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Genome-wide linkage study of atopic dermatitis in West Highland White Terriers
BACKGROUND: Canine atopic dermatitis (AD) is a common, heritable, chronic allergic skin condition prevalent in the West Highland White Terrier (WHWT). In canine AD, environmental allergens trigger an inflammatory response causing visible skin lesions and chronic pruritus that can lead to secondary b...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3111396/ https://www.ncbi.nlm.nih.gov/pubmed/21510878 http://dx.doi.org/10.1186/1471-2156-12-37 |
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author | Salzmann, Cary A Olivry, Thierry JM Nielsen, Dahlia M Paps, Judith S Harris, Tonya L Olby, Natasha J |
author_facet | Salzmann, Cary A Olivry, Thierry JM Nielsen, Dahlia M Paps, Judith S Harris, Tonya L Olby, Natasha J |
author_sort | Salzmann, Cary A |
collection | PubMed |
description | BACKGROUND: Canine atopic dermatitis (AD) is a common, heritable, chronic allergic skin condition prevalent in the West Highland White Terrier (WHWT). In canine AD, environmental allergens trigger an inflammatory response causing visible skin lesions and chronic pruritus that can lead to secondary bacterial and yeast infections. The disorder shares many of the clinical and histopathological characteristics of human AD and represents an animal model of this disorder that could be used to further elucidate genetic causes of human AD. Microsatellite markers genotyped in families of WHWTs affected with AD were used to perform a genome-wide linkage study in order to isolate chromosomal regions associated with the disorder. RESULTS: Blood samples and health questionnaires were collected from 108 WHWTs spanning three families. A linkage simulation using these 108 dogs showed high power to detect a highly penetrant mutation. Ninety WHWTs were genotyped using markers from the Minimal Screening Set 2 (MSS-2). Two hundred and fifty six markers were informative and were used for linkage analysis. Using a LOD score of 2.7 as a significance threshold, no chromosomal regions were identified with significant linkage to AD. LOD scores greater than 1.0 were located in a 56 cM region of chromosome 7. CONCLUSIONS: The study was unable to detect any chromosomal regions significantly linked to canine AD. This could be a result of factors such as environmental modification of phenotype, incorrect assignment of phenotype, a mutation of low penetrance, or incomplete genome coverage. A genome-wide SNP association study in a larger cohort of WHWTs may prove more successful by providing higher density coverage and higher statistical power. |
format | Online Article Text |
id | pubmed-3111396 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-31113962011-06-10 Genome-wide linkage study of atopic dermatitis in West Highland White Terriers Salzmann, Cary A Olivry, Thierry JM Nielsen, Dahlia M Paps, Judith S Harris, Tonya L Olby, Natasha J BMC Genet Research Article BACKGROUND: Canine atopic dermatitis (AD) is a common, heritable, chronic allergic skin condition prevalent in the West Highland White Terrier (WHWT). In canine AD, environmental allergens trigger an inflammatory response causing visible skin lesions and chronic pruritus that can lead to secondary bacterial and yeast infections. The disorder shares many of the clinical and histopathological characteristics of human AD and represents an animal model of this disorder that could be used to further elucidate genetic causes of human AD. Microsatellite markers genotyped in families of WHWTs affected with AD were used to perform a genome-wide linkage study in order to isolate chromosomal regions associated with the disorder. RESULTS: Blood samples and health questionnaires were collected from 108 WHWTs spanning three families. A linkage simulation using these 108 dogs showed high power to detect a highly penetrant mutation. Ninety WHWTs were genotyped using markers from the Minimal Screening Set 2 (MSS-2). Two hundred and fifty six markers were informative and were used for linkage analysis. Using a LOD score of 2.7 as a significance threshold, no chromosomal regions were identified with significant linkage to AD. LOD scores greater than 1.0 were located in a 56 cM region of chromosome 7. CONCLUSIONS: The study was unable to detect any chromosomal regions significantly linked to canine AD. This could be a result of factors such as environmental modification of phenotype, incorrect assignment of phenotype, a mutation of low penetrance, or incomplete genome coverage. A genome-wide SNP association study in a larger cohort of WHWTs may prove more successful by providing higher density coverage and higher statistical power. BioMed Central 2011-04-21 /pmc/articles/PMC3111396/ /pubmed/21510878 http://dx.doi.org/10.1186/1471-2156-12-37 Text en Copyright ©2011 Salzmann et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Salzmann, Cary A Olivry, Thierry JM Nielsen, Dahlia M Paps, Judith S Harris, Tonya L Olby, Natasha J Genome-wide linkage study of atopic dermatitis in West Highland White Terriers |
title | Genome-wide linkage study of atopic dermatitis in West Highland White Terriers |
title_full | Genome-wide linkage study of atopic dermatitis in West Highland White Terriers |
title_fullStr | Genome-wide linkage study of atopic dermatitis in West Highland White Terriers |
title_full_unstemmed | Genome-wide linkage study of atopic dermatitis in West Highland White Terriers |
title_short | Genome-wide linkage study of atopic dermatitis in West Highland White Terriers |
title_sort | genome-wide linkage study of atopic dermatitis in west highland white terriers |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3111396/ https://www.ncbi.nlm.nih.gov/pubmed/21510878 http://dx.doi.org/10.1186/1471-2156-12-37 |
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