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Frictional Behavior of Individual Vascular Smooth Muscle Cells Assessed By Lateral Force Microscopy

With the advancement of the field of biotribology, considerable interest has arisen in the study of cell and tissue frictional properties. From the perspective of medical device development, the frictional properties between a rigid surface and underlying cells and tissues are of a particular clinic...

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Autores principales: Dean, Delphine, Hemmer, Jason, Vertegel, Alexey, LaBerge, Martine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3113676/
https://www.ncbi.nlm.nih.gov/pubmed/21686041
http://dx.doi.org/10.3390/ma3094668
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author Dean, Delphine
Hemmer, Jason
Vertegel, Alexey
LaBerge, Martine
author_facet Dean, Delphine
Hemmer, Jason
Vertegel, Alexey
LaBerge, Martine
author_sort Dean, Delphine
collection PubMed
description With the advancement of the field of biotribology, considerable interest has arisen in the study of cell and tissue frictional properties. From the perspective of medical device development, the frictional properties between a rigid surface and underlying cells and tissues are of a particular clinical interest. As with many bearing surfaces, it is likely that contact asperities exist at the size scale of single cells and below. Thus, a technique to measure cellular frictional properties directly would be beneficial from both a clinical and a basic science perspective. In the current study, an atomic force microscope (AFM) with a 5 µm diameter borosilicate spherical probe simulating endovascular metallic stent asperities was used to characterize the surface frictional properties of vascular smooth muscle cells (VSMCs) in contact with a metallic endovascular stent. Various treatments were used to alter cell structure, in order to better understand the cellular components and mechanisms responsible for governing frictional properties. The frictional coefficient of the probe on VSMCs was found to be approximately 0.06. This frictional coefficient was significantly affected by cellular crosslinking and cytoskeletal depolymerization agents. These results demonstrate that AFM-based lateral force microscopy is a valuable technique to assess the friction properties of individual single cells on the micro-scale.
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spelling pubmed-31136762011-06-13 Frictional Behavior of Individual Vascular Smooth Muscle Cells Assessed By Lateral Force Microscopy Dean, Delphine Hemmer, Jason Vertegel, Alexey LaBerge, Martine Materials (Basel) Article With the advancement of the field of biotribology, considerable interest has arisen in the study of cell and tissue frictional properties. From the perspective of medical device development, the frictional properties between a rigid surface and underlying cells and tissues are of a particular clinical interest. As with many bearing surfaces, it is likely that contact asperities exist at the size scale of single cells and below. Thus, a technique to measure cellular frictional properties directly would be beneficial from both a clinical and a basic science perspective. In the current study, an atomic force microscope (AFM) with a 5 µm diameter borosilicate spherical probe simulating endovascular metallic stent asperities was used to characterize the surface frictional properties of vascular smooth muscle cells (VSMCs) in contact with a metallic endovascular stent. Various treatments were used to alter cell structure, in order to better understand the cellular components and mechanisms responsible for governing frictional properties. The frictional coefficient of the probe on VSMCs was found to be approximately 0.06. This frictional coefficient was significantly affected by cellular crosslinking and cytoskeletal depolymerization agents. These results demonstrate that AFM-based lateral force microscopy is a valuable technique to assess the friction properties of individual single cells on the micro-scale. MDPI 2010-09-14 /pmc/articles/PMC3113676/ /pubmed/21686041 http://dx.doi.org/10.3390/ma3094668 Text en © 2010 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Dean, Delphine
Hemmer, Jason
Vertegel, Alexey
LaBerge, Martine
Frictional Behavior of Individual Vascular Smooth Muscle Cells Assessed By Lateral Force Microscopy
title Frictional Behavior of Individual Vascular Smooth Muscle Cells Assessed By Lateral Force Microscopy
title_full Frictional Behavior of Individual Vascular Smooth Muscle Cells Assessed By Lateral Force Microscopy
title_fullStr Frictional Behavior of Individual Vascular Smooth Muscle Cells Assessed By Lateral Force Microscopy
title_full_unstemmed Frictional Behavior of Individual Vascular Smooth Muscle Cells Assessed By Lateral Force Microscopy
title_short Frictional Behavior of Individual Vascular Smooth Muscle Cells Assessed By Lateral Force Microscopy
title_sort frictional behavior of individual vascular smooth muscle cells assessed by lateral force microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3113676/
https://www.ncbi.nlm.nih.gov/pubmed/21686041
http://dx.doi.org/10.3390/ma3094668
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