Cargando…
Diagnostic Value of a Chimeric TSH Receptor (Mc4)-Based Bioassay for Graves' Disease
BACKGROUND/AIMS: Graves' disease (GD) is caused by thyroid-stimulating hormone receptor (TSHR) and thyroid-stimulating immunoglobulin (TSI). We used a recently introduced, technically enhanced TSI bioassay to assess its diagnostic value and determine the cut-off in patients in high iodine intak...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Association of Internal Medicine
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3110850/ https://www.ncbi.nlm.nih.gov/pubmed/21716594 http://dx.doi.org/10.3904/kjim.2011.26.2.179 |
_version_ | 1782205561205424128 |
---|---|
author | Lee, Ji In Jang, Hye Won Kim, Soo Kyoung Choi, Joon Young Kim, Ji Young Hur, Kyu Yeon Kim, Jae Hyeon Min, Yong-Ki Chung, Jae Hoon Kim, Sun Wook |
author_facet | Lee, Ji In Jang, Hye Won Kim, Soo Kyoung Choi, Joon Young Kim, Ji Young Hur, Kyu Yeon Kim, Jae Hyeon Min, Yong-Ki Chung, Jae Hoon Kim, Sun Wook |
author_sort | Lee, Ji In |
collection | PubMed |
description | BACKGROUND/AIMS: Graves' disease (GD) is caused by thyroid-stimulating hormone receptor (TSHR) and thyroid-stimulating immunoglobulin (TSI). We used a recently introduced, technically enhanced TSI bioassay to assess its diagnostic value and determine the cut-off in patients in high iodine intake area. METHODS: In a cross-sectional setting, we collected serum from 67 patients with untreated GD, 130 with GD under treatment, 22 with GD in remission, 42 with Hashimoto's thyroiditis, 12 with subacute thyroiditis, 20 with postpartum thyroiditis, and 93 euthyroid controls. TSI was measured using the Thyretain™ bioassay, which is based on Chinese hamster ovary cells transfected with chimeric TSHR (Mc4). TSI levels are reported as a specimen-to-reference ratio percentage (SRR%). RESULTS: The TSI levels in patients with GD (either treated or not) were significantly higher than those of the remaining patients (p < 0.05). The new bioassay showed a sensitivity of 97.0% and a specificity of 95.9% with a cut-off value of 123.0 SRR% for GD. A weak correlation was found between TSI and thyrotropin-binding inhibiting immunoglobulin (TBII) (r(s) = 0.259, p = 0.03), but no correlation was found between TSI and tri-iodothyronine or free thyroxine. CONCLUSIONS: The Mc4-CHO bioassay showed comparable diagnostic value for GD with the conventional TBII assay. We propose a cut-off of 123.0 SRR% in areas where iodine intake is high. |
format | Online Article Text |
id | pubmed-3110850 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | The Korean Association of Internal Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-31108502011-06-28 Diagnostic Value of a Chimeric TSH Receptor (Mc4)-Based Bioassay for Graves' Disease Lee, Ji In Jang, Hye Won Kim, Soo Kyoung Choi, Joon Young Kim, Ji Young Hur, Kyu Yeon Kim, Jae Hyeon Min, Yong-Ki Chung, Jae Hoon Kim, Sun Wook Korean J Intern Med Original Article BACKGROUND/AIMS: Graves' disease (GD) is caused by thyroid-stimulating hormone receptor (TSHR) and thyroid-stimulating immunoglobulin (TSI). We used a recently introduced, technically enhanced TSI bioassay to assess its diagnostic value and determine the cut-off in patients in high iodine intake area. METHODS: In a cross-sectional setting, we collected serum from 67 patients with untreated GD, 130 with GD under treatment, 22 with GD in remission, 42 with Hashimoto's thyroiditis, 12 with subacute thyroiditis, 20 with postpartum thyroiditis, and 93 euthyroid controls. TSI was measured using the Thyretain™ bioassay, which is based on Chinese hamster ovary cells transfected with chimeric TSHR (Mc4). TSI levels are reported as a specimen-to-reference ratio percentage (SRR%). RESULTS: The TSI levels in patients with GD (either treated or not) were significantly higher than those of the remaining patients (p < 0.05). The new bioassay showed a sensitivity of 97.0% and a specificity of 95.9% with a cut-off value of 123.0 SRR% for GD. A weak correlation was found between TSI and thyrotropin-binding inhibiting immunoglobulin (TBII) (r(s) = 0.259, p = 0.03), but no correlation was found between TSI and tri-iodothyronine or free thyroxine. CONCLUSIONS: The Mc4-CHO bioassay showed comparable diagnostic value for GD with the conventional TBII assay. We propose a cut-off of 123.0 SRR% in areas where iodine intake is high. The Korean Association of Internal Medicine 2011-06 2011-06-01 /pmc/articles/PMC3110850/ /pubmed/21716594 http://dx.doi.org/10.3904/kjim.2011.26.2.179 Text en Copyright © 2011 The Korean Association of Internal Medicine https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Lee, Ji In Jang, Hye Won Kim, Soo Kyoung Choi, Joon Young Kim, Ji Young Hur, Kyu Yeon Kim, Jae Hyeon Min, Yong-Ki Chung, Jae Hoon Kim, Sun Wook Diagnostic Value of a Chimeric TSH Receptor (Mc4)-Based Bioassay for Graves' Disease |
title | Diagnostic Value of a Chimeric TSH Receptor (Mc4)-Based Bioassay for Graves' Disease |
title_full | Diagnostic Value of a Chimeric TSH Receptor (Mc4)-Based Bioassay for Graves' Disease |
title_fullStr | Diagnostic Value of a Chimeric TSH Receptor (Mc4)-Based Bioassay for Graves' Disease |
title_full_unstemmed | Diagnostic Value of a Chimeric TSH Receptor (Mc4)-Based Bioassay for Graves' Disease |
title_short | Diagnostic Value of a Chimeric TSH Receptor (Mc4)-Based Bioassay for Graves' Disease |
title_sort | diagnostic value of a chimeric tsh receptor (mc4)-based bioassay for graves' disease |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3110850/ https://www.ncbi.nlm.nih.gov/pubmed/21716594 http://dx.doi.org/10.3904/kjim.2011.26.2.179 |
work_keys_str_mv | AT leejiin diagnosticvalueofachimerictshreceptormc4basedbioassayforgravesdisease AT janghyewon diagnosticvalueofachimerictshreceptormc4basedbioassayforgravesdisease AT kimsookyoung diagnosticvalueofachimerictshreceptormc4basedbioassayforgravesdisease AT choijoonyoung diagnosticvalueofachimerictshreceptormc4basedbioassayforgravesdisease AT kimjiyoung diagnosticvalueofachimerictshreceptormc4basedbioassayforgravesdisease AT hurkyuyeon diagnosticvalueofachimerictshreceptormc4basedbioassayforgravesdisease AT kimjaehyeon diagnosticvalueofachimerictshreceptormc4basedbioassayforgravesdisease AT minyongki diagnosticvalueofachimerictshreceptormc4basedbioassayforgravesdisease AT chungjaehoon diagnosticvalueofachimerictshreceptormc4basedbioassayforgravesdisease AT kimsunwook diagnosticvalueofachimerictshreceptormc4basedbioassayforgravesdisease |