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Characterization of the Diversity and Temporal Stability of Bacterial Communities in Human Milk

Recent investigations have demonstrated that human milk contains a variety of bacterial genera; however, as of yet very little work has been done to characterize the full diversity of these milk bacterial communities and their relative stability over time. To more thoroughly investigate the human mi...

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Autores principales: Hunt, Katherine M., Foster, James A., Forney, Larry J., Schütte, Ursel M. E., Beck, Daniel L., Abdo, Zaid, Fox, Lawrence K., Williams, Janet E., McGuire, Michelle K., McGuire, Mark A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3117882/
https://www.ncbi.nlm.nih.gov/pubmed/21695057
http://dx.doi.org/10.1371/journal.pone.0021313
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author Hunt, Katherine M.
Foster, James A.
Forney, Larry J.
Schütte, Ursel M. E.
Beck, Daniel L.
Abdo, Zaid
Fox, Lawrence K.
Williams, Janet E.
McGuire, Michelle K.
McGuire, Mark A.
author_facet Hunt, Katherine M.
Foster, James A.
Forney, Larry J.
Schütte, Ursel M. E.
Beck, Daniel L.
Abdo, Zaid
Fox, Lawrence K.
Williams, Janet E.
McGuire, Michelle K.
McGuire, Mark A.
author_sort Hunt, Katherine M.
collection PubMed
description Recent investigations have demonstrated that human milk contains a variety of bacterial genera; however, as of yet very little work has been done to characterize the full diversity of these milk bacterial communities and their relative stability over time. To more thoroughly investigate the human milk microbiome, we utilized microbial identification techniques based on pyrosequencing of the 16S ribosomal RNA gene. Specifically, we characterized the bacterial communities present in milk samples collected from 16 women at three time-points over four weeks. Results indicated that milk bacterial communities were generally complex; several genera represented greater than 5% of the relative community abundance, and the community was often, yet not always, stable over time within an individual. These results support the conclusion that human milk, which is recommended as the optimal nutrition source for almost all healthy infants, contains a collection of bacteria more diverse than previously reported. This finding begs the question as to what role this community plays in colonization of the infant gastrointestinal tract and maintaining mammary health.
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spelling pubmed-31178822011-06-21 Characterization of the Diversity and Temporal Stability of Bacterial Communities in Human Milk Hunt, Katherine M. Foster, James A. Forney, Larry J. Schütte, Ursel M. E. Beck, Daniel L. Abdo, Zaid Fox, Lawrence K. Williams, Janet E. McGuire, Michelle K. McGuire, Mark A. PLoS One Research Article Recent investigations have demonstrated that human milk contains a variety of bacterial genera; however, as of yet very little work has been done to characterize the full diversity of these milk bacterial communities and their relative stability over time. To more thoroughly investigate the human milk microbiome, we utilized microbial identification techniques based on pyrosequencing of the 16S ribosomal RNA gene. Specifically, we characterized the bacterial communities present in milk samples collected from 16 women at three time-points over four weeks. Results indicated that milk bacterial communities were generally complex; several genera represented greater than 5% of the relative community abundance, and the community was often, yet not always, stable over time within an individual. These results support the conclusion that human milk, which is recommended as the optimal nutrition source for almost all healthy infants, contains a collection of bacteria more diverse than previously reported. This finding begs the question as to what role this community plays in colonization of the infant gastrointestinal tract and maintaining mammary health. Public Library of Science 2011-06-17 /pmc/articles/PMC3117882/ /pubmed/21695057 http://dx.doi.org/10.1371/journal.pone.0021313 Text en Hunt et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Hunt, Katherine M.
Foster, James A.
Forney, Larry J.
Schütte, Ursel M. E.
Beck, Daniel L.
Abdo, Zaid
Fox, Lawrence K.
Williams, Janet E.
McGuire, Michelle K.
McGuire, Mark A.
Characterization of the Diversity and Temporal Stability of Bacterial Communities in Human Milk
title Characterization of the Diversity and Temporal Stability of Bacterial Communities in Human Milk
title_full Characterization of the Diversity and Temporal Stability of Bacterial Communities in Human Milk
title_fullStr Characterization of the Diversity and Temporal Stability of Bacterial Communities in Human Milk
title_full_unstemmed Characterization of the Diversity and Temporal Stability of Bacterial Communities in Human Milk
title_short Characterization of the Diversity and Temporal Stability of Bacterial Communities in Human Milk
title_sort characterization of the diversity and temporal stability of bacterial communities in human milk
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3117882/
https://www.ncbi.nlm.nih.gov/pubmed/21695057
http://dx.doi.org/10.1371/journal.pone.0021313
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