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Reliability of Serum Metabolite Concentrations over a 4-Month Period Using a Targeted Metabolomic Approach
Metabolomics is a promising tool for discovery of novel biomarkers of chronic disease risk in prospective epidemiologic studies. We investigated the between- and within-person variation of the concentrations of 163 serum metabolites over a period of 4 months to evaluate the metabolite reliability ex...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3115978/ https://www.ncbi.nlm.nih.gov/pubmed/21698256 http://dx.doi.org/10.1371/journal.pone.0021103 |
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author | Floegel, Anna Drogan, Dagmar Wang-Sattler, Rui Prehn, Cornelia Illig, Thomas Adamski, Jerzy Joost, Hans-Georg Boeing, Heiner Pischon, Tobias |
author_facet | Floegel, Anna Drogan, Dagmar Wang-Sattler, Rui Prehn, Cornelia Illig, Thomas Adamski, Jerzy Joost, Hans-Georg Boeing, Heiner Pischon, Tobias |
author_sort | Floegel, Anna |
collection | PubMed |
description | Metabolomics is a promising tool for discovery of novel biomarkers of chronic disease risk in prospective epidemiologic studies. We investigated the between- and within-person variation of the concentrations of 163 serum metabolites over a period of 4 months to evaluate the metabolite reliability expressed by the intraclass-correlation coefficient (ICC: the ratio of between-person variance and total variance). The analyses were performed with the BIOCRATES AbsoluteIDQ™ targeted metabolomics technology, including acylcarnitines, amino acids, glycerophospholipids, sphingolipids and hexose in 100 healthy individuals from the European Prospective Investigation into Cancer and Nutrition (EPIC)-Potsdam study who had provided two fasting blood samples 4 months apart. Overall, serum reliability of metabolites over a 4-month period was good. The median ICC of the 163 metabolites was 0.57. The highest ICC was observed for hydroxysphingomyelin C14:1 (ICC = 0.85) and the lowest was found for acylcarnitine C3:1 (ICC = 0). Reliability was high for hexose (ICC = 0.76), sphingolipids (median ICC = 0.66; range: 0.24–0.85), amino acids (median ICC = 0.58; range: 0.41–0.72) and glycerophospholipids (median ICC = 0.58; range: 0.03–0.81). Among acylcarnitines, reliability of short and medium chain saturated compounds was good to excellent (ICC range: 0.50–0.81). Serum reliability was lower for most hydroxyacylcarnitines and monounsaturated acylcarnitines (ICC range: 0.11–0.45 and 0.00–0.63, respectively). For most of the metabolites a single measurement may be sufficient for risk assessment in epidemiologic studies with healthy subjects. |
format | Online Article Text |
id | pubmed-3115978 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-31159782011-06-22 Reliability of Serum Metabolite Concentrations over a 4-Month Period Using a Targeted Metabolomic Approach Floegel, Anna Drogan, Dagmar Wang-Sattler, Rui Prehn, Cornelia Illig, Thomas Adamski, Jerzy Joost, Hans-Georg Boeing, Heiner Pischon, Tobias PLoS One Research Article Metabolomics is a promising tool for discovery of novel biomarkers of chronic disease risk in prospective epidemiologic studies. We investigated the between- and within-person variation of the concentrations of 163 serum metabolites over a period of 4 months to evaluate the metabolite reliability expressed by the intraclass-correlation coefficient (ICC: the ratio of between-person variance and total variance). The analyses were performed with the BIOCRATES AbsoluteIDQ™ targeted metabolomics technology, including acylcarnitines, amino acids, glycerophospholipids, sphingolipids and hexose in 100 healthy individuals from the European Prospective Investigation into Cancer and Nutrition (EPIC)-Potsdam study who had provided two fasting blood samples 4 months apart. Overall, serum reliability of metabolites over a 4-month period was good. The median ICC of the 163 metabolites was 0.57. The highest ICC was observed for hydroxysphingomyelin C14:1 (ICC = 0.85) and the lowest was found for acylcarnitine C3:1 (ICC = 0). Reliability was high for hexose (ICC = 0.76), sphingolipids (median ICC = 0.66; range: 0.24–0.85), amino acids (median ICC = 0.58; range: 0.41–0.72) and glycerophospholipids (median ICC = 0.58; range: 0.03–0.81). Among acylcarnitines, reliability of short and medium chain saturated compounds was good to excellent (ICC range: 0.50–0.81). Serum reliability was lower for most hydroxyacylcarnitines and monounsaturated acylcarnitines (ICC range: 0.11–0.45 and 0.00–0.63, respectively). For most of the metabolites a single measurement may be sufficient for risk assessment in epidemiologic studies with healthy subjects. Public Library of Science 2011-06-15 /pmc/articles/PMC3115978/ /pubmed/21698256 http://dx.doi.org/10.1371/journal.pone.0021103 Text en Floegel et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Floegel, Anna Drogan, Dagmar Wang-Sattler, Rui Prehn, Cornelia Illig, Thomas Adamski, Jerzy Joost, Hans-Georg Boeing, Heiner Pischon, Tobias Reliability of Serum Metabolite Concentrations over a 4-Month Period Using a Targeted Metabolomic Approach |
title | Reliability of Serum Metabolite Concentrations over a 4-Month Period Using a Targeted Metabolomic Approach |
title_full | Reliability of Serum Metabolite Concentrations over a 4-Month Period Using a Targeted Metabolomic Approach |
title_fullStr | Reliability of Serum Metabolite Concentrations over a 4-Month Period Using a Targeted Metabolomic Approach |
title_full_unstemmed | Reliability of Serum Metabolite Concentrations over a 4-Month Period Using a Targeted Metabolomic Approach |
title_short | Reliability of Serum Metabolite Concentrations over a 4-Month Period Using a Targeted Metabolomic Approach |
title_sort | reliability of serum metabolite concentrations over a 4-month period using a targeted metabolomic approach |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3115978/ https://www.ncbi.nlm.nih.gov/pubmed/21698256 http://dx.doi.org/10.1371/journal.pone.0021103 |
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