengdi
Wen, Zhiliang
Wu, Xiuling
Hu, Yuanping
Zhang, Xiaohua
Duan, Xiuzhi
Han, Xiucui
Tao, Zhihua
author_facet Zhou, Wu
Chen, Zhanguo
Hu, Wangqiang
Shen, Mo
Zhang, Xiaoxia
Li, Chengdi
Wen, Zhiliang
Wu, Xiuling
Hu, Yuanping
Zhang, Xiaohua
Duan, Xiuzhi
Han, Xiucui
Tao, Zhihua
author_sort Zhou, Wu
collection PubMed
description BACKGROUND: PCA3 (prostate cancer antigen 3) gene is one of the most prostate cancer-specific genes at present. Consequently, the prostate-specific expression and the sharp up-regulation of PCA3 mRNA in prostate cancer suggest a unique transcriptional regulation, which possibly can be attributed to promoter polymorphism. In our study, we evaluated whether there is polymorphism in PCA3 promoter region and also assess the association of the polymorphism with prostate cancer. METHODOLOGY/PRINCIPAL FINDINGS: We designed a specific primer set to screen the promoter of PCA3 gene by polymerase chain reaction (PCR)-based cloning and sequencing with the DNA extracted from peripheral blood samples of prostate cancer (PCa) cases (n = 186) and healthy control cases (n = 135). Genotype-specific risks were estimated as odds ratios (ORs) with associated 95% confidence intervals (CIs) by chi-square test. Possible deviation of the genotype frequencies from controls and PCa cases expected under Hardy-Weinberg equilibrium was assessed by the chi-square test. Short tandem repeat polymorphism of TAAA was found in the promoter region of PCA3 gene, five polymorphisms and eight genotypes were identified. The eight genotypes were divided into three groups: ≤10TAAA, 11TAAA, ≥12TAAA. The group 11TAAA and ≥12TAAA were associated with higher relative risk for prostate cancer than group ≤10TAAA (OR = 1.76, 95%CI = 1.07–2.89[for group 11TAAA]; OR = 5.28, 95%CI = 1.76–15.89[for group ≥12TAAA]). CONCLUSIONS/SIGNIFICANCE: The presence of the (TAAA)n short tandem repeat polymorphisms in the PCA3 promoter region may be a risk factor for prostate cancer in the Chinese population.
format Text
id pubmed-3105025
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-31050252011-06-08 Association of Short Tandem Repeat Polymorphism in the Promoter of Prostate Cancer Antigen 3 Gene with the Risk of Prostate Cancer Zhou, Wu Chen, Zhanguo Hu, Wangqiang Shen, Mo Zhang, Xiaoxia Li, Chengdi Wen, Zhiliang Wu, Xiuling Hu, Yuanping Zhang, Xiaohua Duan, Xiuzhi Han, Xiucui Tao, Zhihua PLoS One Research Article BACKGROUND: PCA3 (prostate cancer antigen 3) gene is one of the most prostate cancer-specific genes at present. Consequently, the prostate-specific expression and the sharp up-regulation of PCA3 mRNA in prostate cancer suggest a unique transcriptional regulation, which possibly can be attributed to promoter polymorphism. In our study, we evaluated whether there is polymorphism in PCA3 promoter region and also assess the association of the polymorphism with prostate cancer. METHODOLOGY/PRINCIPAL FINDINGS: We designed a specific primer set to screen the promoter of PCA3 gene by polymerase chain reaction (PCR)-based cloning and sequencing with the DNA extracted from peripheral blood samples of prostate cancer (PCa) cases (n = 186) and healthy control cases (n = 135). Genotype-specific risks were estimated as odds ratios (ORs) with associated 95% confidence intervals (CIs) by chi-square test. Possible deviation of the genotype frequencies from controls and PCa cases expected under Hardy-Weinberg equilibrium was assessed by the chi-square test. Short tandem repeat polymorphism of TAAA was found in the promoter region of PCA3 gene, five polymorphisms and eight genotypes were