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Type I Interferon Drives Dendritic Cell Apoptosis via Multiple BH3-Only Proteins following Activation by PolyIC In Vivo

BACKGROUND: DC are activated by pathogen-associated molecular patterns (PAMPs), and this is pivotal for the induction of adaptive immune responses. Thereafter, the clearance of activated DC is crucial to prevent immune pathology. While PAMPs are of major interest for vaccine science due to their adj...

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Autores principales: Fuertes Marraco, Silvia A., Scott, Clare L., Bouillet, Philippe, Ives, Annette, Masina, Slavica, Vremec, David, Jansen, Elisa S., O'Reilly, Lorraine A., Schneider, Pascal, Fasel, Nicolas, Shortman, Ken, Strasser, Andreas, Acha-Orbea, Hans
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3107228/
https://www.ncbi.nlm.nih.gov/pubmed/21674051
http://dx.doi.org/10.1371/journal.pone.0020189
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author Fuertes Marraco, Silvia A.
Scott, Clare L.
Bouillet, Philippe
Ives, Annette
Masina, Slavica
Vremec, David
Jansen, Elisa S.
O'Reilly, Lorraine A.
Schneider, Pascal
Fasel, Nicolas
Shortman, Ken
Strasser, Andreas
Acha-Orbea, Hans
author_facet Fuertes Marraco, Silvia A.
Scott, Clare L.
Bouillet, Philippe
Ives, Annette
Masina, Slavica
Vremec, David
Jansen, Elisa S.
O'Reilly, Lorraine A.
Schneider, Pascal
Fasel, Nicolas
Shortman, Ken
Strasser, Andreas
Acha-Orbea, Hans
author_sort Fuertes Marraco, Silvia A.
collection PubMed
description BACKGROUND: DC are activated by pathogen-associated molecular patterns (PAMPs), and this is pivotal for the induction of adaptive immune responses. Thereafter, the clearance of activated DC is crucial to prevent immune pathology. While PAMPs are of major interest for vaccine science due to their adjuvant potential, it is unclear whether and how PAMPs may affect DC viability. We aimed to elucidate the possible apoptotic mechanisms that control activated DC lifespan in response to PAMPs, particularly in vivo. METHODOLOGY/PRINCIPAL FINDINGS: We report that polyinosinic:polycytidylic acid (PolyIC, synthetic analogue of dsRNA) induces dramatic apoptosis of mouse splenic conventional DC (cDC) in vivo, predominantly affecting the CD8α subset, as shown by flow cytometry-based analysis of splenic DC subsets. Importantly, while Bim deficiency conferred only minor protection, cDC depletion was prevented in mice lacking Bim plus one of three other BH3-only proteins, either Puma, Noxa or Bid. Furthermore, we show that Type I Interferon (IFN) is necessary and sufficient for DC death both in vitro and in vivo, and that TLR3 and MAVS co-operate in IFNß production in vivo to induce DC death in response to PolyIC. CONCLUSIONS/SIGNIFICANCE: These results demonstrate for the first time in vivo that apoptosis restricts DC lifespan following activation by PolyIC, particularly affecting the CD8α cDC subset. Such DC apoptosis is mediated by the overlapping action of pro-apoptotic BH3-only proteins, including but not solely involving Bim, and is driven by Type I IFN. While Type I IFNs are important anti-viral factors, CD8α cDC are major cross-presenting cells and critical inducers of CTL. We discuss such paradoxical finding on DC death with PolyIC/Type I IFN. These results could contribute to understand immunosuppression associated with chronic infection, and to the optimization of DC-based therapies and the clinical use of PAMPs and Type I IFNs.
