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Genetic analyses place most Spanish isolates of Beauveria bassiana in a molecular group with word-wide distribution

BACKGROUND: The entomopathogenic anamorphic fungus Beauveria bassiana is currently used as a biocontrol agent (BCA) of insects. Fifty-seven Beauveria bassiana isolates -53 from Spain- were characterized, integrating group I intron insertion patterns at the 3'-end of the nuclear large subunit ri...

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Autores principales: Garrido-Jurado, Inmaculada, Márquez, Marcela, Ortiz-Urquiza, Almudena, Santiago-Álvarez, Cándido, Iturriaga, Enrique A, Quesada-Moraga, Enrique, Monte, Enrique, Hermosa, Rosa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3111375/
https://www.ncbi.nlm.nih.gov/pubmed/21521527
http://dx.doi.org/10.1186/1471-2180-11-84
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author Garrido-Jurado, Inmaculada
Márquez, Marcela
Ortiz-Urquiza, Almudena
Santiago-Álvarez, Cándido
Iturriaga, Enrique A
Quesada-Moraga, Enrique
Monte, Enrique
Hermosa, Rosa
author_facet Garrido-Jurado, Inmaculada
Márquez, Marcela
Ortiz-Urquiza, Almudena
Santiago-Álvarez, Cándido
Iturriaga, Enrique A
Quesada-Moraga, Enrique
Monte, Enrique
Hermosa, Rosa
author_sort Garrido-Jurado, Inmaculada
collection PubMed
description BACKGROUND: The entomopathogenic anamorphic fungus Beauveria bassiana is currently used as a biocontrol agent (BCA) of insects. Fifty-seven Beauveria bassiana isolates -53 from Spain- were characterized, integrating group I intron insertion patterns at the 3'-end of the nuclear large subunit ribosomal gene (LSU rDNA) and elongation factor 1-alpha (EF1-α) phylogenetic information, in order to assess the genetic structure and diversity of this Spanish collection of B. bassiana. RESULTS: Group I intron genotype analysis was based on the four highly conserved insertion sites of the LSU (Ec2653, Ec2449, Ec2066, Ec1921). Of the 16 possible combinations/genotypes, only four were detected, two of which were predominant, containing 44 and 9 members out of 57 isolates, respectively. Interestingly, the members of the latter two genotypes showed unique differences in their growth temperatures. In follow, EF1-α phylogeny served to classify most of the strains in the B. bassiana s.s. (sensu stricto) group and separate them into 5 molecular subgroups, all of which contained a group I intron belonging to the IC1 subtype at the Ec1921 position. A number of parameters such as thermal growth or origin (host, geographic location and climatic conditions) were also examined but in general no association could be found. CONCLUSION: Most Spanish B. bassiana isolates (77.2%) are grouped into a major phylogenetic subgroup with word-wide distribution. However, high phylogenetic diversity was also detected among Spanish isolates from close geographic zones with low climatic variation. In general, no correlation was observed between the molecular distribution and geographic origin or climatic characteristics where the Spanish B. bassiana isolates were sampled.
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spelling pubmed-31113752011-06-10 Genetic analyses place most Spanish isolates of Beauveria bassiana in a molecular group with word-wide distribution Garrido-Jurado, Inmaculada Márquez, Marcela Ortiz-Urquiza, Almudena Santiago-Álvarez, Cándido Iturriaga, Enrique A Quesada-Moraga, Enrique Monte, Enrique Hermosa, Rosa BMC Microbiol Research Article BACKGROUND: The entomopathogenic anamorphic fungus Beauveria bassiana is currently used as a biocontrol agent (BCA) of insects. Fifty-seven Beauveria bassiana isolates -53 from Spain- were characterized, integrating group I intron insertion patterns at the 3'-end of the nuclear large subunit ribosomal gene (LSU rDNA) and elongation factor 1-alpha (EF1-α) phylogenetic information, in order to assess the genetic structure and diversity of this Spanish collection of B. bassiana. RESULTS: Group I intron genotype analysis was based on the four highly conserved insertion sites of the LSU (Ec2653, Ec2449, Ec2066, Ec1921). Of the 16 possible combinations/genotypes, only four were detected, two of which were predominant, containing 44 and 9 members out of 57 isolates, respectively. Interestingly, the members of the latter two genotypes showed unique differences in their growth temperatures. In follow, EF1-α phylogeny served to classify most of the strains in the B. bassiana s.s. (sensu stricto) group and separate them into 5 molecular subgroups, all of which contained a group I intron belonging to the IC1 subtype at the Ec1921 position. A number of parameters such as thermal growth or origin (host, geographic location and climatic conditions) were also examined but in general no association could be found. CONCLUSION: Most Spanish B. bassiana isolates (77.2%) are grouped into a major phylogenetic subgroup with word-wide distribution. However, high phylogenetic diversity was also detected among Spanish isolates from close geographic zones with low climatic variation. In general, no correlation was observed between the molecular distribution and geographic origin or climatic characteristics where the Spanish B. bassiana isolates were sampled. BioMed Central 2011-04-26 /pmc/articles/PMC3111375/ /pubmed/21521527 http://dx.doi.org/10.1186/1471-2180-11-84 Text en Copyright ©2011 Garrido-Jurado et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Garrido-Jurado, Inmaculada
Márquez, Marcela
Ortiz-Urquiza, Almudena
Santiago-Álvarez, Cándido
Iturriaga, Enrique A
Quesada-Moraga, Enrique
Monte, Enrique
Hermosa, Rosa
Genetic analyses place most Spanish isolates of Beauveria bassiana in a molecular group with word-wide distribution
title Genetic analyses place most Spanish isolates of Beauveria bassiana in a molecular group with word-wide distribution
title_full Genetic analyses place most Spanish isolates of Beauveria bassiana in a molecular group with word-wide distribution
title_fullStr Genetic analyses place most Spanish isolates of Beauveria bassiana in a molecular group with word-wide distribution
title_full_unstemmed Genetic analyses place most Spanish isolates of Beauveria bassiana in a molecular group with word-wide distribution
title_short Genetic analyses place most Spanish isolates of Beauveria bassiana in a molecular group with word-wide distribution
title_sort genetic analyses place most spanish isolates of beauveria bassiana in a molecular group with word-wide distribution
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3111375/
https://www.ncbi.nlm.nih.gov/pubmed/21521527
http://dx.doi.org/10.1186/1471-2180-11-84
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