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Chronic consumption of distilled sugarcane spirit induces anxiolytic-like effects in mice

OBJECTIVE: Chronic ethanol consumption is a major public health problem throughout the world. We investigated the anxiolytic-like effects and the possible ever injury induced by the chronic consumption of ethanol or sugarcane spirit in mice. METHOD: Adult mice were exposed to a two-bottle free-choic...

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Autores principales: Sena, Maria Clécia P, Nunes, Fabíola C, Stiebbe Salvadori, Mirian G S, Carvalho, Cleyton Charles D, Morais, Liana Clébia S L, Braga, Valdir A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3109389/
https://www.ncbi.nlm.nih.gov/pubmed/21789394
http://dx.doi.org/10.1590/S1807-59322011000500026
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author Sena, Maria Clécia P
Nunes, Fabíola C
Stiebbe Salvadori, Mirian G S
Carvalho, Cleyton Charles D
Morais, Liana Clébia S L
Braga, Valdir A
author_facet Sena, Maria Clécia P
Nunes, Fabíola C
Stiebbe Salvadori, Mirian G S
Carvalho, Cleyton Charles D
Morais, Liana Clébia S L
Braga, Valdir A
author_sort Sena, Maria Clécia P
collection PubMed
description OBJECTIVE: Chronic ethanol consumption is a major public health problem throughout the world. We investigated the anxiolytic-like effects and the possible ever injury induced by the chronic consumption of ethanol or sugarcane spirit in mice. METHOD: Adult mice were exposed to a two-bottle free-choice paradigm for 6 weeks. The mice in Group A (n  =  16) had access to sugarcane spirit + distilled water, the mice in Group B (n  =  15) had access to ethanol + distilled water, and the mice in Group C (control, n  =  14) had access to distilled water + distilled water. The ethanol content in the beverages offered to Groups A and B was 2% for the first week, 5% for the second week and 10% for the remaining four weeks. At the end of the experimental period, the mice were evaluated using the elevated-plus maze and the hole-board test to assess their anxiety-related behaviors. We also determined the serum aspartate aminotransferase and alanine aminotransferase levels. RESULTS: In the elevated-plus maze, the time spent in the open arms was increased in the mice exposed to chronic ethanol (32 ± 8 vs. 7 ± 2 s, n  =  9) or sugarcane spirit (36 ± 9 vs. 7 ± 2 s, n  =  9) compared to the controls. In the hole-board test, the mice exposed to ethanol or sugarcane spirit displayed increases in their head-dipping frequency (16 ± 1 for the control group, 27 ± 2 for the ethanol group, and 31 ± 3 for the sugarcane-spirit group; n  =  9 for each group). In addition, the mice exposed to sugarcane spirit displayed an increase in the aspartate aminotransferase / alanine aminotransferase ratio compared to the ethanol group (1.29 ± 0.17 for the control group and 2.67 ± 0.17 for the sugarcane spirit group; n  =  8 for each group). CONCLUSION: The chronic consumption of sugarcane-spirit produces liver injury and anxiolytic-like effects and the possible liver injury in mice.
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spelling pubmed-31093892011-06-07 Chronic consumption of distilled sugarcane spirit induces anxiolytic-like effects in mice Sena, Maria Clécia P Nunes, Fabíola C Stiebbe Salvadori, Mirian G S Carvalho, Cleyton Charles D Morais, Liana Clébia S L Braga, Valdir A Clinics (Sao Paulo) Basic Research OBJECTIVE: Chronic ethanol consumption is a major public health problem throughout the world. We investigated the anxiolytic-like effects and the possible ever injury induced by the chronic consumption of ethanol or sugarcane spirit in mice. METHOD: Adult mice were exposed to a two-bottle free-choice paradigm for 6 weeks. The mice in Group A (n  =  16) had access to sugarcane spirit + distilled water, the mice in Group B (n  =  15) had access to ethanol + distilled water, and the mice in Group C (control, n  =  14) had access to distilled water + distilled water. The ethanol content in the beverages offered to Groups A and B was 2% for the first week, 5% for the second week and 10% for the remaining four weeks. At the end of the experimental period, the mice were evaluated using the elevated-plus maze and the hole-board test to assess their anxiety-related behaviors. We also determined the serum aspartate aminotransferase and alanine aminotransferase levels. RESULTS: In the elevated-plus maze, the time spent in the open arms was increased in the mice exposed to chronic ethanol (32 ± 8 vs. 7 ± 2 s, n  =  9) or sugarcane spirit (36 ± 9 vs. 7 ± 2 s, n  =  9) compared to the controls. In the hole-board test, the mice exposed to ethanol or sugarcane spirit displayed increases in their head-dipping frequency (16 ± 1 for the control group, 27 ± 2 for the ethanol group, and 31 ± 3 for the sugarcane-spirit group; n  =  9 for each group). In addition, the mice exposed to sugarcane spirit displayed an increase in the aspartate aminotransferase / alanine aminotransferase ratio compared to the ethanol group (1.29 ± 0.17 for the control group and 2.67 ± 0.17 for the sugarcane spirit group; n  =  8 for each group). CONCLUSION: The chronic consumption of sugarcane-spirit produces liver injury and anxiolytic-like effects and the possible liver injury in mice. Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo 2011-05 /pmc/articles/PMC3109389/ /pubmed/21789394 http://dx.doi.org/10.1590/S1807-59322011000500026 Text en Copyright © 2011 Hospital das Clínicas da FMUSP http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Basic Research
Sena, Maria Clécia P
Nunes, Fabíola C
Stiebbe Salvadori, Mirian G S
Carvalho, Cleyton Charles D
Morais, Liana Clébia S L
Braga, Valdir A
Chronic consumption of distilled sugarcane spirit induces anxiolytic-like effects in mice
title Chronic consumption of distilled sugarcane spirit induces anxiolytic-like effects in mice
title_full Chronic consumption of distilled sugarcane spirit induces anxiolytic-like effects in mice
title_fullStr Chronic consumption of distilled sugarcane spirit induces anxiolytic-like effects in mice
title_full_unstemmed Chronic consumption of distilled sugarcane spirit induces anxiolytic-like effects in mice
title_short Chronic consumption of distilled sugarcane spirit induces anxiolytic-like effects in mice
title_sort chronic consumption of distilled sugarcane spirit induces anxiolytic-like effects in mice
topic Basic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3109389/
https://www.ncbi.nlm.nih.gov/pubmed/21789394
http://dx.doi.org/10.1590/S1807-59322011000500026
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