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spelling pubmed-31072282011-06-13 Type I Interferon Drives Dendritic Cell Apoptosis via Multiple BH3-Only Proteins following Activation by PolyIC In Vivo Fuertes Marraco, Silvia A. Scott, Clare L. Bouillet, Philippe Ives, Annette Masina, Slavica Vremec, David Jansen, Elisa S. O'Reilly, Lorraine A. Schneider, Pascal Fasel, Nicolas Shortman, Ken Strasser, Andreas Acha-Orbea, Hans PLoS One Research Article BACKGROUND: DC are activated by pathogen-associated molecular patterns (PAMPs), and this is pivotal for the induction of adaptive immune responses. Thereafter, the clearance of activated DC is crucial to prevent immune pathology. While PAMPs are of major interest for vaccine science due to their adjuvant potential, it is unclear whether and how PAMPs may affect DC viability. We aimed to elucidate the possible apoptotic mechanisms that control activated DC lifespan in response to PAMPs, particularly in vivo. METHODOLOGY/PRINCIPAL FINDINGS: We report that polyinosinic:polycytidylic acid (PolyIC, synthetic analogue of dsRNA) induces dramatic apoptosis of mouse splenic conventional DC (cDC) in vivo, predominantly affecting the CD8α subset, as shown by flow cytometry-based analysis of splenic DC subsets. Importantly, while Bim deficiency conferred only minor protection, cDC depletion was prevented in mice lacking Bim plus one of three other BH3-only proteins, either Puma, Noxa or Bid. Furthermore, we show that Type I Interferon (IFN) is necessary and sufficient for DC death both in vitro and in vivo, and that TLR3 and MAVS co-operate in IFNß production in vivo to induce DC death in response to PolyIC. CONCLUSIONS/SIGNIFICANCE: These results demonstrate for the first time in vivo that apoptosis restricts DC lifespan following activation by PolyIC, particularly affecting the CD8α cDC subset. Such DC apoptosis is mediated by the overlapping action of pro-apoptotic BH3-only proteins, including but not solely involving Bim, and is driven by Type I IFN. While Type I IFNs are important anti-viral factors, CD8α cDC are major cross-presenting cells and critical inducers of CTL. We discuss such paradoxical finding on DC death with PolyIC/Type I IFN. These results could contribute to understand immunosuppression associated with chronic infection, and to the optimization of DC-based therapies and the clinical use of PAMPs and Type I IFNs. Public Library of Science 2011-06-02 /pmc/articles/PMC3107228/ /pubmed/21674051 http://dx.doi.org/10.1371/journal.pone.0020189 Text en Fuertes Marraco et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Fuertes Marraco, Silvia A.
Scott, Clare L.
Bouillet, Philippe
Ives, Annette
Masina, Slavica
Vremec, David
Jansen, Elisa S.
O'Reilly, Lorraine A.
Schneider, Pascal
Fasel, Nicolas
Shortman, Ken
Strasser, Andreas
Acha-Orbea, Hans
Type I Interferon Drives Dendritic Cell Apoptosis via Multiple BH3-Only Proteins following Activation by PolyIC In Vivo
title Type I Interferon Drives Dendritic Cell Apoptosis via Multiple BH3-Only Proteins following Activation by PolyIC In Vivo
title_full Type I Interferon Drives Dendritic Cell Apoptosis via Multiple BH3-Only Proteins following Activation by PolyIC In Vivo
title_fullStr Type I Interferon Drives Dendritic Cell Apoptosis via Multiple BH3-Only Proteins following Activation by PolyIC In Vivo
title_full_unstemmed Type I Interferon Drives Dendritic Cell Apoptosis via Multiple BH3-Only Proteins following Activation by PolyIC In Vivo
title_short Type I Interferon Drives Dendritic Cell Apoptosis via Multiple BH3-Only Proteins following Activation by PolyIC In Vivo
title_sort type i interferon drives dendritic cell apoptosis via multiple bh3-only proteins following activation by polyic in vivo
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3107228/
https://www.ncbi.nlm.nih.gov/pubmed/21674051
http://dx.doi.org/10.1371/journal.pone.0020189
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