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In vivo microRNA-155 expression influences antigen-specific T cell-mediated immune responses generated by DNA vaccination
BACKGROUND: MicroRNA (miRNA) molecules are potent mediators of post-transcriptional gene silencing that are emerging to be critical in the regulation of innate and adaptive immunity. RESULTS: Here we report that miR-155--an oncogenic miRNA with important function in the mammalian immune system--is i...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3116247/ https://www.ncbi.nlm.nih.gov/pubmed/21711593 http://dx.doi.org/10.1186/2045-3701-1-3 |
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author | Mao, Chih-Ping He, Liangmei Tsai, Ya-Chea Peng, Shiwen Kang, Tae Heung Pang, Xiaowu Monie, Archana Hung, Chien-Fu Wu, T-C |
author_facet | Mao, Chih-Ping He, Liangmei Tsai, Ya-Chea Peng, Shiwen Kang, Tae Heung Pang, Xiaowu Monie, Archana Hung, Chien-Fu Wu, T-C |
author_sort | Mao, Chih-Ping |
collection | PubMed |
description | BACKGROUND: MicroRNA (miRNA) molecules are potent mediators of post-transcriptional gene silencing that are emerging to be critical in the regulation of innate and adaptive immunity. RESULTS: Here we report that miR-155--an oncogenic miRNA with important function in the mammalian immune system--is induced in dendritic cells (DCs) upon maturation and potentially attenuates their ability to activate T cells. Biolistic epidermal transfection with DNA encoding miR-155 suppressed the induction of antigen-specific T cell-mediated immunity, whereas reduction of endogenous miR-155 by a partially complementary antisense sequence reversed this effect. Because DCs represent a significant component of epidermal tissue and are among the most potent of antigen-presenting cells, the inhibitory actions of miR-155 could be mediated through this subset of cells. CONCLUSIONS: These results suggest that miR-155 may repress the expression of key molecules involved in lymph node migration, antigen presentation, or T cell activation in DCs, and thus forms part of a negative regulatory pathway that dampens the generation of T cell-mediated immune responses. Modulation of miR-155 expression in epidermis therefore represents a potentially promising form of gene therapy for the control of diseases ranging from autoimmunity to cancer and viral infection. |
format | Online Article Text |
id | pubmed-3116247 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-31162472011-06-16 In vivo microRNA-155 expression influences antigen-specific T cell-mediated immune responses generated by DNA vaccination Mao, Chih-Ping He, Liangmei Tsai, Ya-Chea Peng, Shiwen Kang, Tae Heung Pang, Xiaowu Monie, Archana Hung, Chien-Fu Wu, T-C Cell Biosci Research BACKGROUND: MicroRNA (miRNA) molecules are potent mediators of post-transcriptional gene silencing that are emerging to be critical in the regulation of innate and adaptive immunity. RESULTS: Here we report that miR-155--an oncogenic miRNA with important function in the mammalian immune system--is induced in dendritic cells (DCs) upon maturation and potentially attenuates their ability to activate T cells. Biolistic epidermal transfection with DNA encoding miR-155 suppressed the induction of antigen-specific T cell-mediated immunity, whereas reduction of endogenous miR-155 by a partially complementary antisense sequence reversed this effect. Because DCs represent a significant component of epidermal tissue and are among the most potent of antigen-presenting cells, the inhibitory actions of miR-155 could be mediated through this subset of cells. CONCLUSIONS: These results suggest that miR-155 may repress the expression of key molecules involved in lymph node migration, antigen presentation, or T cell activation in DCs, and thus forms part of a negative regulatory pathway that dampens the generation of T cell-mediated immune responses. Modulation of miR-155 expression in epidermis therefore represents a potentially promising form of gene therapy for the control of diseases ranging from autoimmunity to cancer and viral infection. BioMed Central 2011-01-18 /pmc/articles/PMC3116247/ /pubmed/21711593 http://dx.doi.org/10.1186/2045-3701-1-3 Text en Copyright ©2011 Mao et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Mao, Chih-Ping He, Liangmei Tsai, Ya-Chea Peng, Shiwen Kang, Tae Heung Pang, Xiaowu Monie, Archana Hung, Chien-Fu Wu, T-C In vivo microRNA-155 expression influences antigen-specific T cell-mediated immune responses generated by DNA vaccination |
title | In vivo microRNA-155 expression influences antigen-specific T cell-mediated immune responses generated by DNA vaccination |
title_full | In vivo microRNA-155 expression influences antigen-specific T cell-mediated immune responses generated by DNA vaccination |
title_fullStr | In vivo microRNA-155 expression influences antigen-specific T cell-mediated immune responses generated by DNA vaccination |
title_full_unstemmed | In vivo microRNA-155 expression influences antigen-specific T cell-mediated immune responses generated by DNA vaccination |
title_short | In vivo microRNA-155 expression influences antigen-specific T cell-mediated immune responses generated by DNA vaccination |
title_sort | in vivo microrna-155 expression influences antigen-specific t cell-mediated immune responses generated by dna vaccination |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3116247/ https://www.ncbi.nlm.nih.gov/pubmed/21711593 http://dx.doi.org/10.1186/2045-3701-1-3 |
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