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The RNA Editing Pattern of cox2 mRNA Is Affected by Point Mutations in Plant Mitochondria
The mitochondrial transcriptome from land plants undergoes hundreds of specific C-to-U changes by RNA editing. These events are important since most of them occur in the coding region of mRNAs. One challenging question is to understand the mechanism of recognition of a selected C residue (editing si...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3113845/ https://www.ncbi.nlm.nih.gov/pubmed/21695137 http://dx.doi.org/10.1371/journal.pone.0020867 |
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author | Castandet, Benoît Araya, Alejandro |
author_facet | Castandet, Benoît Araya, Alejandro |
author_sort | Castandet, Benoît |
collection | PubMed |
description | The mitochondrial transcriptome from land plants undergoes hundreds of specific C-to-U changes by RNA editing. These events are important since most of them occur in the coding region of mRNAs. One challenging question is to understand the mechanism of recognition of a selected C residue (editing sites) on the transcript. It has been reported that a short region surrounding the target C forms the cis-recognition elements, but individual residues on it do not play similar roles for the different editing sites. Here, we studied the role of the −1 and +1 nucleotide in wheat cox2 editing site recognition using an in organello approach. We found that four different recognition patterns can be distinguished: (a) +1 dependency, (b) −1 dependency, (c) +1/−1 dependency, and (d) no dependency on nearest neighbor residues. A striking observation was that whereas a 23 nt cis region is necessary for editing, some mutants affect the editing efficiency of unmodified distant sites. As a rule, mutations or pre-edited variants of the transcript have an impact on the complete set of editing targets. When some Cs were changed into Us, the remaining editing sites presented a higher efficiency of C-to-U conversion than in wild type mRNA. Our data suggest that the complex response observed for cox2 mRNA may be a consequence of the fate of the transcript during mitochondrial gene expression. |
format | Online Article Text |
id | pubmed-3113845 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-31138452011-06-21 The RNA Editing Pattern of cox2 mRNA Is Affected by Point Mutations in Plant Mitochondria Castandet, Benoît Araya, Alejandro PLoS One Research Article The mitochondrial transcriptome from land plants undergoes hundreds of specific C-to-U changes by RNA editing. These events are important since most of them occur in the coding region of mRNAs. One challenging question is to understand the mechanism of recognition of a selected C residue (editing sites) on the transcript. It has been reported that a short region surrounding the target C forms the cis-recognition elements, but individual residues on it do not play similar roles for the different editing sites. Here, we studied the role of the −1 and +1 nucleotide in wheat cox2 editing site recognition using an in organello approach. We found that four different recognition patterns can be distinguished: (a) +1 dependency, (b) −1 dependency, (c) +1/−1 dependency, and (d) no dependency on nearest neighbor residues. A striking observation was that whereas a 23 nt cis region is necessary for editing, some mutants affect the editing efficiency of unmodified distant sites. As a rule, mutations or pre-edited variants of the transcript have an impact on the complete set of editing targets. When some Cs were changed into Us, the remaining editing sites presented a higher efficiency of C-to-U conversion than in wild type mRNA. Our data suggest that the complex response observed for cox2 mRNA may be a consequence of the fate of the transcript during mitochondrial gene expression. Public Library of Science 2011-06-13 /pmc/articles/PMC3113845/ /pubmed/21695137 http://dx.doi.org/10.1371/journal.pone.0020867 Text en Castandet, Araya. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Castandet, Benoît Araya, Alejandro The RNA Editing Pattern of cox2 mRNA Is Affected by Point Mutations in Plant Mitochondria |
title | The RNA Editing Pattern of cox2 mRNA Is Affected by Point Mutations in Plant Mitochondria |
title_full | The RNA Editing Pattern of cox2 mRNA Is Affected by Point Mutations in Plant Mitochondria |
title_fullStr | The RNA Editing Pattern of cox2 mRNA Is Affected by Point Mutations in Plant Mitochondria |
title_full_unstemmed | The RNA Editing Pattern of cox2 mRNA Is Affected by Point Mutations in Plant Mitochondria |
title_short | The RNA Editing Pattern of cox2 mRNA Is Affected by Point Mutations in Plant Mitochondria |
title_sort | rna editing pattern of cox2 mrna is affected by point mutations in plant mitochondria |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3113845/ https://www.ncbi.nlm.nih.gov/pubmed/21695137 http://dx.doi.org/10.1371/journal.pone.0020867 |
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