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The Lactobacillus flora in vagina and rectum of fertile and postmenopausal healthy Swedish women

BACKGROUND: Lactobacillus species are the most often found inhabitants of vaginal ecosystem of fertile women. In postmenopausal women with low oestrogen levels, Lactobacillus flora is diminishing or absent. However, no studies have been performed to investigate the correlation between oestrogen leve...

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Autores principales: Gustafsson, Rita J, Ahrné, Siv, Jeppsson, Bengt, Benoni, Cecilia, Olsson, Crister, Stjernquist, Martin, Ohlsson, Bodil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3118184/
https://www.ncbi.nlm.nih.gov/pubmed/21609500
http://dx.doi.org/10.1186/1472-6874-11-17
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author Gustafsson, Rita J
Ahrné, Siv
Jeppsson, Bengt
Benoni, Cecilia
Olsson, Crister
Stjernquist, Martin
Ohlsson, Bodil
author_facet Gustafsson, Rita J
Ahrné, Siv
Jeppsson, Bengt
Benoni, Cecilia
Olsson, Crister
Stjernquist, Martin
Ohlsson, Bodil
author_sort Gustafsson, Rita J
collection PubMed
description BACKGROUND: Lactobacillus species are the most often found inhabitants of vaginal ecosystem of fertile women. In postmenopausal women with low oestrogen levels, Lactobacillus flora is diminishing or absent. However, no studies have been performed to investigate the correlation between oestrogen levels and the lactobacilli in the gut. The aim of the present study was to investigate the relation in healthy women between vaginal and rectal microbial flora as well as possible variations with hormone levels. METHODS: Vaginal and rectal smears were taken from 20 healthy fertile women, average 40 years (range 28-49 years), in two different phases of the menstrual cycle, and from 20 postmenopausal women, average 60 years (range 52-85 years). Serum sex hormone levels were analyzed. Bacteria from the smears isolated on Rogosa Agar were grouped by Randomly Amplified Polymorphic DNA and identified by multiplex PCR and partial 16S rRNA gene sequencing. RESULTS: Lactobacillus crispatus was more often found in the vaginal flora of fertile women than in that of postmenopausal (p = 0.036). Fifteen of 20 fertile women had lactobacilli in their rectal smears compared to 10 postmenopausal women (p = 0.071). There was no correlation between the number of bacteria in vagina and rectum, or between the number of bacteria and hormonal levels. Neither could any association between the presence of rectal lactobacilli and hormonal levels be found. CONCLUSION: Lactobacillus crispatus was more prevalent in the vaginal flora of fertile women, whereas the Lactobacillus flora of rectum did not correlate to the vaginal flora nor to hormonal levels.
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spelling pubmed-31181842011-06-19 The Lactobacillus flora in vagina and rectum of fertile and postmenopausal healthy Swedish women Gustafsson, Rita J Ahrné, Siv Jeppsson, Bengt Benoni, Cecilia Olsson, Crister Stjernquist, Martin Ohlsson, Bodil BMC Womens Health Research Article BACKGROUND: Lactobacillus species are the most often found inhabitants of vaginal ecosystem of fertile women. In postmenopausal women with low oestrogen levels, Lactobacillus flora is diminishing or absent. However, no studies have been performed to investigate the correlation between oestrogen levels and the lactobacilli in the gut. The aim of the present study was to investigate the relation in healthy women between vaginal and rectal microbial flora as well as possible variations with hormone levels. METHODS: Vaginal and rectal smears were taken from 20 healthy fertile women, average 40 years (range 28-49 years), in two different phases of the menstrual cycle, and from 20 postmenopausal women, average 60 years (range 52-85 years). Serum sex hormone levels were analyzed. Bacteria from the smears isolated on Rogosa Agar were grouped by Randomly Amplified Polymorphic DNA and identified by multiplex PCR and partial 16S rRNA gene sequencing. RESULTS: Lactobacillus crispatus was more often found in the vaginal flora of fertile women than in that of postmenopausal (p = 0.036). Fifteen of 20 fertile women had lactobacilli in their rectal smears compared to 10 postmenopausal women (p = 0.071). There was no correlation between the number of bacteria in vagina and rectum, or between the number of bacteria and hormonal levels. Neither could any association between the presence of rectal lactobacilli and hormonal levels be found. CONCLUSION: Lactobacillus crispatus was more prevalent in the vaginal flora of fertile women, whereas the Lactobacillus flora of rectum did not correlate to the vaginal flora nor to hormonal levels. BioMed Central 2011-05-25 /pmc/articles/PMC3118184/ /pubmed/21609500 http://dx.doi.org/10.1186/1472-6874-11-17 Text en Copyright ©2011 Gustafsson et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Gustafsson, Rita J
Ahrné, Siv
Jeppsson, Bengt
Benoni, Cecilia
Olsson, Crister
Stjernquist, Martin
Ohlsson, Bodil
The Lactobacillus flora in vagina and rectum of fertile and postmenopausal healthy Swedish women
title The Lactobacillus flora in vagina and rectum of fertile and postmenopausal healthy Swedish women
title_full The Lactobacillus flora in vagina and rectum of fertile and postmenopausal healthy Swedish women
title_fullStr The Lactobacillus flora in vagina and rectum of fertile and postmenopausal healthy Swedish women
title_full_unstemmed The Lactobacillus flora in vagina and rectum of fertile and postmenopausal healthy Swedish women
title_short The Lactobacillus flora in vagina and rectum of fertile and postmenopausal healthy Swedish women
title_sort lactobacillus flora in vagina and rectum of fertile and postmenopausal healthy swedish women
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3118184/
https://www.ncbi.nlm.nih.gov/pubmed/21609500
http://dx.doi.org/10.1186/1472-6874-11-17
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