Preparation and Fractionation of Xenopus laevis Egg Extracts

Crude and fractionated Xenopus egg extracts can be used to provide ingredients for reconstituting cellular processes for morphological and biochemical analysis. Egg lysis and differential centrifugation are used to prepare the crude extract which in turn in used to prepare fractionated extracts and...

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Detalles Bibliográficos
Autores principales: K. Cross, Marie, Powers, Maureen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2008
Materias:
Cellular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3110484/
https://www.ncbi.nlm.nih.gov/pubmed/19066500
http://dx.doi.org/10.3791/891
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author K. Cross, Marie
Powers, Maureen
author_facet K. Cross, Marie
Powers, Maureen
author_sort K. Cross, Marie
collection PubMed
description Crude and fractionated Xenopus egg extracts can be used to provide ingredients for reconstituting cellular processes for morphological and biochemical analysis. Egg lysis and differential centrifugation are used to prepare the crude extract which in turn in used to prepare fractionated extracts and light membrane preparations.
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spelling pubmed-31104842011-07-07 Preparation and Fractionation of Xenopus laevis Egg Extracts K. Cross, Marie Powers, Maureen J Vis Exp Cellular Biology Crude and fractionated Xenopus egg extracts can be used to provide ingredients for reconstituting cellular processes for morphological and biochemical analysis. Egg lysis and differential centrifugation are used to prepare the crude extract which in turn in used to prepare fractionated extracts and light membrane preparations. MyJove Corporation 2008-08-27 /pmc/articles/PMC3110484/ /pubmed/19066500 http://dx.doi.org/10.3791/891 Text en Copyright © 2008, Journal of Visualized Experiments http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Cellular Biology
K. Cross, Marie
Powers, Maureen
Preparation and Fractionation of Xenopus laevis Egg Extracts
title Preparation and Fractionation of Xenopus laevis Egg Extracts
title_full Preparation and Fractionation of Xenopus laevis Egg Extracts
title_fullStr Preparation and Fractionation of Xenopus laevis Egg Extracts
title_full_unstemmed Preparation and Fractionation of Xenopus laevis Egg Extracts
title_short Preparation and Fractionation of Xenopus laevis Egg Extracts
title_sort preparation and fractionation of xenopus laevis egg extracts
topic Cellular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3110484/
https://www.ncbi.nlm.nih.gov/pubmed/19066500
http://dx.doi.org/10.3791/891
work_keys_str_mv AT kcrossmarie preparationandfractionationofxenopuslaeviseggextracts
AT powersmaureen preparationandfractionationofxenopuslaeviseggextracts

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