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Induction and Flow Cytometry Identification of Tetraploids from Seed-Derived Explants through Colchicine Treatments in Catharanthus roseus (L.) G. Don
The tetraploid plants of Catharanthus roseus (L.) G. Don was obtained by colchicine induction from seeds explants, and the ploidy of the plants was identified by flow cytometry. The optimal treatment is 0.2% colchicine solution treated for 24 hours, and the induction rate reaches up to 30%. Comparin...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3110335/ https://www.ncbi.nlm.nih.gov/pubmed/21660143 http://dx.doi.org/10.1155/2011/793198 |
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author | Xing, Shi-Hai Guo, Xin-Bo Wang, Quan Pan, Qi-Fang Tian, Yue-Sheng Liu, Pin Zhao, Jing-Ya Wang, Guo-Feng Sun, Xiao-Fen Tang, Ke-Xuan |
author_facet | Xing, Shi-Hai Guo, Xin-Bo Wang, Quan Pan, Qi-Fang Tian, Yue-Sheng Liu, Pin Zhao, Jing-Ya Wang, Guo-Feng Sun, Xiao-Fen Tang, Ke-Xuan |
author_sort | Xing, Shi-Hai |
collection | PubMed |
description | The tetraploid plants of Catharanthus roseus (L.) G. Don was obtained by colchicine induction from seeds explants, and the ploidy of the plants was identified by flow cytometry. The optimal treatment is 0.2% colchicine solution treated for 24 hours, and the induction rate reaches up to 30%. Comparing with morphological characteristics and growth habits between tetraploids and the control, we found that tetraploids of C. roseus had larger stoma and more branches and leaves. HPLC analysis showed tetraploidization could increase the contents of terpenoid indole alkaloids in C. roseus. Thus, tetraploidization could be used to produce higher alkaloids lines for commercial use. QRT-PCR results showed that the expression of enzymes involved in terpenoid indole alkaloids biosynthesis pathway had increased in the tetraploid plants. To our knowledge, this was the first paper to explore the secondary metabolism in autotetraploid C. roseus induced by colchicine. |
format | Online Article Text |
id | pubmed-3110335 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-31103352011-06-09 Induction and Flow Cytometry Identification of Tetraploids from Seed-Derived Explants through Colchicine Treatments in Catharanthus roseus (L.) G. Don Xing, Shi-Hai Guo, Xin-Bo Wang, Quan Pan, Qi-Fang Tian, Yue-Sheng Liu, Pin Zhao, Jing-Ya Wang, Guo-Feng Sun, Xiao-Fen Tang, Ke-Xuan J Biomed Biotechnol Research Article The tetraploid plants of Catharanthus roseus (L.) G. Don was obtained by colchicine induction from seeds explants, and the ploidy of the plants was identified by flow cytometry. The optimal treatment is 0.2% colchicine solution treated for 24 hours, and the induction rate reaches up to 30%. Comparing with morphological characteristics and growth habits between tetraploids and the control, we found that tetraploids of C. roseus had larger stoma and more branches and leaves. HPLC analysis showed tetraploidization could increase the contents of terpenoid indole alkaloids in C. roseus. Thus, tetraploidization could be used to produce higher alkaloids lines for commercial use. QRT-PCR results showed that the expression of enzymes involved in terpenoid indole alkaloids biosynthesis pathway had increased in the tetraploid plants. To our knowledge, this was the first paper to explore the secondary metabolism in autotetraploid C. roseus induced by colchicine. Hindawi Publishing Corporation 2011 2011-05-29 /pmc/articles/PMC3110335/ /pubmed/21660143 http://dx.doi.org/10.1155/2011/793198 Text en Copyright © 2011 Shi-Hai Xing et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Xing, Shi-Hai Guo, Xin-Bo Wang, Quan Pan, Qi-Fang Tian, Yue-Sheng Liu, Pin Zhao, Jing-Ya Wang, Guo-Feng Sun, Xiao-Fen Tang, Ke-Xuan Induction and Flow Cytometry Identification of Tetraploids from Seed-Derived Explants through Colchicine Treatments in Catharanthus roseus (L.) G. Don |
title | Induction and Flow Cytometry Identification of Tetraploids from Seed-Derived Explants through Colchicine Treatments in Catharanthus roseus (L.) G. Don |
title_full | Induction and Flow Cytometry Identification of Tetraploids from Seed-Derived Explants through Colchicine Treatments in Catharanthus roseus (L.) G. Don |
title_fullStr | Induction and Flow Cytometry Identification of Tetraploids from Seed-Derived Explants through Colchicine Treatments in Catharanthus roseus (L.) G. Don |
title_full_unstemmed | Induction and Flow Cytometry Identification of Tetraploids from Seed-Derived Explants through Colchicine Treatments in Catharanthus roseus (L.) G. Don |
title_short | Induction and Flow Cytometry Identification of Tetraploids from Seed-Derived Explants through Colchicine Treatments in Catharanthus roseus (L.) G. Don |
title_sort | induction and flow cytometry identification of tetraploids from seed-derived explants through colchicine treatments in catharanthus roseus (l.) g. don |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3110335/ https://www.ncbi.nlm.nih.gov/pubmed/21660143 http://dx.doi.org/10.1155/2011/793198 |
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