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Expression of trypanotolerance in N’Dama x Boran crosses under field challenge in relation to N’Dama genome content

BACKGROUND: Animal trypanosomosis in sub-Saharan Africa is a major obstacle to livestock based agriculture. Control relies on drugs with increasing incidence of multiple-drug resistance. A previous mapping experiment in an F2 population derived from the indigenous trypanotolerant N’Dama cattle cross...

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Autores principales: Orenge, Caleb, Munga, Leonard, Kimwele, Charles, Kemp, Steve, Korol, Abraham, Gibson, John, Hanotte, Olivier, Soller, Morris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3108218/
https://www.ncbi.nlm.nih.gov/pubmed/21645303
http://dx.doi.org/10.1186/1753-6561-5-S4-S23
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author Orenge, Caleb
Munga, Leonard
Kimwele, Charles
Kemp, Steve
Korol, Abraham
Gibson, John
Hanotte, Olivier
Soller, Morris
author_facet Orenge, Caleb
Munga, Leonard
Kimwele, Charles
Kemp, Steve
Korol, Abraham
Gibson, John
Hanotte, Olivier
Soller, Morris
author_sort Orenge, Caleb
collection PubMed
description BACKGROUND: Animal trypanosomosis in sub-Saharan Africa is a major obstacle to livestock based agriculture. Control relies on drugs with increasing incidence of multiple-drug resistance. A previous mapping experiment in an F2 population derived from the indigenous trypanotolerant N’Dama cattle crossed to susceptible (Kenya)-Boran cattle under controlled challenge, uncovered a number of trypanotolerance QTL (T-QTL). The present study was to determine expression of N’Dama trypanotolerance in a backcross to the Boran under conditions of field challenge, and whether chromosomal regions associated with trypanotolerance in the F2 experiment showed similar effects in the BC population. METHODS: 192 backcross animals to the Boran were produced in six batches from June 2001 to December 2006. At one year of age animals were moved to the field and exposed to natural challenge over about one year in Southwest Kenya (Narok). The animals were individually recorded weekly for body weight, packed cell volume, parasitaemia score, and drug treatments, and were genotyped using 35 microsatellite markers spanning 5 chromosomes found in the F2 study to harbour T-QTL. RESULTS: The F1 were most trypanotolerant, Boran least, and BC intermediate. Females showed distinctly higher trypanotolerance than males. There was a positive correlation in the BC population between trypanotolerance and number of N’Dama origin marker alleles. QTL mapping revealed T-QTL distributed among all five targeted chromosomes, corresponding in part to the results obtained in the F(2) experiment. CONCLUSIONS: N’Dama origin trypanotolerance is expressed in a BC population under field conditions in proportion to N’Dama origin marker alleles. Consequently, marker assisted selection in such populations may be a means of increasing trypanotolerance, while retaining the desirable productive qualities of the recurrent parent.
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spelling pubmed-31082182011-06-07 Expression of trypanotolerance in N’Dama x Boran crosses under field challenge in relation to N’Dama genome content Orenge, Caleb Munga, Leonard Kimwele, Charles Kemp, Steve Korol, Abraham Gibson, John Hanotte, Olivier Soller, Morris BMC Proc Proceedings BACKGROUND: Animal trypanosomosis in sub-Saharan Africa is a major obstacle to livestock based agriculture. Control relies on drugs with increasing incidence of multiple-drug resistance. A previous mapping experiment in an F2 population derived from the indigenous trypanotolerant N’Dama cattle crossed to susceptible (Kenya)-Boran cattle under controlled challenge, uncovered a number of trypanotolerance QTL (T-QTL). The present study was to determine expression of N’Dama trypanotolerance in a backcross to the Boran under conditions of field challenge, and whether chromosomal regions associated with trypanotolerance in the F2 experiment showed similar effects in the BC population. METHODS: 192 backcross animals to the Boran were produced in six batches from June 2001 to December 2006. At one year of age animals were moved to the field and exposed to natural challenge over about one year in Southwest Kenya (Narok). The animals were individually recorded weekly for body weight, packed cell volume, parasitaemia score, and drug treatments, and were genotyped using 35 microsatellite markers spanning 5 chromosomes found in the F2 study to harbour T-QTL. RESULTS: The F1 were most trypanotolerant, Boran least, and BC intermediate. Females showed distinctly higher trypanotolerance than males. There was a positive correlation in the BC population between trypanotolerance and number of N’Dama origin marker alleles. QTL mapping revealed T-QTL distributed among all five targeted chromosomes, corresponding in part to the results obtained in the F(2) experiment. CONCLUSIONS: N’Dama origin trypanotolerance is expressed in a BC population under field conditions in proportion to N’Dama origin marker alleles. Consequently, marker assisted selection in such populations may be a means of increasing trypanotolerance, while retaining the desirable productive qualities of the recurrent parent. BioMed Central 2011-06-03 /pmc/articles/PMC3108218/ /pubmed/21645303 http://dx.doi.org/10.1186/1753-6561-5-S4-S23 Text en Copyright ©2011 Orenge et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Proceedings
Orenge, Caleb
Munga, Leonard
Kimwele, Charles
Kemp, Steve
Korol, Abraham
Gibson, John
Hanotte, Olivier
Soller, Morris
Expression of trypanotolerance in N’Dama x Boran crosses under field challenge in relation to N’Dama genome content
title Expression of trypanotolerance in N’Dama x Boran crosses under field challenge in relation to N’Dama genome content
title_full Expression of trypanotolerance in N’Dama x Boran crosses under field challenge in relation to N’Dama genome content
title_fullStr Expression of trypanotolerance in N’Dama x Boran crosses under field challenge in relation to N’Dama genome content
title_full_unstemmed Expression of trypanotolerance in N’Dama x Boran crosses under field challenge in relation to N’Dama genome content
title_short Expression of trypanotolerance in N’Dama x Boran crosses under field challenge in relation to N’Dama genome content
title_sort expression of trypanotolerance in n’dama x boran crosses under field challenge in relation to n’dama genome content
topic Proceedings
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3108218/
https://www.ncbi.nlm.nih.gov/pubmed/21645303
http://dx.doi.org/10.1186/1753-6561-5-S4-S23
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