Cargando…

Karyotypic conservatism in samples of Characidium cf. zebra (Teleostei, Characiformes, Crenuchidae): Physical mapping of ribosomal genes and natural triploidy

Basic and molecular cytogenetic analyses were performed in specimens of Characidium cf. zebra from five collection sites located throughout the Tietê, Paranapanema and Paraguay river basins. The diploid number in specimens from all samples was 2n = 50 with a karyotype composed of 32 metacentric and...

Descripción completa

Detalles Bibliográficos
Autores principales: Pansonato-Alves, José Carlos, Oliveira, Claudio, Foresti, Fausto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Genética 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3115311/
https://www.ncbi.nlm.nih.gov/pubmed/21734818
http://dx.doi.org/10.1590/S1415-47572011005000005
_version_ 1782206149908496384
author Pansonato-Alves, José Carlos
Oliveira, Claudio
Foresti, Fausto
author_facet Pansonato-Alves, José Carlos
Oliveira, Claudio
Foresti, Fausto
author_sort Pansonato-Alves, José Carlos
collection PubMed
description Basic and molecular cytogenetic analyses were performed in specimens of Characidium cf. zebra from five collection sites located throughout the Tietê, Paranapanema and Paraguay river basins. The diploid number in specimens from all samples was 2n = 50 with a karyotype composed of 32 metacentric and 18 submetacentric chromosomes in both males and females. Constitutive heterochromatin was present at the centromeric regions of all chromosomes and pair 23, had additional interstitial heterochromatic blocks on its long arms. The nucleolar organizer regions (NORs) were located on the long arms of pair 23, while the 5S rDNA sites were detected in different chromosomes among the studied samples. One specimen from the Alambari river was a natural triploid and had two extra chromosomes, resulting in 2n = 77. The remarkable karyotypic similarity among the specimens of C. cf. zebra suggests a close evolutionary relationship. On the other hand, the distinct patterns of 5S rDNA distribution may be the result of gene flow constraints during their evolutionary history.
format Online
Article
Text
id pubmed-3115311
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Sociedade Brasileira de Genética
record_format MEDLINE/PubMed
spelling pubmed-31153112011-07-06 Karyotypic conservatism in samples of Characidium cf. zebra (Teleostei, Characiformes, Crenuchidae): Physical mapping of ribosomal genes and natural triploidy Pansonato-Alves, José Carlos Oliveira, Claudio Foresti, Fausto Genet Mol Biol Research Article Basic and molecular cytogenetic analyses were performed in specimens of Characidium cf. zebra from five collection sites located throughout the Tietê, Paranapanema and Paraguay river basins. The diploid number in specimens from all samples was 2n = 50 with a karyotype composed of 32 metacentric and 18 submetacentric chromosomes in both males and females. Constitutive heterochromatin was present at the centromeric regions of all chromosomes and pair 23, had additional interstitial heterochromatic blocks on its long arms. The nucleolar organizer regions (NORs) were located on the long arms of pair 23, while the 5S rDNA sites were detected in different chromosomes among the studied samples. One specimen from the Alambari river was a natural triploid and had two extra chromosomes, resulting in 2n = 77. The remarkable karyotypic similarity among the specimens of C. cf. zebra suggests a close evolutionary relationship. On the other hand, the distinct patterns of 5S rDNA distribution may be the result of gene flow constraints during their evolutionary history. Sociedade Brasileira de Genética 2011-04-01 2011 /pmc/articles/PMC3115311/ /pubmed/21734818 http://dx.doi.org/10.1590/S1415-47572011005000005 Text en Copyright © 2011, Sociedade Brasileira de Genética. Printed in Brazil License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Pansonato-Alves, José Carlos
Oliveira, Claudio
Foresti, Fausto
Karyotypic conservatism in samples of Characidium cf. zebra (Teleostei, Characiformes, Crenuchidae): Physical mapping of ribosomal genes and natural triploidy
title Karyotypic conservatism in samples of Characidium cf. zebra (Teleostei, Characiformes, Crenuchidae): Physical mapping of ribosomal genes and natural triploidy
title_full Karyotypic conservatism in samples of Characidium cf. zebra (Teleostei, Characiformes, Crenuchidae): Physical mapping of ribosomal genes and natural triploidy
title_fullStr Karyotypic conservatism in samples of Characidium cf. zebra (Teleostei, Characiformes, Crenuchidae): Physical mapping of ribosomal genes and natural triploidy
title_full_unstemmed Karyotypic conservatism in samples of Characidium cf. zebra (Teleostei, Characiformes, Crenuchidae): Physical mapping of ribosomal genes and natural triploidy
title_short Karyotypic conservatism in samples of Characidium cf. zebra (Teleostei, Characiformes, Crenuchidae): Physical mapping of ribosomal genes and natural triploidy
title_sort karyotypic conservatism in samples of characidium cf. zebra (teleostei, characiformes, crenuchidae): physical mapping of ribosomal genes and natural triploidy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3115311/
https://www.ncbi.nlm.nih.gov/pubmed/21734818
http://dx.doi.org/10.1590/S1415-47572011005000005
work_keys_str_mv AT pansonatoalvesjosecarlos karyotypicconservatisminsamplesofcharacidiumcfzebrateleosteicharaciformescrenuchidaephysicalmappingofribosomalgenesandnaturaltriploidy
AT oliveiraclaudio karyotypicconservatisminsamplesofcharacidiumcfzebrateleosteicharaciformescrenuchidaephysicalmappingofribosomalgenesandnaturaltriploidy
AT forestifausto karyotypicconservatisminsamplesofcharacidiumcfzebrateleosteicharaciformescrenuchidaephysicalmappingofribosomalgenesandnaturaltriploidy