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Identification of Novel Schizophrenia Loci by Homozygosity Mapping Using DNA Microarray Analysis

The recent development of high-resolution DNA microarrays, in which hundreds of thousands of single nucleotide polymorphisms (SNPs) are genotyped, enables the rapid identification of susceptibility genes for complex diseases. Clusters of these SNPs may show runs of homozygosity (ROHs) that can be an...

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Autores principales: Kurotaki, Naohiro, Tasaki, Shinya, Mishima, Hiroyuki, Ono, Shinji, Imamura, Akira, Kikuchi, Taeko, Nishida, Nao, Tokunaga, Katsushi, Yoshiura, Koh-ichiro, Ozawa, Hiroki
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3105082/
https://www.ncbi.nlm.nih.gov/pubmed/21655227
http://dx.doi.org/10.1371/journal.pone.0020589
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author Kurotaki, Naohiro
Tasaki, Shinya
Mishima, Hiroyuki
Ono, Shinji
Imamura, Akira
Kikuchi, Taeko
Nishida, Nao
Tokunaga, Katsushi
Yoshiura, Koh-ichiro
Ozawa, Hiroki
author_facet Kurotaki, Naohiro
Tasaki, Shinya
Mishima, Hiroyuki
Ono, Shinji
Imamura, Akira
Kikuchi, Taeko
Nishida, Nao
Tokunaga, Katsushi
Yoshiura, Koh-ichiro
Ozawa, Hiroki
author_sort Kurotaki, Naohiro
collection PubMed
description The recent development of high-resolution DNA microarrays, in which hundreds of thousands of single nucleotide polymorphisms (SNPs) are genotyped, enables the rapid identification of susceptibility genes for complex diseases. Clusters of these SNPs may show runs of homozygosity (ROHs) that can be analyzed for association with disease. An analysis of patients whose parents were first cousins enables the search for autozygous segments in their offspring. Here, using the Affymetrix® Genome-Wide Human SNP Array 5.0 to determine ROHs, we genotyped 9 individuals with schizophrenia (SCZ) whose parents were first cousins. We identified overlapping ROHs on chromosomes 1, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 16, 17, 19, 20, and 21 in at least 3 individuals. Only the locus on chromosome 5 has been reported previously. The ROHs on chromosome 5q23.3–q31.1 include the candidate genes histidine triad nucleotide binding protein 1 (HINT1) and acyl-CoA synthetase long-chain family member 6 (ACSL6). Other overlapping ROHs may contain novel rare recessive variants that affect SCZ specifically in our samples, given the highly heterozygous nature of SCZ. Analysis of patients whose parents are first cousins may provide new insights for the genetic analysis of psychiatric diseases.
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spelling pubmed-31050822011-06-08 Identification of Novel Schizophrenia Loci by Homozygosity Mapping Using DNA Microarray Analysis Kurotaki, Naohiro Tasaki, Shinya Mishima, Hiroyuki Ono, Shinji Imamura, Akira Kikuchi, Taeko Nishida, Nao Tokunaga, Katsushi Yoshiura, Koh-ichiro Ozawa, Hiroki PLoS One Research Article The recent development of high-resolution DNA microarrays, in which hundreds of thousands of single nucleotide polymorphisms (SNPs) are genotyped, enables the rapid identification of susceptibility genes for complex diseases. Clusters of these SNPs may show runs of homozygosity (ROHs) that can be analyzed for association with disease. An analysis of patients whose parents were first cousins enables the search for autozygous segments in their offspring. Here, using the Affymetrix® Genome-Wide Human SNP Array 5.0 to determine ROHs, we genotyped 9 individuals with schizophrenia (SCZ) whose parents were first cousins. We identified overlapping ROHs on chromosomes 1, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 16, 17, 19, 20, and 21 in at least 3 individuals. Only the locus on chromosome 5 has been reported previously. The ROHs on chromosome 5q23.3–q31.1 include the candidate genes histidine triad nucleotide binding protein 1 (HINT1) and acyl-CoA synthetase long-chain family member 6 (ACSL6). Other overlapping ROHs may contain novel rare recessive variants that affect SCZ specifically in our samples, given the highly heterozygous nature of SCZ. Analysis of patients whose parents are first cousins may provide new insights for the genetic analysis of psychiatric diseases. Public Library of Science 2011-05-31 /pmc/articles/PMC3105082/ /pubmed/21655227 http://dx.doi.org/10.1371/journal.pone.0020589 Text en Kurotaki et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kurotaki, Naohiro
Tasaki, Shinya
Mishima, Hiroyuki
Ono, Shinji
Imamura, Akira
Kikuchi, Taeko
Nishida, Nao
Tokunaga, Katsushi
Yoshiura, Koh-ichiro
Ozawa, Hiroki
Identification of Novel Schizophrenia Loci by Homozygosity Mapping Using DNA Microarray Analysis
title Identification of Novel Schizophrenia Loci by Homozygosity Mapping Using DNA Microarray Analysis
title_full Identification of Novel Schizophrenia Loci by Homozygosity Mapping Using DNA Microarray Analysis
title_fullStr Identification of Novel Schizophrenia Loci by Homozygosity Mapping Using DNA Microarray Analysis
title_full_unstemmed Identification of Novel Schizophrenia Loci by Homozygosity Mapping Using DNA Microarray Analysis
title_short Identification of Novel Schizophrenia Loci by Homozygosity Mapping Using DNA Microarray Analysis
title_sort identification of novel schizophrenia loci by homozygosity mapping using dna microarray analysis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3105082/
https://www.ncbi.nlm.nih.gov/pubmed/21655227
http://dx.doi.org/10.1371/journal.pone.0020589
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