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Genome-wide chromatin occupancy analysis reveals a role for ASH2 in transcriptional pausing
An important mechanism for gene regulation involves chromatin changes via histone modification. One such modification is histone H3 lysine 4 trimethylation (H3K4me3), which requires histone methyltranferase complexes (HMT) containing the trithorax-group (trxG) protein ASH2. Mutations in ash2 cause a...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3113561/ https://www.ncbi.nlm.nih.gov/pubmed/21310711 http://dx.doi.org/10.1093/nar/gkq1322 |
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author | Pérez-Lluch, Sílvia Blanco, Enrique Carbonell, Albert Raha, Debasish Snyder, Michael Serras, Florenci Corominas, Montserrat |
author_facet | Pérez-Lluch, Sílvia Blanco, Enrique Carbonell, Albert Raha, Debasish Snyder, Michael Serras, Florenci Corominas, Montserrat |
author_sort | Pérez-Lluch, Sílvia |
collection | PubMed |
description | An important mechanism for gene regulation involves chromatin changes via histone modification. One such modification is histone H3 lysine 4 trimethylation (H3K4me3), which requires histone methyltranferase complexes (HMT) containing the trithorax-group (trxG) protein ASH2. Mutations in ash2 cause a variety of pattern formation defects in the Drosophila wing. We have identified genome-wide binding of ASH2 in wing imaginal discs using chromatin immunoprecipitation combined with sequencing (ChIP-Seq). Our results show that genes with functions in development and transcriptional regulation are activated by ASH2 via H3K4 trimethylation in nearby nucleosomes. We have characterized the occupancy of phosphorylated forms of RNA Polymerase II and histone marks associated with activation and repression of transcription. ASH2 occupancy correlates with phosphorylated forms of RNA Polymerase II and histone activating marks in expressed genes. Additionally, RNA Polymerase II phosphorylation on serine 5 and H3K4me3 are reduced in ash2 mutants in comparison to wild-type flies. Finally, we have identified specific motifs associated with ASH2 binding in genes that are differentially expressed in ash2 mutants. Our data suggest that recruitment of the ASH2-containing HMT complexes is context specific and points to a function of ASH2 and H3K4me3 in transcriptional pausing control. |
format | Online Article Text |
id | pubmed-3113561 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-31135612011-06-14 Genome-wide chromatin occupancy analysis reveals a role for ASH2 in transcriptional pausing Pérez-Lluch, Sílvia Blanco, Enrique Carbonell, Albert Raha, Debasish Snyder, Michael Serras, Florenci Corominas, Montserrat Nucleic Acids Res Gene Regulation, Chromatin and Epigenetics An important mechanism for gene regulation involves chromatin changes via histone modification. One such modification is histone H3 lysine 4 trimethylation (H3K4me3), which requires histone methyltranferase complexes (HMT) containing the trithorax-group (trxG) protein ASH2. Mutations in ash2 cause a variety of pattern formation defects in the Drosophila wing. We have identified genome-wide binding of ASH2 in wing imaginal discs using chromatin immunoprecipitation combined with sequencing (ChIP-Seq). Our results show that genes with functions in development and transcriptional regulation are activated by ASH2 via H3K4 trimethylation in nearby nucleosomes. We have characterized the occupancy of phosphorylated forms of RNA Polymerase II and histone marks associated with activation and repression of transcription. ASH2 occupancy correlates with phosphorylated forms of RNA Polymerase II and histone activating marks in expressed genes. Additionally, RNA Polymerase II phosphorylation on serine 5 and H3K4me3 are reduced in ash2 mutants in comparison to wild-type flies. Finally, we have identified specific motifs associated with ASH2 binding in genes that are differentially expressed in ash2 mutants. Our data suggest that recruitment of the ASH2-containing HMT complexes is context specific and points to a function of ASH2 and H3K4me3 in transcriptional pausing control. Oxford University Press 2011-06 2011-02-09 /pmc/articles/PMC3113561/ /pubmed/21310711 http://dx.doi.org/10.1093/nar/gkq1322 Text en © The Author(s) 2011. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/2.5 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Gene Regulation, Chromatin and Epigenetics Pérez-Lluch, Sílvia Blanco, Enrique Carbonell, Albert Raha, Debasish Snyder, Michael Serras, Florenci Corominas, Montserrat Genome-wide chromatin occupancy analysis reveals a role for ASH2 in transcriptional pausing |
title | Genome-wide chromatin occupancy analysis reveals a role for ASH2 in transcriptional pausing |
title_full | Genome-wide chromatin occupancy analysis reveals a role for ASH2 in transcriptional pausing |
title_fullStr | Genome-wide chromatin occupancy analysis reveals a role for ASH2 in transcriptional pausing |
title_full_unstemmed | Genome-wide chromatin occupancy analysis reveals a role for ASH2 in transcriptional pausing |
title_short | Genome-wide chromatin occupancy analysis reveals a role for ASH2 in transcriptional pausing |
title_sort | genome-wide chromatin occupancy analysis reveals a role for ash2 in transcriptional pausing |
topic | Gene Regulation, Chromatin and Epigenetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3113561/ https://www.ncbi.nlm.nih.gov/pubmed/21310711 http://dx.doi.org/10.1093/nar/gkq1322 |
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