identified. The eight genotypes were divided into three groups: ≤10TAAA, 11TAAA, ≥12TAAA. The group 11TAAA and ≥12TAAA were associated with higher relative risk for prostate cancer than group ≤10TAAA (OR = 1.76, 95%CI = 1.07–2.89[for group 11TAAA]; OR = 5.28, 95%CI = 1.76–15.89[for group ≥12TAAA]). CONCLUSIONS/SIGNIFICANCE: The presence of the (TAAA)n short tandem repeat polymorphisms in the PCA3 promoter region may be a risk factor for prostate cancer in the Chinese population. Public Library of Science 2011-05-31 /pmc/articles/PMC3105025/ /pubmed/21655300 http://dx.doi.org/10.1371/journal.pone.0020378 Text en Zhou et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zhou, Wu
Chen, Zhanguo
Hu, Wangqiang
Shen, Mo
Zhang, Xiaoxia
Li, Chengdi
Wen, Zhiliang
Wu, Xiuling
Hu, Yuanping
Zhang, Xiaohua
Duan, Xiuzhi
Han, Xiucui
Tao, Zhihua
Association of Short Tandem Repeat Polymorphism in the Promoter of Prostate Cancer Antigen 3 Gene with the Risk of Prostate Cancer
title Association of Short Tandem Repeat Polymorphism in the Promoter of Prostate Cancer Antigen 3 Gene with the Risk of Prostate Cancer
title_full Association of Short Tandem Repeat Polymorphism in the Promoter of Prostate Cancer Antigen 3 Gene with the Risk of Prostate Cancer
title_fullStr Association of Short Tandem Repeat Polymorphism in the Promoter of Prostate Cancer Antigen 3 Gene with the Risk of Prostate Cancer
title_full_unstemmed Association of Short Tandem Repeat Polymorphism in the Promoter of Prostate Cancer Antigen 3 Gene with the Risk of Prostate Cancer
title_short Association of Short Tandem Repeat Polymorphism in the Promoter of Prostate Cancer Antigen 3 Gene with the Risk of Prostate Cancer
title_sort association of short tandem repeat polymorphism in the promoter of prostate cancer antigen 3 gene with the risk of prostate cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3105025/
https://www.ncbi.nlm.nih.gov/pubmed/21655300
http://dx.doi.org/10.1371/journal.pone.0020378
work_keys_str_mv AT zhouwu associationofshorttandemrepeatpolymorphisminthepromoterofprostatecancerantigen3genewiththeriskofprostatecancer
AT chenzhanguo associationofshorttandemrepeatpolymorphisminthepromoterofprostatecancerantigen3genewiththeriskofprostatecancer
AT huwangqiang associationofshorttandemrepeatpolymorphisminthepromoterofprostatecancerantigen3genewiththeriskofprostatecancer
AT shenmo associationofshorttandemrepeatpolymorphisminthepromoterofprostatecancerantigen3genewiththeriskofprostatecancer
AT zhangxiaoxia associationofshorttandemrepeatpolymorphisminthepromoterofprostatecancerantigen3genewiththeriskofprostatecancer
AT lichengdi associationofshorttandemrepeatpolymorphisminthepromoterofprostatecancerantigen3genewiththeriskofprostatecancer
AT wenzhiliang associationofshorttandemrepeatpolymorphisminthepromoterofprostatecancerantigen3genewiththeriskofprostatecancer
AT wuxiuling associationofshorttandemrepeatpolymorphisminthepromoterofprostatecancerantigen3genewiththeriskofprostatecancer
AT huyuanping associationofshorttandemrepeatpolymorphisminthepromoterofprostatecancerantigen3genewiththeriskofprostatecancer
AT zhangxiaohua associationofshorttandemrepeatpolymorphisminthepromoterofprostatecancerantigen3genewiththeriskofprostatecancer
AT duanxiuzhi associationofshorttandemrepeatpolymorphisminthepromoterofprostatecancerantigen3genewiththeriskofprostatecancer
AT hanxiucui associationofshorttandemrepeatpolymorphisminthepromoterofprostatecancerantigen3genewiththeriskofprostatecancer
AT taozhihua associationofshorttandemrepeatpolymorphisminthepromoterofprostatecancerantigen3genewiththeriskofprostatecancer