Cargando…

Dynamic distribution and tissue tropism of classical swine fever virus in experimentally infected pigs

BACKGROUND: Classical swine fever (CSF), caused by the Classical swine fever virus (CSFV), is an Office International des Epizooties (OIE) notifiable disease. However, we are far from fully understand the distribution, tissue tropism, pathogenesis, replication and excretion of CSFV in pigs. In this...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Jun, Fan, Xue-Zheng, Wang, Qin, Xu, Lu, Zhao, Qi-Zu, Huang, Wei, Zhou, Yuan-Cheng, Tang, Bo, Chen, Lei, Zou, Xing-Qi, Sha, Sha, Zhu, Yuan-Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3107811/
https://www.ncbi.nlm.nih.gov/pubmed/21535885
http://dx.doi.org/10.1186/1743-422X-8-201
_version_ 1782205252229922816
author Liu, Jun
Fan, Xue-Zheng
Wang, Qin
Xu, Lu
Zhao, Qi-Zu
Huang, Wei
Zhou, Yuan-Cheng
Tang, Bo
Chen, Lei
Zou, Xing-Qi
Sha, Sha
Zhu, Yuan-Yuan
author_facet Liu, Jun
Fan, Xue-Zheng
Wang, Qin
Xu, Lu
Zhao, Qi-Zu
Huang, Wei
Zhou, Yuan-Cheng
Tang, Bo
Chen, Lei
Zou, Xing-Qi
Sha, Sha
Zhu, Yuan-Yuan
author_sort Liu, Jun
collection PubMed
description BACKGROUND: Classical swine fever (CSF), caused by the Classical swine fever virus (CSFV), is an Office International des Epizooties (OIE) notifiable disease. However, we are far from fully understand the distribution, tissue tropism, pathogenesis, replication and excretion of CSFV in pigs. In this report, we investigated the dynamic distribution and tissue tropism of the virus in internal organs of the experimentally infected pigs using real-time RT-PCR and immunohistochemistry (IHC). RESULTS: A relative quantification real-time PCR was established and used to detect the virus load in internal organs of the experimentally infected pigs. The study revealed that the virus was detected in all 21 of the internal organs and blood collected from pigs at day 1 to day 8 post infections, and had an increasing virus load from day 1 to day 8 post infections. However, there was irregular distribution virus load in most internal organs over the first 2 days post infection. Blood, lymphoid tissue, pancreas and ileum usually contain the highest viral loads, while heart, duodenum and brain show relatively low viral loads. CONCLUSIONS: All the data suggest that CSFV had an increasing virus load from day 1 to day 8 post infections in experimentally infected pigs detected by real-time RT-PCR, which was in consistent with the result of the IHC staining. The data also show that CSFV was likely to reproduce in blood, lymphoid tissue, pancreas and the ileum, while unlikely to replicate in the heart, duodenum and brain. The results provide a foundation for further clarification of the pathogenic mechanism of CSFV in internal organs, and indicate that blood, lymphoid tissue, pancreas and ileum may be preferred sites of acute infection.
