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A distinctive role for focal adhesion proteins in three-dimensional cell motility
Focal adhesions are large multi-protein assemblies that form at the basal surface of cells on planar dishes, which mediate cell signaling, force transduction, and adhesion to the substratum. While much is known about focal adhesion components in 2-D systems, their role in migrating cells within a mo...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3116660/ https://www.ncbi.nlm.nih.gov/pubmed/20473295 http://dx.doi.org/10.1038/ncb2062 |
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author | Fraley, Stephanie I. Feng, Yunfeng Krishnamurthy, Ranjini Kim, Dong-Hwee Celedon, Alfredo Longmore, Gregory D. Wirtz, Denis |
author_facet | Fraley, Stephanie I. Feng, Yunfeng Krishnamurthy, Ranjini Kim, Dong-Hwee Celedon, Alfredo Longmore, Gregory D. Wirtz, Denis |
author_sort | Fraley, Stephanie I. |
collection | PubMed |
description | Focal adhesions are large multi-protein assemblies that form at the basal surface of cells on planar dishes, which mediate cell signaling, force transduction, and adhesion to the substratum. While much is known about focal adhesion components in 2-D systems, their role in migrating cells within a more physiological three-dimensional (3-D) matrix is largely unknown. Live-cell microscopy shows that for cells fully embedded in a 3-D matrix, focal adhesion proteins, including vinculin, paxillin, talin, α-actinin, zyxin, VASP, FAK, and p130Cas, do not form aggregates but are diffusively distributed throughout the cytoplasm. Despite the absence of detectable focal adhesions, focal adhesion proteins still modulate cell motility but in a manner distinct from cells on planar substrates. Rather, focal adhesion proteins in matrix-embedded cells regulate cell speed and persistence by affecting protrusion activity and matrix deformation, two processes that play no direct role in controlling 2-D cell speed. This study shows that membrane protrusions constitute a critical motility/matrix-traction module that drives cell motility in a 3-D matrix. |
format | Online Article Text |
id | pubmed-3116660 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
record_format | MEDLINE/PubMed |
spelling | pubmed-31166602011-06-16 A distinctive role for focal adhesion proteins in three-dimensional cell motility Fraley, Stephanie I. Feng, Yunfeng Krishnamurthy, Ranjini Kim, Dong-Hwee Celedon, Alfredo Longmore, Gregory D. Wirtz, Denis Nat Cell Biol Article Focal adhesions are large multi-protein assemblies that form at the basal surface of cells on planar dishes, which mediate cell signaling, force transduction, and adhesion to the substratum. While much is known about focal adhesion components in 2-D systems, their role in migrating cells within a more physiological three-dimensional (3-D) matrix is largely unknown. Live-cell microscopy shows that for cells fully embedded in a 3-D matrix, focal adhesion proteins, including vinculin, paxillin, talin, α-actinin, zyxin, VASP, FAK, and p130Cas, do not form aggregates but are diffusively distributed throughout the cytoplasm. Despite the absence of detectable focal adhesions, focal adhesion proteins still modulate cell motility but in a manner distinct from cells on planar substrates. Rather, focal adhesion proteins in matrix-embedded cells regulate cell speed and persistence by affecting protrusion activity and matrix deformation, two processes that play no direct role in controlling 2-D cell speed. This study shows that membrane protrusions constitute a critical motility/matrix-traction module that drives cell motility in a 3-D matrix. 2010-05-16 2010-06 /pmc/articles/PMC3116660/ /pubmed/20473295 http://dx.doi.org/10.1038/ncb2062 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Fraley, Stephanie I. Feng, Yunfeng Krishnamurthy, Ranjini Kim, Dong-Hwee Celedon, Alfredo Longmore, Gregory D. Wirtz, Denis A distinctive role for focal adhesion proteins in three-dimensional cell motility |
title | A distinctive role for focal adhesion proteins in three-dimensional
cell motility |
title_full | A distinctive role for focal adhesion proteins in three-dimensional
cell motility |
title_fullStr | A distinctive role for focal adhesion proteins in three-dimensional
cell motility |
title_full_unstemmed | A distinctive role for focal adhesion proteins in three-dimensional
cell motility |
title_short | A distinctive role for focal adhesion proteins in three-dimensional
cell motility |
title_sort | distinctive role for focal adhesion proteins in three-dimensional
cell motility |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3116660/ https://www.ncbi.nlm.nih.gov/pubmed/20473295 http://dx.doi.org/10.1038/ncb2062 |
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