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The RNA Editing Pattern of cox2 mRNA Is Affected by Point Mutations in Plant Mitochondria

The mitochondrial transcriptome from land plants undergoes hundreds of specific C-to-U changes by RNA editing. These events are important since most of them occur in the coding region of mRNAs. One challenging question is to understand the mechanism of recognition of a selected C residue (editing si...

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Autores principales: Castandet, Benoît, Araya, Alejandro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3113845/
https://www.ncbi.nlm.nih.gov/pubmed/21695137
http://dx.doi.org/10.1371/journal.pone.0020867
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author Castandet, Benoît
Araya, Alejandro
author_facet Castandet, Benoît
Araya, Alejandro
author_sort Castandet, Benoît
collection PubMed
description The mitochondrial transcriptome from land plants undergoes hundreds of specific C-to-U changes by RNA editing. These events are important since most of them occur in the coding region of mRNAs. One challenging question is to understand the mechanism of recognition of a selected C residue (editing sites) on the transcript. It has been reported that a short region surrounding the target C forms the cis-recognition elements, but individual residues on it do not play similar roles for the different editing sites. Here, we studied the role of the −1 and +1 nucleotide in wheat cox2 editing site recognition using an in organello approach. We found that four different recognition patterns can be distinguished: (a) +1 dependency, (b) −1 dependency, (c) +1/−1 dependency, and (d) no dependency on nearest neighbor residues. A striking observation was that whereas a 23 nt cis region is necessary for editing, some mutants affect the editing efficiency of unmodified distant sites. As a rule, mutations or pre-edited variants of the transcript have an impact on the complete set of editing targets. When some Cs were changed into Us, the remaining editing sites presented a higher efficiency of C-to-U conversion than in wild type mRNA. Our data suggest that the complex response observed for cox2 mRNA may be a consequence of the fate of the transcript during mitochondrial gene expression.
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spelling pubmed-31138452011-06-21 The RNA Editing Pattern of cox2 mRNA Is Affected by Point Mutations in Plant Mitochondria Castandet, Benoît Araya, Alejandro PLoS One Research Article The mitochondrial transcriptome from land plants undergoes hundreds of specific C-to-U changes by RNA editing. These events are important since most of them occur in the coding region of mRNAs. One challenging question is to understand the mechanism of recognition of a selected C residue (editing sites) on the transcript. It has been reported that a short region surrounding the target C forms the cis-recognition elements, but individual residues on it do not play similar roles for the different editing sites. Here, we studied the role of the −1 and +1 nucleotide in wheat cox2 editing site recognition using an in organello approach. We found that four different recognition patterns can be distinguished: (a) +1 dependency, (b) −1 dependency, (c) +1/−1 dependency, and (d) no dependency on nearest neighbor residues. A striking observation was that whereas a 23 nt cis region is necessary for editing, some mutants affect the editing efficiency of unmodified distant sites. As a rule, mutations or pre-edited variants of the transcript have an impact on the complete set of editing targets. When some Cs were changed into Us, the remaining editing sites presented a higher efficiency of C-to-U conversion than in wild type mRNA. Our data suggest that the complex response observed for cox2 mRNA may be a consequence of the fate of the transcript during mitochondrial gene expression. Public Library of Science 2011-06-13 /pmc/articles/PMC3113845/ /pubmed/21695137 http://dx.doi.org/10.1371/journal.pone.0020867 Text en Castandet, Araya. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Castandet, Benoît
Araya, Alejandro
The RNA Editing Pattern of cox2 mRNA Is Affected by Point Mutations in Plant Mitochondria
title The RNA Editing Pattern of cox2 mRNA Is Affected by Point Mutations in Plant Mitochondria
title_full The RNA Editing Pattern of cox2 mRNA Is Affected by Point Mutations in Plant Mitochondria
title_fullStr The RNA Editing Pattern of cox2 mRNA Is Affected by Point Mutations in Plant Mitochondria
title_full_unstemmed The RNA Editing Pattern of cox2 mRNA Is Affected by Point Mutations in Plant Mitochondria
title_short The RNA Editing Pattern of cox2 mRNA Is Affected by Point Mutations in Plant Mitochondria
title_sort rna editing pattern of cox2 mrna is affected by point mutations in plant mitochondria
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3113845/
https://www.ncbi.nlm.nih.gov/pubmed/21695137
http://dx.doi.org/10.1371/journal.pone.0020867
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