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Molecular Interactions that Enable Movement of the Lyme Disease Agent from the Tick Gut into the Hemolymph

Borrelia burgdorferi, the causative agent of Lyme disease, is transmitted to humans by bite of Ixodes scapularis ticks. The mechanisms by which the bacterium is transmitted from vector to host are poorly understood. In this study, we show that the F(ab)(2) fragments of BBE31, a B.burgdorferi outer-s...

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Autores principales: Zhang, Lili, Zhang, Yue, Adusumilli, Sarojini, Liu, Lei, Narasimhan, Sukanya, Dai, Jianfeng, Zhao, Yang O., Fikrig, Erol
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3111543/
https://www.ncbi.nlm.nih.gov/pubmed/21695244
http://dx.doi.org/10.1371/journal.ppat.1002079
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author Zhang, Lili
Zhang, Yue
Adusumilli, Sarojini
Liu, Lei
Narasimhan, Sukanya
Dai, Jianfeng
Zhao, Yang O.
Fikrig, Erol
author_facet Zhang, Lili
Zhang, Yue
Adusumilli, Sarojini
Liu, Lei
Narasimhan, Sukanya
Dai, Jianfeng
Zhao, Yang O.
Fikrig, Erol
author_sort Zhang, Lili
collection PubMed
description Borrelia burgdorferi, the causative agent of Lyme disease, is transmitted to humans by bite of Ixodes scapularis ticks. The mechanisms by which the bacterium is transmitted from vector to host are poorly understood. In this study, we show that the F(ab)(2) fragments of BBE31, a B.burgdorferi outer-surface lipoprotein, interfere with the migration of the spirochete from tick gut into the hemolymph during tick feeding. The decreased hemolymph infection results in lower salivary glands infection, and consequently attenuates mouse infection by tick-transmitted B. burgdorferi. Using a yeast surface display approach, a tick gut protein named TRE31 was identified to interact with BBE31. Silencing tre31 also decreased the B. burgdorferi burden in the tick hemolymph. Delineating the specific spirochete and arthropod ligands required for B. burgdorferi movement in the tick may lead to new strategies to interrupt the life cycle of the Lyme disease agent.
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spelling pubmed-31115432011-06-21 Molecular Interactions that Enable Movement of the Lyme Disease Agent from the Tick Gut into the Hemolymph Zhang, Lili Zhang, Yue Adusumilli, Sarojini Liu, Lei Narasimhan, Sukanya Dai, Jianfeng Zhao, Yang O. Fikrig, Erol PLoS Pathog Research Article Borrelia burgdorferi, the causative agent of Lyme disease, is transmitted to humans by bite of Ixodes scapularis ticks. The mechanisms by which the bacterium is transmitted from vector to host are poorly understood. In this study, we show that the F(ab)(2) fragments of BBE31, a B.burgdorferi outer-surface lipoprotein, interfere with the migration of the spirochete from tick gut into the hemolymph during tick feeding. The decreased hemolymph infection results in lower salivary glands infection, and consequently attenuates mouse infection by tick-transmitted B. burgdorferi. Using a yeast surface display approach, a tick gut protein named TRE31 was identified to interact with BBE31. Silencing tre31 also decreased the B. burgdorferi burden in the tick hemolymph. Delineating the specific spirochete and arthropod ligands required for B. burgdorferi movement in the tick may lead to new strategies to interrupt the life cycle of the Lyme disease agent. Public Library of Science 2011-06-09 /pmc/articles/PMC3111543/ /pubmed/21695244 http://dx.doi.org/10.1371/journal.ppat.1002079 Text en Zhang et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zhang, Lili
Zhang, Yue
Adusumilli, Sarojini
Liu, Lei
Narasimhan, Sukanya
Dai, Jianfeng
Zhao, Yang O.
Fikrig, Erol
Molecular Interactions that Enable Movement of the Lyme Disease Agent from the Tick Gut into the Hemolymph
title Molecular Interactions that Enable Movement of the Lyme Disease Agent from the Tick Gut into the Hemolymph
title_full Molecular Interactions that Enable Movement of the Lyme Disease Agent from the Tick Gut into the Hemolymph
title_fullStr Molecular Interactions that Enable Movement of the Lyme Disease Agent from the Tick Gut into the Hemolymph
title_full_unstemmed Molecular Interactions that Enable Movement of the Lyme Disease Agent from the Tick Gut into the Hemolymph
title_short Molecular Interactions that Enable Movement of the Lyme Disease Agent from the Tick Gut into the Hemolymph
title_sort molecular interactions that enable movement of the lyme disease agent from the tick gut into the hemolymph
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3111543/
https://www.ncbi.nlm.nih.gov/pubmed/21695244
http://dx.doi.org/10.1371/journal.ppat.1002079
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