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Coupling among growth rate response, metabolic cycle, and cell division cycle in yeast

We studied the steady-state responses to changes in growth rate of yeast when ethanol is the sole source of carbon and energy. Analysis of these data, together with data from studies where glucose was the carbon source, allowed us to distinguish a “universal” growth rate response (GRR) common to all...

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Autores principales: Slavov, Nikolai, Botstein, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3113766/
https://www.ncbi.nlm.nih.gov/pubmed/21525243
http://dx.doi.org/10.1091/mbc.E11-02-0132
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author Slavov, Nikolai
Botstein, David
author_facet Slavov, Nikolai
Botstein, David
author_sort Slavov, Nikolai
collection PubMed
description We studied the steady-state responses to changes in growth rate of yeast when ethanol is the sole source of carbon and energy. Analysis of these data, together with data from studies where glucose was the carbon source, allowed us to distinguish a “universal” growth rate response (GRR) common to all media studied from a GRR specific to the carbon source. Genes with positive universal GRR include ribosomal, translation, and mitochondrial genes, and those with negative GRR include autophagy, vacuolar, and stress response genes. The carbon source–specific GRR genes control mitochondrial function, peroxisomes, and synthesis of vitamins and cofactors, suggesting this response may reflect the intensity of oxidative metabolism. All genes with universal GRR, which comprise 25% of the genome, are expressed periodically in the yeast metabolic cycle (YMC). We propose that the universal GRR may be accounted for by changes in the relative durations of the YMC phases. This idea is supported by oxygen consumption data from metabolically synchronized cultures with doubling times ranging from 5 to 14 h. We found that the high oxygen consumption phase of the YMC can coincide exactly with the S phase of the cell division cycle, suggesting that oxidative metabolism and DNA replication are not incompatible.
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spelling pubmed-31137662011-08-30 Coupling among growth rate response, metabolic cycle, and cell division cycle in yeast Slavov, Nikolai Botstein, David Mol Biol Cell Articles We studied the steady-state responses to changes in growth rate of yeast when ethanol is the sole source of carbon and energy. Analysis of these data, together with data from studies where glucose was the carbon source, allowed us to distinguish a “universal” growth rate response (GRR) common to all media studied from a GRR specific to the carbon source. Genes with positive universal GRR include ribosomal, translation, and mitochondrial genes, and those with negative GRR include autophagy, vacuolar, and stress response genes. The carbon source–specific GRR genes control mitochondrial function, peroxisomes, and synthesis of vitamins and cofactors, suggesting this response may reflect the intensity of oxidative metabolism. All genes with universal GRR, which comprise 25% of the genome, are expressed periodically in the yeast metabolic cycle (YMC). We propose that the universal GRR may be accounted for by changes in the relative durations of the YMC phases. This idea is supported by oxygen consumption data from metabolically synchronized cultures with doubling times ranging from 5 to 14 h. We found that the high oxygen consumption phase of the YMC can coincide exactly with the S phase of the cell division cycle, suggesting that oxidative metabolism and DNA replication are not incompatible. The American Society for Cell Biology 2011-06-15 /pmc/articles/PMC3113766/ /pubmed/21525243 http://dx.doi.org/10.1091/mbc.E11-02-0132 Text en © 2011 Slavov and Botstein. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Slavov, Nikolai
Botstein, David
Coupling among growth rate response, metabolic cycle, and cell division cycle in yeast
title Coupling among growth rate response, metabolic cycle, and cell division cycle in yeast
title_full Coupling among growth rate response, metabolic cycle, and cell division cycle in yeast
title_fullStr Coupling among growth rate response, metabolic cycle, and cell division cycle in yeast
title_full_unstemmed Coupling among growth rate response, metabolic cycle, and cell division cycle in yeast
title_short Coupling among growth rate response, metabolic cycle, and cell division cycle in yeast
title_sort coupling among growth rate response, metabolic cycle, and cell division cycle in yeast
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3113766/
https://www.ncbi.nlm.nih.gov/pubmed/21525243
http://dx.doi.org/10.1091/mbc.E11-02-0132
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