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Detection and differentiation of Borrelia burgdorferi sensu lato in ticks collected from sheep and cattle in China

BACKGROUND: Lyme disease caused by Borrelia burgdorferi sensu lato complex is an important endemic zoonosis whose distribution is closely related to the main ixodid tick vectors. In China, isolated cases of Lyme disease infection of humans have been reported in 29 provinces. Ticks, especially ixodid...

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Autores principales: Niu, Qingli, Guan, Guiquan, Yang, Jifei, Fu, Yuguang, Xu, Zongke, Li, Youquan, Ma, Miling, Liu, Zhijie, Liu, Junlong, Liu, Aihong, Ren, Qiaoyun, Jorgensen, Wayne, Luo, Jianxun, Yin, Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3108939/
https://www.ncbi.nlm.nih.gov/pubmed/21529349
http://dx.doi.org/10.1186/1746-6148-7-17
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author Niu, Qingli
Guan, Guiquan
Yang, Jifei
Fu, Yuguang
Xu, Zongke
Li, Youquan
Ma, Miling
Liu, Zhijie
Liu, Junlong
Liu, Aihong
Ren, Qiaoyun
Jorgensen, Wayne
Luo, Jianxun
Yin, Hong
author_facet Niu, Qingli
Guan, Guiquan
Yang, Jifei
Fu, Yuguang
Xu, Zongke
Li, Youquan
Ma, Miling
Liu, Zhijie
Liu, Junlong
Liu, Aihong
Ren, Qiaoyun
Jorgensen, Wayne
Luo, Jianxun
Yin, Hong
author_sort Niu, Qingli
collection PubMed
description BACKGROUND: Lyme disease caused by Borrelia burgdorferi sensu lato complex is an important endemic zoonosis whose distribution is closely related to the main ixodid tick vectors. In China, isolated cases of Lyme disease infection of humans have been reported in 29 provinces. Ticks, especially ixodid ticks are abundant and a wide arrange of Borrelia natural reservoirs are present. In this study, we developed a reverse line blot (RLB) to identify Borrelia spp. in ticks collected from sheep and cattle in 7 Provinces covering the main extensive livestock regions in China. RESULTS: Four species-specific RLB oligonucleotide probes were deduced from the spacer region between the 5S-23S rRNA gene, along with an oligonucleotide probe which was common to all. The species specific probes were shown to discriminate between four genomic groups of B. burgdorferi sensu lato i.e. B. burgdorferi sensu stricto, B. garinii, B. afzelii, and B. valaisiana, and to bind only to their respective target sequences, with no cross reaction to non target DNA. Furthermore, the RLB could detect between 0.1 pg and 1 pg of Borrelia DNA. A total of 723 tick samples (Haemaphysalis, Boophilus, Rhipicephalus and Dermacentor) from sheep and cattle were examined with RLB, and a subset of 667 corresponding samples were examined with PCR as a comparison. The overall infection rate detected with RLB was higher than that of the PCR test. The infection rate of B. burgdoreri sensu stricto was 40% in south areas; while the B. garinii infection rate was 40% in north areas. The highest detection rates of B. afzelii and B. valaisiana were 28% and 22%, respectively. Mixed infections were also found in 7% of the ticks analyzed, mainly in the North. The proportion of B. garinii genotype in ticks was overall highest at 34% in the whole investigation area. CONCLUSION: In this study, the RLB assay was used to detect B. burgdorferi sensu lato in ticks collected from sheep and cattle in China. The results showed that B. burdorferi senso stricto and B. afzelii were mainly distributed in the South; while B. garinii and B. valaisiana were dominant in the North. Borrelia spirochaetes were detected in Rhipicephalus spp for the first time. It is suggested that the Rhipicephalus spps might play a role in transmitting Borrelia spirochaetes.
