Global gene disruption in human cells to assign genes to phenotypes

Insertional mutagenesis in a haploid background can lead to complete disruption of gene function(1). Here we generate a population of human cells that contain insertions in >98% of their expressed genes. We established Phenotypic Interrogation via Tag Sequencing (PhITSeq) as a method to examine m...

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Detalles Bibliográficos
Autores principales: Carette, Jan E., Guimaraes, Carla P., Wuethrich, Irene, Blomen, Vincent A., Varadarajan, Malini, Sun, Chong, Bell, George, Yuan, Bingbing, Muellner, Markus K., Nijman, Sebastian M., Ploegh, Hidde L., Brummelkamp, Thijn R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3111863/
https://www.ncbi.nlm.nih.gov/pubmed/21623355
http://dx.doi.org/10.1038/nbt.1857

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3111863/
https://www.ncbi.nlm.nih.gov/pubmed/21623355
http://dx.doi.org/10.1038/nbt.1857

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