Cargando…
High throughput RNAi assay optimization using adherent cell cytometry
BACKGROUND: siRNA technology is a promising tool for gene therapy of vascular disease. Due to the multitude of reagents and cell types, RNAi experiment optimization can be time-consuming. In this study adherent cell cytometry was used to rapidly optimize siRNA transfection in human aortic vascular s...
Autores principales: | Nabzdyk, Christoph S, Chun, Maggie, Pradhan, Leena, LoGerfo, Frank W |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3111359/ https://www.ncbi.nlm.nih.gov/pubmed/21518450 http://dx.doi.org/10.1186/1479-5876-9-48 |
Ejemplares similares
-
RNAi therapy to the wall of arteries and veins: anatomical, physiologic, and pharmacological considerations
por: Nabzdyk, Christoph S., et al.
Publicado: (2017) -
Temporal Network Based Analysis of Cell Specific Vein Graft Transcriptome Defines Key Pathways and Hub Genes in Implantation Injury
por: Bhasin, Manoj, et al.
Publicado: (2012) -
The effects of transfection reagent polyethyleneimine (PEI) and non-targeting control siRNAs on global gene expression in human aortic smooth muscle cells
por: Raof, Nurazhani A., et al.
Publicado: (2016) -
Development of a high-throughput γ-H2AX assay based on imaging flow cytometry
por: Lee, Younghyun, et al.
Publicado: (2019) -
Optimizing transformations for automated, high throughput analysis of flow cytometry data
por: Finak, Greg, et al.
Publicado: (2010)