Determination of Real-Time Efflux Phenotypes in Escherichia coli AcrB Binding Pocket Phenylalanine Mutants Using a 1,2′-Dinaphthylamine Efflux Assay

To evaluate the importance of phenylalanine residues for substrate transport in the Escherichia coli efflux pump protein AcrB, we subjected Phe-to-Ala binding pocket mutants to a real-time efflux assay with the novel near-infrared lipophilic membrane probe 1,2′-dinaphthylamine (1,2′-DNA). All mutati...

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Detalles Bibliográficos
Autores principales: Bohnert, Jürgen A., Schuster, Sabine, Szymaniak-Vits, Magdalena, Kern, Winfried V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3115983/
https://www.ncbi.nlm.nih.gov/pubmed/21698261
http://dx.doi.org/10.1371/journal.pone.0021196

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