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spelling pubmed-31089392011-06-07 Detection and differentiation of Borrelia burgdorferi sensu lato in ticks collected from sheep and cattle in China Niu, Qingli Guan, Guiquan Yang, Jifei Fu, Yuguang Xu, Zongke Li, Youquan Ma, Miling Liu, Zhijie Liu, Junlong Liu, Aihong Ren, Qiaoyun Jorgensen, Wayne Luo, Jianxun Yin, Hong BMC Vet Res Research Article BACKGROUND: Lyme disease caused by Borrelia burgdorferi sensu lato complex is an important endemic zoonosis whose distribution is closely related to the main ixodid tick vectors. In China, isolated cases of Lyme disease infection of humans have been reported in 29 provinces. Ticks, especially ixodid ticks are abundant and a wide arrange of Borrelia natural reservoirs are present. In this study, we developed a reverse line blot (RLB) to identify Borrelia spp. in ticks collected from sheep and cattle in 7 Provinces covering the main extensive livestock regions in China. RESULTS: Four species-specific RLB oligonucleotide probes were deduced from the spacer region between the 5S-23S rRNA gene, along with an oligonucleotide probe which was common to all. The species specific probes were shown to discriminate between four genomic groups of B. burgdorferi sensu lato i.e. B. burgdorferi sensu stricto, B. garinii, B. afzelii, and B. valaisiana, and to bind only to their respective target sequences, with no cross reaction to non target DNA. Furthermore, the RLB could detect between 0.1 pg and 1 pg of Borrelia DNA. A total of 723 tick samples (Haemaphysalis, Boophilus, Rhipicephalus and Dermacentor) from sheep and cattle were examined with RLB, and a subset of 667 corresponding samples were examined with PCR as a comparison. The overall infection rate detected with RLB was higher than that of the PCR test. The infection rate of B. burgdoreri sensu stricto was 40% in south areas; while the B. garinii infection rate was 40% in north areas. The highest detection rates of B. afzelii and B. valaisiana were 28% and 22%, respectively. Mixed infections were also found in 7% of the ticks analyzed, mainly in the North. The proportion of B. garinii genotype in ticks was overall highest at 34% in the whole investigation area. CONCLUSION: In this study, the RLB assay was used to detect B. burgdorferi sensu lato in ticks collected from sheep and cattle in China. The results showed that B. burdorferi senso stricto and B. afzelii were mainly distributed in the South; while B. garinii and B. valaisiana were dominant in the North. Borrelia spirochaetes were detected in Rhipicephalus spp for the first time. It is suggested that the Rhipicephalus spps might play a role in transmitting Borrelia spirochaetes. BioMed Central 2011-04-29 /pmc/articles/PMC3108939/ /pubmed/21529349 http://dx.doi.org/10.1186/1746-6148-7-17 Text en Copyright ©2011 Niu et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Niu, Qingli
Guan, Guiquan
Yang, Jifei
Fu, Yuguang
Xu, Zongke
Li, Youquan
Ma, Miling
Liu, Zhijie
Liu, Junlong
Liu, Aihong
Ren, Qiaoyun
Jorgensen, Wayne
Luo, Jianxun
Yin, Hong
Detection and differentiation of Borrelia burgdorferi sensu lato in ticks collected from sheep and cattle in China
title Detection and differentiation of Borrelia burgdorferi sensu lato in ticks collected from sheep and cattle in China
title_full Detection and differentiation of Borrelia burgdorferi sensu lato in ticks collected from sheep and cattle in China
title_fullStr Detection and differentiation of Borrelia burgdorferi sensu lato in ticks collected from sheep and cattle in China
title_full_unstemmed Detection and differentiation of Borrelia burgdorferi sensu lato in ticks collected from sheep and cattle in China
title_short Detection and differentiation of Borrelia burgdorferi sensu lato in ticks collected from sheep and cattle in China
title_sort detection and differentiation of borrelia burgdorferi sensu lato in ticks collected from sheep and cattle in china
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3108939/
https://www.ncbi.nlm.nih.gov/pubmed/21529349
http://dx.doi.org/10.1186/1746-6148-7-17
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