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Near-IR fluorescence and reflectance confocal microscopy for imaging of quantum dots in mammalian skin

Understanding the skin penetration of nanoparticles (NPs) is an important concern due to the increasing presence of NPs in consumer products, including cosmetics. Technical challenges have slowed progress in evaluating skin barrier and NP factors that contribute to skin penetration risk. To limit sa...

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Autores principales: Mortensen, Luke J., Glazowski, Christopher E., Zavislan, James M., DeLouise, Lisa A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3114228/
https://www.ncbi.nlm.nih.gov/pubmed/21698023
http://dx.doi.org/10.1364/BOE.2.001610
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author Mortensen, Luke J.
Glazowski, Christopher E.
Zavislan, James M.
DeLouise, Lisa A.
author_facet Mortensen, Luke J.
Glazowski, Christopher E.
Zavislan, James M.
DeLouise, Lisa A.
author_sort Mortensen, Luke J.
collection PubMed
description Understanding the skin penetration of nanoparticles (NPs) is an important concern due to the increasing presence of NPs in consumer products, including cosmetics. Technical challenges have slowed progress in evaluating skin barrier and NP factors that contribute to skin penetration risk. To limit sampling error and other problems associated with histological processing, many researchers are implementing whole tissue confocal or multiphoton microscopies. This work introduces a fluorescence and reflectance confocal microscopy system that utilizes near-IR excitation and emission to detect near-IR lead sulfide quantum dots (QDs) through ex vivo human epidermis. We provide a detailed prediction and experimental analysis of QD detection sensitivity and demonstrate detection of QD skin penetration in a barrier disrupted model. The unique properties of near-IR lead-based QDs will enable future studies that examine the impact of further barrier-disrupting agents on skin penetration of QDs and elucidate mechanistic insight into QD tissue interactions at the cellular level.
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spelling pubmed-31142282011-06-22 Near-IR fluorescence and reflectance confocal microscopy for imaging of quantum dots in mammalian skin Mortensen, Luke J. Glazowski, Christopher E. Zavislan, James M. DeLouise, Lisa A. Biomed Opt Express Nanotechnology and Plasmonics Understanding the skin penetration of nanoparticles (NPs) is an important concern due to the increasing presence of NPs in consumer products, including cosmetics. Technical challenges have slowed progress in evaluating skin barrier and NP factors that contribute to skin penetration risk. To limit sampling error and other problems associated with histological processing, many researchers are implementing whole tissue confocal or multiphoton microscopies. This work introduces a fluorescence and reflectance confocal microscopy system that utilizes near-IR excitation and emission to detect near-IR lead sulfide quantum dots (QDs) through ex vivo human epidermis. We provide a detailed prediction and experimental analysis of QD detection sensitivity and demonstrate detection of QD skin penetration in a barrier disrupted model. The unique properties of near-IR lead-based QDs will enable future studies that examine the impact of further barrier-disrupting agents on skin penetration of QDs and elucidate mechanistic insight into QD tissue interactions at the cellular level. Optical Society of America 2011-05-19 /pmc/articles/PMC3114228/ /pubmed/21698023 http://dx.doi.org/10.1364/BOE.2.001610 Text en ©2011 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Nanotechnology and Plasmonics
Mortensen, Luke J.
Glazowski, Christopher E.
Zavislan, James M.
DeLouise, Lisa A.
Near-IR fluorescence and reflectance confocal microscopy for imaging of quantum dots in mammalian skin
title Near-IR fluorescence and reflectance confocal microscopy for imaging of quantum dots in mammalian skin
title_full Near-IR fluorescence and reflectance confocal microscopy for imaging of quantum dots in mammalian skin
title_fullStr Near-IR fluorescence and reflectance confocal microscopy for imaging of quantum dots in mammalian skin
title_full_unstemmed Near-IR fluorescence and reflectance confocal microscopy for imaging of quantum dots in mammalian skin
title_short Near-IR fluorescence and reflectance confocal microscopy for imaging of quantum dots in mammalian skin
title_sort near-ir fluorescence and reflectance confocal microscopy for imaging of quantum dots in mammalian skin
topic Nanotechnology and Plasmonics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3114228/
https://www.ncbi.nlm.nih.gov/pubmed/21698023
http://dx.doi.org/10.1364/BOE.2.001610
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