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Lack of Processing of the Expressed ORF1 Gene Product of Hepatitis E Virus

BACKGROUND: Proteolytic processing is a common mechanism among plus strand RNA viruses and the replicases of all plus strand RNA viruses of animals thus far characterized undergo such processing. The replicase proteins of hepatitis E virus (HEV) are encoded by ORF1. A previous report published by ou...

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Autores principales: Suppiah, Suganthi, Zhou, Yumei, Frey, Teryl K
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3116494/
https://www.ncbi.nlm.nih.gov/pubmed/21595991
http://dx.doi.org/10.1186/1743-422X-8-245
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author Suppiah, Suganthi
Zhou, Yumei
Frey, Teryl K
author_facet Suppiah, Suganthi
Zhou, Yumei
Frey, Teryl K
author_sort Suppiah, Suganthi
collection PubMed
description BACKGROUND: Proteolytic processing is a common mechanism among plus strand RNA viruses and the replicases of all plus strand RNA viruses of animals thus far characterized undergo such processing. The replicase proteins of hepatitis E virus (HEV) are encoded by ORF1. A previous report published by our group [1] provided data that processing potentially occurred when ORF1 (Burma strain; genotype 1) was expressed using a vaccinia virus-based expression system. FINDINGS: To further test for processing and to rule out artifacts associated with the expression system, ORF1 was re-expressed using a plasmid-based expression vector with the result that the previous processing profile could not be confirmed. When ORF1 from an HEV infectious cDNA clone (US swine strain; genotype 3) was expressed using the plasmid-based system, the only species detected was the 185 kDa precursor of ORF1. A putative papain-like cysteine protease [2] had been predicted within ORF1 using the original HEV genomic sequence. However, analysis of subsequent ORF1 sequences from a large number of HEV isolates reveals that this protease motif is not conserved. CONCLUSIONS: The expressed HEV ORF1 gene product does not undergo proteolytic processing, indicating that the replicase precursor of HEV is potentially unique in this regard.
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spelling pubmed-31164942011-06-17 Lack of Processing of the Expressed ORF1 Gene Product of Hepatitis E Virus Suppiah, Suganthi Zhou, Yumei Frey, Teryl K Virol J Short Report BACKGROUND: Proteolytic processing is a common mechanism among plus strand RNA viruses and the replicases of all plus strand RNA viruses of animals thus far characterized undergo such processing. The replicase proteins of hepatitis E virus (HEV) are encoded by ORF1. A previous report published by our group [1] provided data that processing potentially occurred when ORF1 (Burma strain; genotype 1) was expressed using a vaccinia virus-based expression system. FINDINGS: To further test for processing and to rule out artifacts associated with the expression system, ORF1 was re-expressed using a plasmid-based expression vector with the result that the previous processing profile could not be confirmed. When ORF1 from an HEV infectious cDNA clone (US swine strain; genotype 3) was expressed using the plasmid-based system, the only species detected was the 185 kDa precursor of ORF1. A putative papain-like cysteine protease [2] had been predicted within ORF1 using the original HEV genomic sequence. However, analysis of subsequent ORF1 sequences from a large number of HEV isolates reveals that this protease motif is not conserved. CONCLUSIONS: The expressed HEV ORF1 gene product does not undergo proteolytic processing, indicating that the replicase precursor of HEV is potentially unique in this regard. BioMed Central 2011-05-20 /pmc/articles/PMC3116494/ /pubmed/21595991 http://dx.doi.org/10.1186/1743-422X-8-245 Text en Copyright ©2011 Suppiah et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Report
Suppiah, Suganthi
Zhou, Yumei
Frey, Teryl K
Lack of Processing of the Expressed ORF1 Gene Product of Hepatitis E Virus
title Lack of Processing of the Expressed ORF1 Gene Product of Hepatitis E Virus
title_full Lack of Processing of the Expressed ORF1 Gene Product of Hepatitis E Virus
title_fullStr Lack of Processing of the Expressed ORF1 Gene Product of Hepatitis E Virus
title_full_unstemmed Lack of Processing of the Expressed ORF1 Gene Product of Hepatitis E Virus
title_short Lack of Processing of the Expressed ORF1 Gene Product of Hepatitis E Virus
title_sort lack of processing of the expressed orf1 gene product of hepatitis e virus
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3116494/
https://www.ncbi.nlm.nih.gov/pubmed/21595991
http://dx.doi.org/10.1186/1743-422X-8-245
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