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Enhanced transfection of cell lines from Atlantic salmon through nucoleofection and antibiotic selection

BACKGROUND: Cell lines from Atlantic salmon kidney have made it possible to culture and study infectious salmon anemia virus (ISAV), an aquatic orthomyxovirus affecting farmed Atlantic salmon. However, transfection of these cells using calcium phosphate precipitation or lipid-based reagents shows ve...

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Detalles Bibliográficos
Autores principales:	Schiøtz, Berit L, Rosado, Esther G, Baekkevold, Espen S, Lukacs, Morten, Mjaaland, Siri, Sindre, Hilde, Grimholt, Unni, Gjøen, Tor
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2011
Materias:	Short Report
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3113957/ https://www.ncbi.nlm.nih.gov/pubmed/21548922 http://dx.doi.org/10.1186/1756-0500-4-136

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