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Genomic study of the response of chicken to highly pathogenic avian influenza virus
BACKGROUND: The host mounts an immune response to pathogens, but few data are currently available on the role of host genetics in variation in response to avian influenza (AI). The study presented here investigated the role of the host genetic background in response to in vivo infection with AI viru...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3108220/ https://www.ncbi.nlm.nih.gov/pubmed/21645305 http://dx.doi.org/10.1186/1753-6561-5-S4-S25 |
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author | Sironi, Laura Williams, John L Stella, Alessandra Minozzi, Giulietta Moreno, Ana Ramelli, Paola Han, Jianlin Weigend, Steffen Wan, Junxing Lombardi, Guerino Cordioli, Paolo Mariani, Paola |
author_facet | Sironi, Laura Williams, John L Stella, Alessandra Minozzi, Giulietta Moreno, Ana Ramelli, Paola Han, Jianlin Weigend, Steffen Wan, Junxing Lombardi, Guerino Cordioli, Paolo Mariani, Paola |
author_sort | Sironi, Laura |
collection | PubMed |
description | BACKGROUND: The host mounts an immune response to pathogens, but few data are currently available on the role of host genetics in variation in response to avian influenza (AI). The study presented here investigated the role of the host genetic background in response to in vivo infection with AI virus (AIV). METHODS: Experimental lines of chicken and commercial crosses were experimentally infected intratracheally with 10(3) EID(50)/bird of A/Chicken/Italy/13474/99 H7N1 highly pathogenic avian influenza virus (HPAIV). Chickens were genotyped for the Mx polymorphism causing the S631N mutation, and for the Major Histocompatibility Complex (MHC). Whole-genome genotyping was carried out using 60 k Single Nucleotide Polymorphism (SNP) array developed by the poultry Genome-Wide Marker-Assisted Selection Consortium (GWMASC). RESULTS: Variability in response of different chicken lines to the HPAIV infections and some degree of resistance to AI were observed: a statistically significant effect of chicken line on the response to infection was found. There was no association between survival in healthy conditions and polymorphisms at the Mx gene and the MHC-B region. The analysis based on the 60 k SNPs provided a good clustering of the chicken lines, but no specific genetic cluster associated with response to AIV was identified. CONCLUSIONS: Neither the genotype at the Mx gene or MHC-B locus, nor for SNP spanning the whole-genome identified loci involved in variations to response to AIV infection. These results point towards the possibility that either the genetic factors affecting the response of chickens to the H7N1 HPAIV are weak, or relevant alleles were not segregating in the studied populations. |
format | Online Article Text |
id | pubmed-3108220 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-31082202011-06-07 Genomic study of the response of chicken to highly pathogenic avian influenza virus Sironi, Laura Williams, John L Stella, Alessandra Minozzi, Giulietta Moreno, Ana Ramelli, Paola Han, Jianlin Weigend, Steffen Wan, Junxing Lombardi, Guerino Cordioli, Paolo Mariani, Paola BMC Proc Proceedings BACKGROUND: The host mounts an immune response to pathogens, but few data are currently available on the role of host genetics in variation in response to avian influenza (AI). The study presented here investigated the role of the host genetic background in response to in vivo infection with AI virus (AIV). METHODS: Experimental lines of chicken and commercial crosses were experimentally infected intratracheally with 10(3) EID(50)/bird of A/Chicken/Italy/13474/99 H7N1 highly pathogenic avian influenza virus (HPAIV). Chickens were genotyped for the Mx polymorphism causing the S631N mutation, and for the Major Histocompatibility Complex (MHC). Whole-genome genotyping was carried out using 60 k Single Nucleotide Polymorphism (SNP) array developed by the poultry Genome-Wide Marker-Assisted Selection Consortium (GWMASC). RESULTS: Variability in response of different chicken lines to the HPAIV infections and some degree of resistance to AI were observed: a statistically significant effect of chicken line on the response to infection was found. There was no association between survival in healthy conditions and polymorphisms at the Mx gene and the MHC-B region. The analysis based on the 60 k SNPs provided a good clustering of the chicken lines, but no specific genetic cluster associated with response to AIV was identified. CONCLUSIONS: Neither the genotype at the Mx gene or MHC-B locus, nor for SNP spanning the whole-genome identified loci involved in variations to response to AIV infection. These results point towards the possibility that either the genetic factors affecting the response of chickens to the H7N1 HPAIV are weak, or relevant alleles were not segregating in the studied populations. BioMed Central 2011-06-03 /pmc/articles/PMC3108220/ /pubmed/21645305 http://dx.doi.org/10.1186/1753-6561-5-S4-S25 Text en Copyright ©2011 Sironi et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Proceedings Sironi, Laura Williams, John L Stella, Alessandra Minozzi, Giulietta Moreno, Ana Ramelli, Paola Han, Jianlin Weigend, Steffen Wan, Junxing Lombardi, Guerino Cordioli, Paolo Mariani, Paola Genomic study of the response of chicken to highly pathogenic avian influenza virus |
title | Genomic study of the response of chicken to highly pathogenic avian influenza virus |
title_full | Genomic study of the response of chicken to highly pathogenic avian influenza virus |
title_fullStr | Genomic study of the response of chicken to highly pathogenic avian influenza virus |
title_full_unstemmed | Genomic study of the response of chicken to highly pathogenic avian influenza virus |
title_short | Genomic study of the response of chicken to highly pathogenic avian influenza virus |
title_sort | genomic study of the response of chicken to highly pathogenic avian influenza virus |
topic | Proceedings |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3108220/ https://www.ncbi.nlm.nih.gov/pubmed/21645305 http://dx.doi.org/10.1186/1753-6561-5-S4-S25 |
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