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Expression of EGFR and Microvessel Density in Middle Ear Cholesteatoma

OBJECTIVES: Cholesteatoma destructs bony tissue by the interactions between hyperproliferative epithelial cells and subepithelial inflammatory cells. The aim of this study was to evaluate the expression of epidermal growth factor receptor (EGFR) and microvessel density (MVD) in middle ear cholesteat...

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Autores principales: Jin, Bong Joon, Min, Hyun Jung, Jeong, Jin Hyeok, Park, Chul Won, Lee, Seung Hwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Otorhinolaryngology-Head and Neck Surgery 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3109329/
https://www.ncbi.nlm.nih.gov/pubmed/21716952
http://dx.doi.org/10.3342/ceo.2011.4.2.67
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author Jin, Bong Joon
Min, Hyun Jung
Jeong, Jin Hyeok
Park, Chul Won
Lee, Seung Hwan
author_facet Jin, Bong Joon
Min, Hyun Jung
Jeong, Jin Hyeok
Park, Chul Won
Lee, Seung Hwan
author_sort Jin, Bong Joon
collection PubMed
description OBJECTIVES: Cholesteatoma destructs bony tissue by the interactions between hyperproliferative epithelial cells and subepithelial inflammatory cells. The aim of this study was to evaluate the expression of epidermal growth factor receptor (EGFR) and microvessel density (MVD) in middle ear cholesteatoma tissue in an effort to determine the relationship between expression of EGFR and neovascularization. METHODS: We evaluated the expression of EGFR and MVD by immunohistochemical staining for CD31 and Factor VIII in 32 cholesteatoma tissue samples and 7 normal postauricular skin samples. We also analyzed the correlation between EGFR expression and MVD. RESULTS: The expression of EGFR was higher in cholesteatoma than in postauricular skin, but the difference was not statistically significant. EGFR was more highly expressed in the suprabasal layer than in the basal layer. Using CD31 and Factor VIII, we analyzed the MVD and found that it was significantly higher in cholesteatoma than in postauricular skin, and significantly correlated with the expression of EGFR. CONCLUSION: Our results suggest that overexpression of EGFR and neovascularization are correlated with the growth of cholesteatoma.
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spelling pubmed-31093292011-06-28 Expression of EGFR and Microvessel Density in Middle Ear Cholesteatoma Jin, Bong Joon Min, Hyun Jung Jeong, Jin Hyeok Park, Chul Won Lee, Seung Hwan Clin Exp Otorhinolaryngol Original Article OBJECTIVES: Cholesteatoma destructs bony tissue by the interactions between hyperproliferative epithelial cells and subepithelial inflammatory cells. The aim of this study was to evaluate the expression of epidermal growth factor receptor (EGFR) and microvessel density (MVD) in middle ear cholesteatoma tissue in an effort to determine the relationship between expression of EGFR and neovascularization. METHODS: We evaluated the expression of EGFR and MVD by immunohistochemical staining for CD31 and Factor VIII in 32 cholesteatoma tissue samples and 7 normal postauricular skin samples. We also analyzed the correlation between EGFR expression and MVD. RESULTS: The expression of EGFR was higher in cholesteatoma than in postauricular skin, but the difference was not statistically significant. EGFR was more highly expressed in the suprabasal layer than in the basal layer. Using CD31 and Factor VIII, we analyzed the MVD and found that it was significantly higher in cholesteatoma than in postauricular skin, and significantly correlated with the expression of EGFR. CONCLUSION: Our results suggest that overexpression of EGFR and neovascularization are correlated with the growth of cholesteatoma. Korean Society of Otorhinolaryngology-Head and Neck Surgery 2011-06 2011-05-31 /pmc/articles/PMC3109329/ /pubmed/21716952 http://dx.doi.org/10.3342/ceo.2011.4.2.67 Text en Copyright © 2011 by Korean Society of Otorhinolaryngology-Head and Neck Surgery. http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Jin, Bong Joon
Min, Hyun Jung
Jeong, Jin Hyeok
Park, Chul Won
Lee, Seung Hwan
Expression of EGFR and Microvessel Density in Middle Ear Cholesteatoma
title Expression of EGFR and Microvessel Density in Middle Ear Cholesteatoma
title_full Expression of EGFR and Microvessel Density in Middle Ear Cholesteatoma
title_fullStr Expression of EGFR and Microvessel Density in Middle Ear Cholesteatoma
title_full_unstemmed Expression of EGFR and Microvessel Density in Middle Ear Cholesteatoma
title_short Expression of EGFR and Microvessel Density in Middle Ear Cholesteatoma
title_sort expression of egfr and microvessel density in middle ear cholesteatoma
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3109329/
https://www.ncbi.nlm.nih.gov/pubmed/21716952
http://dx.doi.org/10.3342/ceo.2011.4.2.67
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