format Online
Article
Text
id pubmed-3107811
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-31078112011-06-04 Dynamic distribution and tissue tropism of classical swine fever virus in experimentally infected pigs Liu, Jun Fan, Xue-Zheng Wang, Qin Xu, Lu Zhao, Qi-Zu Huang, Wei Zhou, Yuan-Cheng Tang, Bo Chen, Lei Zou, Xing-Qi Sha, Sha Zhu, Yuan-Yuan Virol J Research BACKGROUND: Classical swine fever (CSF), caused by the Classical swine fever virus (CSFV), is an Office International des Epizooties (OIE) notifiable disease. However, we are far from fully understand the distribution, tissue tropism, pathogenesis, replication and excretion of CSFV in pigs. In this report, we investigated the dynamic distribution and tissue tropism of the virus in internal organs of the experimentally infected pigs using real-time RT-PCR and immunohistochemistry (IHC). RESULTS: A relative quantification real-time PCR was established and used to detect the virus load in internal organs of the experimentally infected pigs. The study revealed that the virus was detected in all 21 of the internal organs and blood collected from pigs at day 1 to day 8 post infections, and had an increasing virus load from day 1 to day 8 post infections. However, there was irregular distribution virus load in most internal organs over the first 2 days post infection. Blood, lymphoid tissue, pancreas and ileum usually contain the highest viral loads, while heart, duodenum and brain show relatively low viral loads. CONCLUSIONS: All the data suggest that CSFV had an increasing virus load from day 1 to day 8 post infections in experimentally infected pigs detected by real-time RT-PCR, which was in consistent with the result of the IHC staining. The data also show that CSFV was likely to reproduce in blood, lymphoid tissue, pancreas and the ileum, while unlikely to replicate in the heart, duodenum and brain. The results provide a foundation for further clarification of the pathogenic mechanism of CSFV in internal organs, and indicate that blood, lymphoid tissue, pancreas and ileum may be preferred sites of acute infection. BioMed Central 2011-05-02 /pmc/articles/PMC3107811/ /pubmed/21535885 http://dx.doi.org/10.1186/1743-422X-8-201 Text en Copyright ©2011 Liu et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Liu, Jun
Fan, Xue-Zheng
Wang, Qin
Xu, Lu
Zhao, Qi-Zu
Huang, Wei
Zhou, Yuan-Cheng
Tang, Bo
Chen, Lei
Zou, Xing-Qi
Sha, Sha
Zhu, Yuan-Yuan
Dynamic distribution and tissue tropism of classical swine fever virus in experimentally infected pigs
title Dynamic distribution and tissue tropism of classical swine fever virus in experimentally infected pigs
title_full Dynamic distribution and tissue tropism of classical swine fever virus in experimentally infected pigs
title_fullStr Dynamic distribution and tissue tropism of classical swine fever virus in experimentally infected pigs
title_full_unstemmed Dynamic distribution and tissue tropism of classical swine fever virus in experimentally infected pigs
title_short Dynamic distribution and tissue tropism of classical swine fever virus in experimentally infected pigs
title_sort dynamic distribution and tissue tropism of classical swine fever virus in experimentally infected pigs
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3107811/
https://www.ncbi.nlm.nih.gov/pubmed/21535885
http://dx.doi.org/10.1186/1743-422X-8-201
work_keys_str_mv AT liujun dynamicdistributionandtissuetropismofclassicalswinefevervirusinexperimentallyinfectedpigs
AT fanxuezheng dynamicdistributionandtissuetropismofclassicalswinefevervirusinexperimentallyinfectedpigs
AT wangqin dynamicdistributionandtissuetropismofclassicalswinefevervirusinexperimentallyinfectedpigs
AT xulu dynamicdistributionandtissuetropismofclassicalswinefevervirusinexperimentallyinfectedpigs
AT zhaoqizu dynamicdistributionandtissuetropismofclassicalswinefevervirusinexperimentallyinfectedpigs
AT huangwei dynamicdistributionandtissuetropismofclassicalswinefevervirusinexperimentallyinfectedpigs
AT zhouyuancheng dynamicdistributionandtissuetropismofclassicalswinefevervirusinexperimentallyinfectedpigs
AT tangbo dynamicdistributionandtissuetropismofclassicalswinefevervirusinexperimentallyinfectedpigs
AT chenlei dynamicdistributionandtissuetropismofclassicalswinefevervirusinexperimentallyinfectedpigs
AT zouxingqi dynamicdistributionandtissuetropismofclassicalswinefevervirusinexperimentallyinfectedpigs
AT shasha dynamicdistributionandtissuetropismofclassicalswinefevervirusinexperimentallyinfectedpigs
AT zhuyuanyuan dynamicdistributionandtissuetropismofclassicalswinefevervirusinexperimentallyinfectedpigs