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Global gene disruption in human cells to assign genes to phenotypes
Insertional mutagenesis in a haploid background can lead to complete disruption of gene function(1). Here we generate a population of human cells that contain insertions in >98% of their expressed genes. We established Phenotypic Interrogation via Tag Sequencing (PhITSeq) as a method to examine m...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2011
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3111863/ https://www.ncbi.nlm.nih.gov/pubmed/21623355 http://dx.doi.org/10.1038/nbt.1857 |