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Diagnostic Value of a Chimeric TSH Receptor (Mc4)-Based Bioassay for Graves' Disease

BACKGROUND/AIMS: Graves' disease (GD) is caused by thyroid-stimulating hormone receptor (TSHR) and thyroid-stimulating immunoglobulin (TSI). We used a recently introduced, technically enhanced TSI bioassay to assess its diagnostic value and determine the cut-off in patients in high iodine intak...

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Autores principales: Lee, Ji In, Jang, Hye Won, Kim, Soo Kyoung, Choi, Joon Young, Kim, Ji Young, Hur, Kyu Yeon, Kim, Jae Hyeon, Min, Yong-Ki, Chung, Jae Hoon, Kim, Sun Wook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Association of Internal Medicine 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3110850/
https://www.ncbi.nlm.nih.gov/pubmed/21716594
http://dx.doi.org/10.3904/kjim.2011.26.2.179
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author Lee, Ji In
Jang, Hye Won
Kim, Soo Kyoung
Choi, Joon Young
Kim, Ji Young
Hur, Kyu Yeon
Kim, Jae Hyeon
Min, Yong-Ki
Chung, Jae Hoon
Kim, Sun Wook
author_facet Lee, Ji In
Jang, Hye Won
Kim, Soo Kyoung
Choi, Joon Young
Kim, Ji Young
Hur, Kyu Yeon
Kim, Jae Hyeon
Min, Yong-Ki
Chung, Jae Hoon
Kim, Sun Wook
author_sort Lee, Ji In
collection PubMed
description BACKGROUND/AIMS: Graves' disease (GD) is caused by thyroid-stimulating hormone receptor (TSHR) and thyroid-stimulating immunoglobulin (TSI). We used a recently introduced, technically enhanced TSI bioassay to assess its diagnostic value and determine the cut-off in patients in high iodine intake area. METHODS: In a cross-sectional setting, we collected serum from 67 patients with untreated GD, 130 with GD under treatment, 22 with GD in remission, 42 with Hashimoto's thyroiditis, 12 with subacute thyroiditis, 20 with postpartum thyroiditis, and 93 euthyroid controls. TSI was measured using the Thyretain™ bioassay, which is based on Chinese hamster ovary cells transfected with chimeric TSHR (Mc4). TSI levels are reported as a specimen-to-reference ratio percentage (SRR%). RESULTS: The TSI levels in patients with GD (either treated or not) were significantly higher than those of the remaining patients (p < 0.05). The new bioassay showed a sensitivity of 97.0% and a specificity of 95.9% with a cut-off value of 123.0 SRR% for GD. A weak correlation was found between TSI and thyrotropin-binding inhibiting immunoglobulin (TBII) (r(s) = 0.259, p = 0.03), but no correlation was found between TSI and tri-iodothyronine or free thyroxine. CONCLUSIONS: The Mc4-CHO bioassay showed comparable diagnostic value for GD with the conventional TBII assay. We propose a cut-off of 123.0 SRR% in areas where iodine intake is high.
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spelling pubmed-31108502011-06-28 Diagnostic Value of a Chimeric TSH Receptor (Mc4)-Based Bioassay for Graves' Disease Lee, Ji In Jang, Hye Won Kim, Soo Kyoung Choi, Joon Young Kim, Ji Young Hur, Kyu Yeon Kim, Jae Hyeon Min, Yong-Ki Chung, Jae Hoon Kim, Sun Wook Korean J Intern Med Original Article BACKGROUND/AIMS: Graves' disease (GD) is caused by thyroid-stimulating hormone receptor (TSHR) and thyroid-stimulating immunoglobulin (TSI). We used a recently introduced, technically enhanced TSI bioassay to assess its diagnostic value and determine the cut-off in patients in high iodine intake area. METHODS: In a cross-sectional setting, we collected serum from 67 patients with untreated GD, 130 with GD under treatment, 22 with GD in remission, 42 with Hashimoto's thyroiditis, 12 with subacute thyroiditis, 20 with postpartum thyroiditis, and 93 euthyroid controls. TSI was measured using the Thyretain™ bioassay, which is based on Chinese hamster ovary cells transfected with chimeric TSHR (Mc4). TSI levels are reported as a specimen-to-reference ratio percentage (SRR%). RESULTS: The TSI levels in patients with GD (either treated or not) were significantly higher than those of the remaining patients (p < 0.05). The new bioassay showed a sensitivity of 97.0% and a specificity of 95.9% with a cut-off value of 123.0 SRR% for GD. A weak correlation was found between TSI and thyrotropin-binding inhibiting immunoglobulin (TBII) (r(s) = 0.259, p = 0.03), but no correlation was found between TSI and tri-iodothyronine or free thyroxine. CONCLUSIONS: The Mc4-CHO bioassay showed comparable diagnostic value for GD with the conventional TBII assay. We propose a cut-off of 123.0 SRR% in areas where iodine intake is high. The Korean Association of Internal Medicine 2011-06 2011-06-01 /pmc/articles/PMC3110850/ /pubmed/21716594 http://dx.doi.org/10.3904/kjim.2011.26.2.179 Text en Copyright © 2011 The Korean Association of Internal Medicine https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Lee, Ji In
Jang, Hye Won
Kim, Soo Kyoung
Choi, Joon Young
Kim, Ji Young
Hur, Kyu Yeon
Kim, Jae Hyeon
Min, Yong-Ki
Chung, Jae Hoon
Kim, Sun Wook
Diagnostic Value of a Chimeric TSH Receptor (Mc4)-Based Bioassay for Graves' Disease
title Diagnostic Value of a Chimeric TSH Receptor (Mc4)-Based Bioassay for Graves' Disease
title_full Diagnostic Value of a Chimeric TSH Receptor (Mc4)-Based Bioassay for Graves' Disease
title_fullStr Diagnostic Value of a Chimeric TSH Receptor (Mc4)-Based Bioassay for Graves' Disease
title_full_unstemmed Diagnostic Value of a Chimeric TSH Receptor (Mc4)-Based Bioassay for Graves' Disease
title_short Diagnostic Value of a Chimeric TSH Receptor (Mc4)-Based Bioassay for Graves' Disease
title_sort diagnostic value of a chimeric tsh receptor (mc4)-based bioassay for graves' disease
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3110850/
https://www.ncbi.nlm.nih.gov/pubmed/21716594
http://dx.doi.org/10.3904/kjim.2011.26.2.179
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