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Genome-wide chromatin occupancy analysis reveals a role for ASH2 in transcriptional pausing

An important mechanism for gene regulation involves chromatin changes via histone modification. One such modification is histone H3 lysine 4 trimethylation (H3K4me3), which requires histone methyltranferase complexes (HMT) containing the trithorax-group (trxG) protein ASH2. Mutations in ash2 cause a...

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Autores principales: Pérez-Lluch, Sílvia, Blanco, Enrique, Carbonell, Albert, Raha, Debasish, Snyder, Michael, Serras, Florenci, Corominas, Montserrat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3113561/
https://www.ncbi.nlm.nih.gov/pubmed/21310711
http://dx.doi.org/10.1093/nar/gkq1322
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author Pérez-Lluch, Sílvia
Blanco, Enrique
Carbonell, Albert
Raha, Debasish
Snyder, Michael
Serras, Florenci
Corominas, Montserrat
author_facet Pérez-Lluch, Sílvia
Blanco, Enrique
Carbonell, Albert
Raha, Debasish
Snyder, Michael
Serras, Florenci
Corominas, Montserrat
author_sort Pérez-Lluch, Sílvia
collection PubMed
description An important mechanism for gene regulation involves chromatin changes via histone modification. One such modification is histone H3 lysine 4 trimethylation (H3K4me3), which requires histone methyltranferase complexes (HMT) containing the trithorax-group (trxG) protein ASH2. Mutations in ash2 cause a variety of pattern formation defects in the Drosophila wing. We have identified genome-wide binding of ASH2 in wing imaginal discs using chromatin immunoprecipitation combined with sequencing (ChIP-Seq). Our results show that genes with functions in development and transcriptional regulation are activated by ASH2 via H3K4 trimethylation in nearby nucleosomes. We have characterized the occupancy of phosphorylated forms of RNA Polymerase II and histone marks associated with activation and repression of transcription. ASH2 occupancy correlates with phosphorylated forms of RNA Polymerase II and histone activating marks in expressed genes. Additionally, RNA Polymerase II phosphorylation on serine 5 and H3K4me3 are reduced in ash2 mutants in comparison to wild-type flies. Finally, we have identified specific motifs associated with ASH2 binding in genes that are differentially expressed in ash2 mutants. Our data suggest that recruitment of the ASH2-containing HMT complexes is context specific and points to a function of ASH2 and H3K4me3 in transcriptional pausing control.
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spelling pubmed-31135612011-06-14 Genome-wide chromatin occupancy analysis reveals a role for ASH2 in transcriptional pausing Pérez-Lluch, Sílvia Blanco, Enrique Carbonell, Albert Raha, Debasish Snyder, Michael Serras, Florenci Corominas, Montserrat Nucleic Acids Res Gene Regulation, Chromatin and Epigenetics An important mechanism for gene regulation involves chromatin changes via histone modification. One such modification is histone H3 lysine 4 trimethylation (H3K4me3), which requires histone methyltranferase complexes (HMT) containing the trithorax-group (trxG) protein ASH2. Mutations in ash2 cause a variety of pattern formation defects in the Drosophila wing. We have identified genome-wide binding of ASH2 in wing imaginal discs using chromatin immunoprecipitation combined with sequencing (ChIP-Seq). Our results show that genes with functions in development and transcriptional regulation are activated by ASH2 via H3K4 trimethylation in nearby nucleosomes. We have characterized the occupancy of phosphorylated forms of RNA Polymerase II and histone marks associated with activation and repression of transcription. ASH2 occupancy correlates with phosphorylated forms of RNA Polymerase II and histone activating marks in expressed genes. Additionally, RNA Polymerase II phosphorylation on serine 5 and H3K4me3 are reduced in ash2 mutants in comparison to wild-type flies. Finally, we have identified specific motifs associated with ASH2 binding in genes that are differentially expressed in ash2 mutants. Our data suggest that recruitment of the ASH2-containing HMT complexes is context specific and points to a function of ASH2 and H3K4me3 in transcriptional pausing control. Oxford University Press 2011-06 2011-02-09 /pmc/articles/PMC3113561/ /pubmed/21310711 http://dx.doi.org/10.1093/nar/gkq1322 Text en © The Author(s) 2011. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/2.5 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Gene Regulation, Chromatin and Epigenetics
Pérez-Lluch, Sílvia
Blanco, Enrique
Carbonell, Albert
Raha, Debasish
Snyder, Michael
Serras, Florenci
Corominas, Montserrat
Genome-wide chromatin occupancy analysis reveals a role for ASH2 in transcriptional pausing
title Genome-wide chromatin occupancy analysis reveals a role for ASH2 in transcriptional pausing
title_full Genome-wide chromatin occupancy analysis reveals a role for ASH2 in transcriptional pausing
title_fullStr Genome-wide chromatin occupancy analysis reveals a role for ASH2 in transcriptional pausing
title_full_unstemmed Genome-wide chromatin occupancy analysis reveals a role for ASH2 in transcriptional pausing
title_short Genome-wide chromatin occupancy analysis reveals a role for ASH2 in transcriptional pausing
title_sort genome-wide chromatin occupancy analysis reveals a role for ash2 in transcriptional pausing
topic Gene Regulation, Chromatin and Epigenetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3113561/
https://www.ncbi.nlm.nih.gov/pubmed/21310711
http://dx.doi.org/10.1093/nar/gkq1322
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