Protein Expression of Mesenchymal Stem Cells after Transfection of pcDNA3.1(−)-hVEGF(165) by Ultrasound-Targeted Microbubble Destruction

Ultrasound-targeted microbubble destruction (UTMD) has been proposed as a new technique for organ-specific gene transfer and drug delivery. This study was performed to investigate the effect of UTMD on marrow mesenchymal stem cells (MSCs) transfected with pcDNA3.1(−)-hVEGF(165).pcDNA3.1(−)-hVEGF(165...

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Detalles Bibliográficos
Autores principales: Pu, Zhaoxia, You, Xiangdong, Xu, Qiyuan, Gao, Feng, Xie, Xiaojie, Zhang, Hong, Jian'an, Wang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3118296/
https://www.ncbi.nlm.nih.gov/pubmed/21716668
http://dx.doi.org/10.1155/2011/839653
Descripción
Sumario:Ultrasound-targeted microbubble destruction (UTMD) has been proposed as a new technique for organ-specific gene transfer and drug delivery. This study was performed to investigate the effect of UTMD on marrow mesenchymal stem cells (MSCs) transfected with pcDNA3.1(−)-hVEGF(165).pcDNA3.1(−)-hVEGF(165) were transfected into the third passage of MSCs, with or without UTMD under different ultrasound conditions. Protein expression was quantified by hVEGF(165)-ELISA kit after transfection for 24, 48, and 72 hours. UTMD-mediated transfection of MSCs yielded a significant protein expression. UTMD of mechanic index (MI) 0.6 for 90 seconds led to the highest level of protein expression.

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