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A distinctive role for focal adhesion proteins in three-dimensional cell motility

Focal adhesions are large multi-protein assemblies that form at the basal surface of cells on planar dishes, which mediate cell signaling, force transduction, and adhesion to the substratum. While much is known about focal adhesion components in 2-D systems, their role in migrating cells within a mo...

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Autores principales: Fraley, Stephanie I., Feng, Yunfeng, Krishnamurthy, Ranjini, Kim, Dong-Hwee, Celedon, Alfredo, Longmore, Gregory D., Wirtz, Denis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3116660/
https://www.ncbi.nlm.nih.gov/pubmed/20473295
http://dx.doi.org/10.1038/ncb2062
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author Fraley, Stephanie I.
Feng, Yunfeng
Krishnamurthy, Ranjini
Kim, Dong-Hwee
Celedon, Alfredo
Longmore, Gregory D.
Wirtz, Denis
author_facet Fraley, Stephanie I.
Feng, Yunfeng
Krishnamurthy, Ranjini
Kim, Dong-Hwee
Celedon, Alfredo
Longmore, Gregory D.
Wirtz, Denis
author_sort Fraley, Stephanie I.
collection PubMed
description Focal adhesions are large multi-protein assemblies that form at the basal surface of cells on planar dishes, which mediate cell signaling, force transduction, and adhesion to the substratum. While much is known about focal adhesion components in 2-D systems, their role in migrating cells within a more physiological three-dimensional (3-D) matrix is largely unknown. Live-cell microscopy shows that for cells fully embedded in a 3-D matrix, focal adhesion proteins, including vinculin, paxillin, talin, α-actinin, zyxin, VASP, FAK, and p130Cas, do not form aggregates but are diffusively distributed throughout the cytoplasm. Despite the absence of detectable focal adhesions, focal adhesion proteins still modulate cell motility but in a manner distinct from cells on planar substrates. Rather, focal adhesion proteins in matrix-embedded cells regulate cell speed and persistence by affecting protrusion activity and matrix deformation, two processes that play no direct role in controlling 2-D cell speed. This study shows that membrane protrusions constitute a critical motility/matrix-traction module that drives cell motility in a 3-D matrix.
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spelling pubmed-31166602011-06-16 A distinctive role for focal adhesion proteins in three-dimensional cell motility Fraley, Stephanie I. Feng, Yunfeng Krishnamurthy, Ranjini Kim, Dong-Hwee Celedon, Alfredo Longmore, Gregory D. Wirtz, Denis Nat Cell Biol Article Focal adhesions are large multi-protein assemblies that form at the basal surface of cells on planar dishes, which mediate cell signaling, force transduction, and adhesion to the substratum. While much is known about focal adhesion components in 2-D systems, their role in migrating cells within a more physiological three-dimensional (3-D) matrix is largely unknown. Live-cell microscopy shows that for cells fully embedded in a 3-D matrix, focal adhesion proteins, including vinculin, paxillin, talin, α-actinin, zyxin, VASP, FAK, and p130Cas, do not form aggregates but are diffusively distributed throughout the cytoplasm. Despite the absence of detectable focal adhesions, focal adhesion proteins still modulate cell motility but in a manner distinct from cells on planar substrates. Rather, focal adhesion proteins in matrix-embedded cells regulate cell speed and persistence by affecting protrusion activity and matrix deformation, two processes that play no direct role in controlling 2-D cell speed. This study shows that membrane protrusions constitute a critical motility/matrix-traction module that drives cell motility in a 3-D matrix. 2010-05-16 2010-06 /pmc/articles/PMC3116660/ /pubmed/20473295 http://dx.doi.org/10.1038/ncb2062 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Fraley, Stephanie I.
Feng, Yunfeng
Krishnamurthy, Ranjini
Kim, Dong-Hwee
Celedon, Alfredo
Longmore, Gregory D.
Wirtz, Denis
A distinctive role for focal adhesion proteins in three-dimensional cell motility
title A distinctive role for focal adhesion proteins in three-dimensional cell motility
title_full A distinctive role for focal adhesion proteins in three-dimensional cell motility
title_fullStr A distinctive role for focal adhesion proteins in three-dimensional cell motility
title_full_unstemmed A distinctive role for focal adhesion proteins in three-dimensional cell motility
title_short A distinctive role for focal adhesion proteins in three-dimensional cell motility
title_sort distinctive role for focal adhesion proteins in three-dimensional cell motility
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3116660/
https://www.ncbi.nlm.nih.gov/pubmed/20473295
http://dx.doi.org/10.1038/ncb2062
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