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Gene expression pattern in swine neutrophils after lipopolysaccharide exposure: a time course comparison

BACKGROUND: Experimental exposure of swine neutrophils to bacterial lipopolysaccharide (LPS) represents a model to study the innate immune response during bacterial infection. Neutrophils can effectively limit the infection by secreting lipid mediators, antimicrobial molecules and a combination of r...

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Autores principales: Sanz-Santos, Gema, Jiménez-Marín, Ángeles, Bautista, Rocío, Fernández, Noé, Claros, Gonzalo M, Garrido, Juan J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3108205/
https://www.ncbi.nlm.nih.gov/pubmed/21645290
http://dx.doi.org/10.1186/1753-6561-5-S4-S11
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author Sanz-Santos, Gema
Jiménez-Marín, Ángeles
Bautista, Rocío
Fernández, Noé
Claros, Gonzalo M
Garrido, Juan J
author_facet Sanz-Santos, Gema
Jiménez-Marín, Ángeles
Bautista, Rocío
Fernández, Noé
Claros, Gonzalo M
Garrido, Juan J
author_sort Sanz-Santos, Gema
collection PubMed
description BACKGROUND: Experimental exposure of swine neutrophils to bacterial lipopolysaccharide (LPS) represents a model to study the innate immune response during bacterial infection. Neutrophils can effectively limit the infection by secreting lipid mediators, antimicrobial molecules and a combination of reactive oxygen species (ROS) without new synthesis of proteins. However, it is known that neutrophils can modify the gene expression after LPS exposure. We performed microarray gene expression analysis in order to elucidate the less known transcriptional response of neutrophils during infection. METHODS: Blood samples were collected from four healthy Iberian pigs and neutrophils were isolated and incubated during 6, 9 and 18 hrs in presence or absence of lipopolysaccharide (LPS) from Salmonella enterica serovar Typhimurium. RNA was isolated and hybridized to Affymetrix Porcine GeneChip(®). Microarray data were normalized using Robust Microarray Analysis (RMA) and then, differential expression was obtained by an analysis of variance (ANOVA). RESULTS: ANOVA data analysis showed that the number of differentially expressed genes (DEG) after LPS treatment vary with time. The highest transcriptional response occurred at 9 hr post LPS stimulation with 1494 DEG whereas at 6 and 18 hr showed 125 and 108 DEG, respectively. Three different gene expression tendencies were observed: genes in cluster 1 showed a tendency toward up-regulation; cluster 2 genes showing a tendency for down-regulation at 9 hr; and cluster 3 genes were up-regulated at 9 hr post LPS stimulation. Ingenuity Pathway Analysis revealed a delay of neutrophil apoptosis at 9 hr. Many genes controlling biological functions were altered with time including those controlling metabolism and cell organization, ubiquitination, adhesion, movement or inflammatory response. CONCLUSIONS: LPS stimulation alters the transcriptional pattern in neutrophils and the present results show that the robust transcriptional potential of neutrophils under infection conditions, indicating that active regulation of gene expression plays a major role in the neutrophil-mediated- innate immune response.
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spelling pubmed-31082052011-06-07 Gene expression pattern in swine neutrophils after lipopolysaccharide exposure: a time course comparison Sanz-Santos, Gema Jiménez-Marín, Ángeles Bautista, Rocío Fernández, Noé Claros, Gonzalo M Garrido, Juan J BMC Proc Proceedings BACKGROUND: Experimental exposure of swine neutrophils to bacterial lipopolysaccharide (LPS) represents a model to study the innate immune response during bacterial infection. Neutrophils can effectively limit the infection by secreting lipid mediators, antimicrobial molecules and a combination of reactive oxygen species (ROS) without new synthesis of proteins. However, it is known that neutrophils can modify the gene expression after LPS exposure. We performed microarray gene expression analysis in order to elucidate the less known transcriptional response of neutrophils during infection. METHODS: Blood samples were collected from four healthy Iberian pigs and neutrophils were isolated and incubated during 6, 9 and 18 hrs in presence or absence of lipopolysaccharide (LPS) from Salmonella enterica serovar Typhimurium. RNA was isolated and hybridized to Affymetrix Porcine GeneChip(®). Microarray data were normalized using Robust Microarray Analysis (RMA) and then, differential expression was obtained by an analysis of variance (ANOVA). RESULTS: ANOVA data analysis showed that the number of differentially expressed genes (DEG) after LPS treatment vary with time. The highest transcriptional response occurred at 9 hr post LPS stimulation with 1494 DEG whereas at 6 and 18 hr showed 125 and 108 DEG, respectively. Three different gene expression tendencies were observed: genes in cluster 1 showed a tendency toward up-regulation; cluster 2 genes showing a tendency for down-regulation at 9 hr; and cluster 3 genes were up-regulated at 9 hr post LPS stimulation. Ingenuity Pathway Analysis revealed a delay of neutrophil apoptosis at 9 hr. Many genes controlling biological functions were altered with time including those controlling metabolism and cell organization, ubiquitination, adhesion, movement or inflammatory response. CONCLUSIONS: LPS stimulation alters the transcriptional pattern in neutrophils and the present results show that the robust transcriptional potential of neutrophils under infection conditions, indicating that active regulation of gene expression plays a major role in the neutrophil-mediated- innate immune response. BioMed Central 2011-06-03 /pmc/articles/PMC3108205/ /pubmed/21645290 http://dx.doi.org/10.1186/1753-6561-5-S4-S11 Text en Copyright ©2011 Sanz-Santos et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Proceedings
Sanz-Santos, Gema
Jiménez-Marín, Ángeles
Bautista, Rocío
Fernández, Noé
Claros, Gonzalo M
Garrido, Juan J
Gene expression pattern in swine neutrophils after lipopolysaccharide exposure: a time course comparison
title Gene expression pattern in swine neutrophils after lipopolysaccharide exposure: a time course comparison
title_full Gene expression pattern in swine neutrophils after lipopolysaccharide exposure: a time course comparison
title_fullStr Gene expression pattern in swine neutrophils after lipopolysaccharide exposure: a time course comparison
title_full_unstemmed Gene expression pattern in swine neutrophils after lipopolysaccharide exposure: a time course comparison
title_short Gene expression pattern in swine neutrophils after lipopolysaccharide exposure: a time course comparison
title_sort gene expression pattern in swine neutrophils after lipopolysaccharide exposure: a time course comparison
topic Proceedings
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3108205/
https://www.ncbi.nlm.nih.gov/pubmed/21645290
http://dx.doi.org/10.1186/1753-6561-5-S4-S11
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