Isolation and multiplex genotyping of polymorphic microsatellite DNA markers in the snakehead murrel, Channa striata

Seven polymorphic microsatellite loci were isolated and characterized for the snakehead murrel, Channa striata (Channidae), a valuable tropical freshwater fish species. Among 25 specimens collected from Kedah state in Malaysia, the number of alleles per locus ranged from 2 to 7. Observed and expecte...

Descripción completa

Detalles Bibliográficos
Autores principales: Jamsari, Amirul Firdaus Jamaluddin, Min-Pau, Tan, Siti-Azizah, Mohd Nor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Genética 2011
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3115333/
https://www.ncbi.nlm.nih.gov/pubmed/21734840
http://dx.doi.org/10.1590/S1415-4757201100500000
_version_ 1782206155067490304
author Jamsari, Amirul Firdaus Jamaluddin
Min-Pau, Tan
Siti-Azizah, Mohd Nor
author_facet Jamsari, Amirul Firdaus Jamaluddin
Min-Pau, Tan
Siti-Azizah, Mohd Nor
author_sort Jamsari, Amirul Firdaus Jamaluddin
collection PubMed
description Seven polymorphic microsatellite loci were isolated and characterized for the snakehead murrel, Channa striata (Channidae), a valuable tropical freshwater fish species. Among 25 specimens collected from Kedah state in Malaysia, the number of alleles per locus ranged from 2 to 7. Observed and expected heterozygosities ranged from 0.120 to 0.880 and 0.117 to 0.698, respectively. A single locus (CS1-C07) was significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction. These novel markers would be useful for population genetic studies of the C. striata.
format Online
Article
Text
id pubmed-3115333
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Sociedade Brasileira de Genética
record_format MEDLINE/PubMed
spelling pubmed-31153332011-07-06 Isolation and multiplex genotyping of polymorphic microsatellite DNA markers in the snakehead murrel, Channa striata Jamsari, Amirul Firdaus Jamaluddin Min-Pau, Tan Siti-Azizah, Mohd Nor Genet Mol Biol Short Communication Seven polymorphic microsatellite loci were isolated and characterized for the snakehead murrel, Channa striata (Channidae), a valuable tropical freshwater fish species. Among 25 specimens collected from Kedah state in Malaysia, the number of alleles per locus ranged from 2 to 7. Observed and expected heterozygosities ranged from 0.120 to 0.880 and 0.117 to 0.698, respectively. A single locus (CS1-C07) was significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction. These novel markers would be useful for population genetic studies of the C. striata. Sociedade Brasileira de Genética 2011-04-01 2011 /pmc/articles/PMC3115333/ /pubmed/21734840 http://dx.doi.org/10.1590/S1415-4757201100500000 Text en Copyright © 2011, Sociedade Brasileira de Genética. Printed in Brazil License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Jamsari, Amirul Firdaus Jamaluddin
Min-Pau, Tan
Siti-Azizah, Mohd Nor
Isolation and multiplex genotyping of polymorphic microsatellite DNA markers in the snakehead murrel, Channa striata
title Isolation and multiplex genotyping of polymorphic microsatellite DNA markers in the snakehead murrel, Channa striata
title_full Isolation and multiplex genotyping of polymorphic microsatellite DNA markers in the snakehead murrel, Channa striata
title_fullStr Isolation and multiplex genotyping of polymorphic microsatellite DNA markers in the snakehead murrel, Channa striata
title_full_unstemmed Isolation and multiplex genotyping of polymorphic microsatellite DNA markers in the snakehead murrel, Channa striata
title_short Isolation and multiplex genotyping of polymorphic microsatellite DNA markers in the snakehead murrel, Channa striata
title_sort isolation and multiplex genotyping of polymorphic microsatellite dna markers in the snakehead murrel, channa striata
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3115333/
https://www.ncbi.nlm.nih.gov/pubmed/21734840
http://dx.doi.org/10.1590/S1415-4757201100500000
work_keys_str_mv AT jamsariamirulfirdausjamaluddin isolationandmultiplexgenotypingofpolymorphicmicrosatellitednamarkersinthesnakeheadmurrelchannastriata
AT minpautan isolationandmultiplexgenotypingofpolymorphicmicrosatellitednamarkersinthesnakeheadmurrelchannastriata
AT sitiazizahmohdnor isolationandmultiplexgenotypingofpolymorphicmicrosatellitednamarkersinthesnakeheadmurrelchannastriata

Ejemplares similares